Labshake search
Citations for Roche :
251 - 300 of 739 citations for SARS Coronavirus Nucleoprotein NP His tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were lysed in 0.1% NP-40 in 150mM NaCl with protease inhibitors (Roche) and protein concentration determined as above for western blots ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0,1% sodium deoxycholate and 1% NP-40 with Complete EDTA-free protease inhibitors (Roche) and sonicated 10 s on ice ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1% NP-40) containing protease inhibitor cocktail (cOmplete, EDTA-free Protease Inhibitor Cocktail – Roche). Supernatants were collected after centrifugation at 12000 RPM ...
-
bioRxiv - Genetics 2023Quote: ... 0.2% Tergitol type NP-40) supplemented with complete EDTA-free protease inhibitor cocktail (Roche), 1 mM PMSF ...
-
bioRxiv - Genetics 2023Quote: ... 0.05% NP-40 and 10% glycerol) containing 1 mini-Complete Protease inhibitor tablet (Roche) dissolved in 10 ml of buffer ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5% NP-40 and 0.5% sodium deoxycholate) supplemented with a protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
-
bioRxiv - Biochemistry 2024Quote: ... Next, 50 µl of lytic buffer was added (2% NP-40, protease inhibitors (Roche), 0.2 µM LargeBiT (produced in house ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.2% NP-40) with 1x protease inhibitor cocktail and 1x phosphatase inhibitor (PhosSTOP, Roche) for 30 minutes on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% NP-40) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche; Basel, Switzerland). Lysates were incubated for 10 minutes on ice ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) NP-40) supplemented with 2x protease inhibitor cocktail (Roche Applied Science) for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... and 0.075% NP-40) supplemented with 1 Complete EDTA-free protease inhibitor cocktail (Roche), 20 mM beta-glycerophosphate ...
-
bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were transiently transfected with C5aR2-Venus and βarr2-Rluc8 constructs using XTG9 (Roche). 24 hours post transfection ...
-
bioRxiv - Molecular Biology 2020Quote: ... an anti-HA-tag antibody (#11867431001) was purchased from Roche, an anti-Flag-tag antibody (#012-22384 ...
-
bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag mAb (clone 3F10, Roche, no. 11867423001), rabbit anti-SEC61A mAb (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... Ulp1 and cleaved affinity tag were removed by NiNTA (Roche). The NiNTA flow through was adjusted to 250mM NaCl and purified on HiTRAP-SP (20mM HEPES pH 7.3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix, Roche) from the 2xStrep-tagged or 3xFLAG-tagged plasmids with oligonucleotide primers containing attB recombination sites and recombined into pDONR221 using BP clonase II (ThermoFisher ...
-
bioRxiv - Cell Biology 2019Quote: ... and 0.075 % NP-40 supplemented with protease inhibitor cocktail (Serva) and PhosSTOP phosphatase inhibitors (Roche)] ...
-
bioRxiv - Neuroscience 2019Quote: ... 1% NP-40 and 1 mM dithiothreitol supplemented with protease (Roche, Welwyn Garden City, UK) and phosphatase (ThermoFisher ...
-
bioRxiv - Developmental Biology 2019Quote: ... cells were lysed by the addition of 25µl of 10% NP-40 (Roche, Cat#11.754599001) and subsequent vortexing for 15 seconds ...
-
bioRxiv - Cell Biology 2022Quote: ... 15 mM MgCl2 and 1% NP-40) supplemented with Protease Inhibitor Cocktail (Roche, Basel, Switzerland), 500 μM PMSF (serine protease inhibitor) ...
-
bioRxiv - Cell Biology 2022Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate plus a complete protease inhibitor cocktail (Roche) on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... All Digitionin and NP-40 based buffers were supplemented with cOmplete protease inhibitor cocktail (Roche) prior to lysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... and lysed with ice-cold NP-40 buffer supplemented with protease and phosphatase inhibitors (Roche). Cell lysates were passed 20 times through a 25-gauge needle ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1% NP-40 and 0.5% sodium deoxycholate) with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and kept on ice for 30 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10% glycerol) containing 0.1% NP-40 and one EDTA-free cOmplete protease inhibitor tablet (Roche). Cells were lysed by sonication and cleared by centrifugation for 1 h at 30,000xg ...
