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Citations for Roche :
451 - 500 of 1971 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed on white 384-well plates (E&K Scientific, Santa Clara, CA) using Roche Lightcycler 480 (Roche Molecular Systems, Inc.). Each reaction contained 1 µL of cDNA from the RT reaction ...
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bioRxiv - Immunology 2023Quote: The retroviral packaging cell line Platinum-E was co-transfected with pMXs-SOX2-IP or pMXs-IP using the DNA Transfection Reagent X-TremeGene 9 (ROCHE, Cat# 06365779001) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Neuroscience 2020Quote: DNA from the patient and his parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
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bioRxiv - Cell Biology 2020Quote: ... and IFNγ (1000 U/mL) (cat. 11040596001-Roche, Basilea, Switzerland) for 24 h.
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated with sheep-anti-DIG (1/1000, Roche) followed by biotinylated donkey-anti-sheep (1/500 ...
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bioRxiv - Immunology 2022Quote: ... using 1000 ng of RNA per sample (Roche, Basel, Switzerland). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-fluorescein/DIG-Alkaline phosphatase (AP) (1:1000; Roche) antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies used: rat anti-HA 1:1000 (Roche 3f10) and anti-fibrillarin (ABCAM ab4566) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were counterstained with DAPI (1:1000; Roche Applied Science) and then mounted ...
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000 protease inhibitor pill (Roche: 04-693-132-001). For the preparation 40 g of lysed cell powder was used and a cytosolic S100 fraction was prepared ...
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bioRxiv - Cell Biology 2020Quote: ... The digestion buffer contains 1000 µg/ml Liberase TL (Roche), 100 U/ml DNase1 (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Additional antibodies are rat anti-HA (Roche, #11867431001, 1:1000), mouse anti-NeuN (Chemicon #MAB377 1:2000) ...
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bioRxiv - Plant Biology 2022Quote: ... an antibody conjugate anti-DIG-AP (1:1000 dilution, Roche) and NBT/BCIP substrate were used for coloring reaction ...
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bioRxiv - Plant Biology 2022Quote: ... An antibody conjugate anti-DIG-AP (Roche, 1:1000 dilution) and NBT/BCIP substrate were used to visualize hybridization results ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... or sheep anti DIG-POD (1:1000, Roche cat# 11207733910). Subsequently sections were incubated with Streptavidin-Cy2 and DAPI in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (IB: 1: 1000, cat#11814460001, Roche, Basel, Switzerland) and anti-αtubulin (IB ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells were lysed by sonication on ice in PBS pH 7.4 with protease inhibitors (cOmplete, EDTA-free, Roche Applied Science). The protein was purified over Glutathione Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: The open-reading frame of the nikABCDE gene was amplified from genomic DNA belonging to Escherichia coli BL21(DE3) using the KAPA-HiFi Master Mix kit according to the manufacturer’s instructions (Kapa Biosystems, #KK2601). Primers are found in Supplementary Table 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... the first of which assessed aerosol formation when handling potentially infectious samples using a panel of cobas e analyzers (conducted at Roche Diagnostics, Penzberg, Germany). The second sub-study assessed aerosol formation when handling potentially infectious samples using cobas e analyzers in an end-to-end laboratory workflow (conducted at Roche Diagnostics International ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Genomics 2020Quote: ... and high-density lipoprotein cholesterol [HDLC]) were determined enzymatically either with the COBAS Integra 400 Plus or Modular-E analyzers (Roche Diagnostics, Indianapolis, IN). Methods were standardized to in-house and other appropriate reference methods (e.g. ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (2 mM activated Na3VO4, 2 mM NaF and 1x PhosSTOP (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-Glutamine (R10) (Thermo 25030081) + 10 IU/mL IL-2 (Roche 11011456001) after CD3/CD28 stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20] and maintained for 2 hours in 2% blocking reagent (11096176001, Roche) in TNT ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM creatine phosphate (Roche), 10 ng/µL creatine kinase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% Blocking reagent (Roche, 11096176001), 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... using 2 % blocking reagent (Roche), followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750 ...
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bioRxiv - Microbiology 2020Quote: ... 2 U Lactate dehydrogenase (Roche) and 10 µg PGAM was pre-warmed to 30 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking reagent (Roche – 1096176001), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG-AP antibody (Roche – 11093274910 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2× Protease Inhibitor Cocktail (Roche), 2 mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... and 2% Nutridoma-CS (Roche) for 6 days39 ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM creatine phosphate (Roche), 10 ng μL−1 creatine kinase (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBSTr and incubated overnight at 4 °C MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-Fluorescein-POD Fab fragments serum (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated overnight at 4°C in 0.1M malic acid/0.1%-TritonX (MABTr)/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-AP Fab fragments serum (1:5000 ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were incubated overnight at 4 °C in blocking solution MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-POD Fab fragments serum (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... version 2 (Roche, Basel, Switzerland), with a lower limit of detection of 20 copies/ml of plasma.
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mg DNase I (Roche) and triton X-100 to 1% were added ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 2% blocking reagent (Roche, #11096176001), 10% heat-inactivated sheep serum (Equitech-Bio ...
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bioRxiv - Immunology 2023Quote: ... 2 μg/ml Histon (Roche), 1 μg/ml Sm/RNP (GenWay) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mg/mL BSA (Roche), 60 µg/mL catalase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM creatine phosphate (Roche), 10 µg/ml creatine kinase (Roche) ...