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Citations for Roche :
601 - 650 of 767 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Microbiology 2022Quote: ... The solution was incubated for 20 min at 37°C and 15 μl of proteinase K (20 g L-1l; Roche) was added ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... activities were measured kinetically at 37 °C according to the International Federation of Clinical Chemistry recommendations without Pyridoxal Phosphate (Roche). Total bilirubin concentration (TBIL ...
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bioRxiv - Physiology 2023Quote: ... and then incubated for 45 min at 37°C either in Hanks’ Balanced Salt solution (HBSS) or plain DMEM media supplemented with Liberase HD (200 μg/ml, Roche) and DNase I (40 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... UV cross-linked (1200J/m2) and hybridized with a DIG-labelled (GCCTAA)4 probe at 37°C using DIG Easy Hyb (Roche) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and digested at 37°C for an hour with mechanical disruption with a stir bar and enzymatic digestion with Liberase Blendzyme III (Roche), hyaluronidase (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue was cut finely and digested with an incubation of 30 minutes at 37°C with a digestion media of RPMI media and 200 U/mL Liberase TL (Roche). Following digestion tissue was kept cold on ice ...
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bioRxiv - Cell Biology 2022Quote: ... finely minced and incubated for 75 min at 37°C in RPMI supplemented with 5% penicillin-streptomycin and 0.4 mg/ml Liberase™ TM (Roche). Digest was stopped by adding an equal volume stopping solution of RPMI supplemented with 10% FCS to the digestion mixture and keeping cells on ice ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Microbiology 2022Quote: Lungs were perfused with sterile PBS and digested at 37°C for 1 h with 625 μg/mL collagenase D (Roche) and 75 U/mL DNase I (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Immunology 2022Quote: ... frozen tissue fragments were thawed and incubated for 5 minutes at 37°C in a dissociation buffer containing RPMI-1640 supplemented with 10 U/mL DNase (Roche), 200 U/mL collagenase IV (Worthington Biochemical) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche) and 0.1 mg/mL DNAse I ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C), the lysate (IN, input) was transferred to either 50 µl of HA bead slurry (anti-HA affinity matrix, Roche) or 50 µl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Immunology 2022Quote: ... Excised tumors were cut into small pieces and digested at 37°C for 30 min with 100 μg/ml DNase I (Roche) and 200 U/ml Collagenase (Worthington) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
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bioRxiv - Genetics 2023Quote: ... for 30 min at room temperature and incubated for 2 h at room temperature or overnight at 4°C with primary antibodies against GFP (Roche), androgen receptor (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... lower and upper mouse and hamster first molars were dissected as above and treated for 15 minutes at 37°C with Dispase (Roche) 10mg/ml in Hepes/KOH 50mM ph7.7 ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were hybridized with digoxigenin (DIG)-labeled riboprobes overnight at 65°C and incubated overnight with 1/2000 alkaline phosphatase-conjugated anti-DIG Fab fragment (Roche) at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected in ice-cold PBS and then digested with 4mg/ml Collagenase Type IV/ 0.2mg/ml DNase I /10% FCS/PBS under constant shaking with 700 rpm at 37°C for 12-15 min (Collagenase Type IV, Cat#: 17104-019, Gibco; DNase I, Cat#: 10104159001, Roche). Samples were filtered using a 70µm nylon filter and then washed twice with FACS buffer (0.5% FCS/2mM EDTA in PBS ...
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bioRxiv - Immunology 2023Quote: ... were isolated from spleens of naïve WT C57BL/6 mice by cutting samples into small pieces and incubating at 37°C for 45 minutes with 1U/ml DNase I (Roche) and 10U/ml collagenase D (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized at 37°C for 2–4 hrs in digoxigenin-synthesis reaction mixture with T7 RNA polymerase (Roche). After synthesis ...
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bioRxiv - Immunology 2023Quote: The concentration of serum free testosterone was calculated via the Vermeulen equation [34] using the concentration of serum sex hormone binding globulin and albumin determined using a Cobas C system (Roche) via electrochemiluminescence (Elecsys SHBG ...
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bioRxiv - Cell Biology 2023Quote: ... livers were perfused with perfusion buffer (37°C) followed by perfusion with buffered collagenase (Liberase TM (Hoffman La-Roche, Switzerland). Parenchymal cells were removed using low-speed centrifugation at 50xg for 2 min ...
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bioRxiv - Pathology 2023Quote: ... The preparations were washed with buffer 1 and incubated for 2h at 37°C with an anti-digoxigenin antibody conjugated to alkaline phosphatase (1:200 in blocking solution; 11093274910, Roche). Next ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Immunology 2023Quote: ... the slides were exposed to a temperature of 70L°C in a pre-formulated EZ Prep solution (Roche Diagnostics, Canada), followed by a subsequent incubation at 95L°C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and the left lobes of the lungs were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were pelleted at 1,400g for 5 min at 4 °C and lysed in 5Lml LB2 (10LmM Tris-Cl pH 8.0, 200LmM NaCl, 1LmM EDTA, 0.5LmM EGTA, Roche protease inhibitors) for 10 min at room temperature with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were pelleted by centrifugation (750 x g, 2 min, 4 °C) and resuspended in 50 μl vPBS containing EDTA-free protease inhibitor (Roche). The cells were fixed by addition of 0.5 ml ice-cold fixation solution (4% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... Typhi harboring the different transcriptional reporters were grown at 37 °C in LB supplemented with glucose-6-phosphate (G6P) (Roche) (concentrations ranging from 7.6 μM to 10 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... and nuclei extracted overnight at 4 °C in 0.35M/0.6M buffer supplemented with a protease inhibitor cocktail (Roche Cat #05056489001). ChIP was performed with anti-H3K4me2/ anti-H3K9me3 antibody ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were harvested and stored at -80°C in ICD lysis buffer (300 mM NaCl, 50 mM Tris/HCl, 1 mM EDTA, 1x Protease Inhibitor (Roche), pH 7.4) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA probes were transcribed in vitro from the vector for 1.5 hours at 37°C with relevant RNA polymerase and the DIG RNA Labeling Mix (Roche #11277073910). The reaction product was verified in 0,8% agarose gel ...
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged for 5 min at 1000 rpm and stored at 4 °C until measurement for LDH activity according to the manufacturers protocol (Roche, LDH Cytotoxicity Detection Kit ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Cell Biology 2022Quote: ... The remaining tissue was digested on a shaker at 400 rpm for 40 min at 37°C in 100 μg/ml Liberase TM (Roche, 5401127001) and 100 μg/ml DNAse I (Roche ...