-
bioRxiv - Developmental Biology 2020Quote: ... NP-40 1% and Na-deoxycholate 0.25 %) supplemented with protease inhibitor cocktail (Roche, Mannheim, Germany). For immunoprecipitations ...
-
bioRxiv - Immunology 2021Quote: ... All women tested negative for antibodies against the Nucleocapsid Protein (NP) antigen (Roche Diagnostics EUA) to ensure that the antibodies detected were not produced in response to past SARS-CoV-2 infection.
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed in NP-40 lysis buffer containing 1× cOmplete Protease Inhibitor Cocktail (Roche) and 1× Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... 1% NP-40) supplemented with 0.25 mg/ml lysozyme and mini protease inhibitor cocktail (Roche). Samples were left on ice for 30 min ...
-
bioRxiv - Microbiology 2023Quote: ... 1% NP-40) supplemented with 0.25 mg/ml lysozyme and mini protease inhibitor cocktail (Roche). Samples were left on ice for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and 0.075 % NP-40 supplemented with protease inhibitor cocktail (Serva) and PhosSTOP phosphatase inhibitors (Roche)) ...
-
bioRxiv - Cell Biology 2023Quote: ... 1mM MgCl2 and 1%NP-40) supplemented with a protease inhibitor cocktail (Roche, 1:10000). Cell lysates were centrifuged at 10,000xg for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1% NP-40 substitute or TX100) supplemented with protease inhibitors (Roche cOmplete Protease Inhibitor Cocktail) and transferred to a 1.5 mL tube and rotated at 4°C for 30min ...
-
bioRxiv - Genomics 2022Quote: ... and amplified using KAPA Hi-Fi Hotstart PCR kit (Roche, KK2602). After one more step of DNA cleanup ...
-
bioRxiv - Microbiology 2020Quote: ... PCR was performed using KAPA Hi-Fi HotStart ReadyMix (KAPA Biosystems) and libraries were purified with AMPure XP magnetic beads (Beckman Coulter) ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 infection was confirmed by the RT-PCR-COBAS 6800 System (Roche Molecular Systems ...
-
bioRxiv - Genomics 2022Quote: ... Beads were washed 3 times using 400µl washing buffer (300mM NaCl, 0.1% NP 40,50mM TRIS HC1 7.5 and protease inhibitor tablets [Roche]) and twice using 400µl of TBS ...
-
bioRxiv - Cell Biology 2022Quote: ... before being detached with 40 μL of ice-cold lysis buffer (10 mM Tris-HCl pH 7.5, 148.5 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1x PhosSTOP Roche, 5x cOmplete protease inhibitor cocktail Roche ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1% deoxycholic acid and 1% Nonidet P-40 [NP-40]) containing protease inhibitor cocktail (Roche Diagnostics) and placed on ice for 30 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 million flow cytometry sorted cells were lysed in RIP buffer (25 mM Tris-Cl pH 7.4, 150 mM NaCl, 2.5 mM EDTA, 0.5 mM DTT, 0.5% NP-40, RNase inhibitor, Roche cOmplete mini EDTA-free protease inhibitor ...
-
bioRxiv - Microbiology 2022Quote: ... and 1% NP-40) supplemented with cOmplete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Roche). After centrifugation at 20,000 × g for 15 min at 4 °C ...
-
bioRxiv - Genetics 2023Quote: ... 0.05% NP-40 and 10% glycerol) containing a mini EDTA-free cOmplete protease inhibitor tablet (Roche) and UV crosslinked (3 J/cm2 ...
-
bioRxiv - Neuroscience 2023Quote: ... 0.5 % sodium deoxycholate and 1 % NP-40) supplemented with cOmplete mini EDTA-free protease inhibitors (Roche). After protein quantification using bicinchoninic acid assay (BCA ...
-
bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
-
bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
-
bioRxiv - Genomics 2022Quote: ... DNA libraries were amplified with KAPA 2× Hi-Fi Hotstart Readymix (Roche) and purified with 18% Sera-Mag Magnetic Beads in polyethylene glycol.
-
bioRxiv - Microbiology 2020Quote: ... His-tagged CopS(34-151) was purified using Ni-NTA columns (Roche)(11) ...
-
bioRxiv - Biophysics 2022Quote: ... His-tagged ecMSG was loaded onto a gravity nickel affinity column (Roche) and eluted using 300 mM imidazole ...