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Citations for Roche :
201 - 250 of 1812 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... qPCR was performed with specific primers (Table S2) and LightCycler 480 SYBR Green I Mastermix (Roche) using the LightCycler 480 II real-time PCR device (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... an anti-HA-tag antibody (#11867431001) was purchased from Roche, an anti-Flag-tag antibody (#012-22384 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag mAb (clone 3F10, Roche, no. 11867423001), rabbit anti-SEC61A mAb (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Clarified supernatants were rocked with Complete His-Tag resin (Roche), 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Ulp1 and cleaved affinity tag were removed by NiNTA (Roche). The NiNTA flow through was adjusted to 250mM NaCl and purified on HiTRAP-SP (20mM HEPES pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 4mL cOmplete His-Tag Purification Resin (Roche) for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using cOmplete His-Tag Purification Resin (Roche, Switzerland) and His-Bind Purification kit (Novagen ...
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bioRxiv - Cell Biology 2019Quote: ... 3) 2hr RT incubation in blocking solution (5% Sheep Serum, 1% Roche Blocking Buffer in PBST); 4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digoxigenin FISH signals were amplified using sheep anti-digoxigenin fluorescein fab fragments (1:50, Roche 11207741910) and fluorescein rabbit anti-sheep antibodies (1:100 ...
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bioRxiv - Biophysics 2022Quote: ... The AFS chamber was next functionalized with polyclonal anti-digoxigenin from sheep (Sigma, Roche cat. 11333089001) by injection of 50 μL of a 200 μg/mL anti-Dig solution (20 min incubation) ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were incubated with horseradish peroxidase (HRP) conjugated sheep anti-digoxigenin antibody (1:100, Roche) overnight at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin-labelled probes were then detected by incubating with sheep Anti-Digoxigenin-POD Fab fragments (Roche) at a 1:50 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... FITC-labelled probes were detected first by incubating with sheep Anti-Fluorescein-POD Fab fragments (Roche) at a 1:50 dilution ...
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bioRxiv - Microbiology 2022Quote: ... followed by biotin-conjugated anti-rat antibody (Roche, 1:8,000) and then with horseradish peroxidase-conjugated streptavidin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... bacterial artificial chromosome (BAC) clones were labeled with biotin- (Roche), digoxigenin- (Roche ...
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bioRxiv - Physiology 2021Quote: ... pre-hybridized and hybridized with a biotin-16-dUTP (Roche) labeled dsDNA probe including the whole 3’UTR and generated using detailed primers (Bidet et al. ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5mM biotin-16-UTP (Roche Life Science, Penzberg, Germany) as previously described (37) ...
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bioRxiv - Microbiology 2023Quote: ... and a biotin RNA labeling mix (cat. no. 11685597910, Roche). To create a 20 μl reaction volume ...
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bioRxiv - Developmental Biology 2021Quote: ... Signals of transcripts were detected using Anti-DIG-AP (alkaline phosphatase) and Fab fragments from sheep (Roche) and developed in Fast Red TR and naphthol-AS-MX-phosphate in 0.1 M Tris-HCl pH 8.2 (tablet set ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Molecular Biology 2021Quote: ... which was labelled with digoxygenin (DIG) and detected with sheep anti-DIG FITC (11207741910, Roche, RRID: AB_514498) and rabbit anti-sheep FITC antibodies (FI-6000 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... For NBT/BCIP staining sections were incubated overnight with sheep anti-DIG-AP (1:1000, Roche 11093274910). After washing staining was performed using NBT/BCIP in NTMT until satisfactory intensity ...
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bioRxiv - Microbiology 2023Quote: ... The target protein was purified using His-Tag Purification Resin (Roche) according to the manufacturer instructions.
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... in the presence of Biotin-16-UTP at 1 mM (Roche), as previously described (26) ...
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bioRxiv - Genomics 2019Quote: ... for biotin labeled probes and FITC-conjugated anti-digoxygenin (Roche, USA) for digoxigenin labeled probes ...
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bioRxiv - Genetics 2021Quote: ... Slides were hybridized with digoxigenin-(or biotin-) labeled (Roche, Basel, Switzerland) human COT-1 DNA (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... Bait DNA mixture was labeled with Biotin-16-dUTP (Roche, 11431692103) using a nick translation kit (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 35S rDNA was labeled with DIG or biotin-16-dUTP (Roche) using a Nick Translation Kit (Roche Diagnostics) ...
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bioRxiv - Genomics 2024Quote: ... using the Biotin and Dig Nick Translation Mix (Roche, Mannheim, Germany). Chromosome preparations were made from fibroblast cell culture as described previously61 ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
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bioRxiv - Physiology 2020Quote: ... The sections were incubated with sheep anti-DIG antibody (1:200, Roche Applied Science; cat. no 1333 089), biotinylated donkey anti-sheep antibody (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10,000; Roche Diagnostics, Germany). The color staining was carried out with chromogen substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate ...
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bioRxiv - Neuroscience 2021Quote: ... Fcgr1 mRNA signal was then detected using sheep anti-DIG antibody (1:500; Roche Diagnostics Corp., Indianapolis, IN) followed by the secondary antibody (Donkey anti-sheep IgG Alexa 488 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked in Blocking Buffer (5% sheep serum with 10% Roche Blocking Reagent 10x in MABT) for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000 ...
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bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...
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bioRxiv - Developmental Biology 2022Quote: ... blocked in TBTX/BSA/Sheep serum and then treated overnight with anti-DIG-AP antibody (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to cOmplete His-Tag purification resin (Roche), pre-equilibrated in lysis buffer and incubated at 4 °C for 6 h on a tube roller ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP and Myc-tag were detected using anti-GFP (Ref. 11814460001, Roche) and anti-Myc (either Ref ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered and incubated with His-tag purification resin (Roche) overnight at 4°C while mixing gently ...
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bioRxiv - Molecular Biology 2020Quote: Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg ...
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bioRxiv - Biochemistry 2022Quote: ... Coverslips were incubated with HA-tag primary antibody (1:200, Roche, 11867423001) in 0.1% BSA in PBS for 1 hour before subsequent incubation with antirat Alexa Fluor 546 secondary antibody (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified lysate was loaded onto a cOmplete His-tag purification column (Roche), immobilized proteins washed with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL cOmplete His-tag purification column (Roche). The column was then washed with ∼100 mL of lysis buffer and bound proteins were eluted in 25 mL of lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
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bioRxiv - Microbiology 2021Quote: ... then incubated with biotin-conjugated anti-rat antibody (Roche, diluted 1:8,000) in blocking buffer followed by horseradish peroxidase-conjugated streptavidin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... or with biotin-16-dUTP (Promo Kine) using Nick Translation Mix (Roche). The plasmid DNA containing EEEb2 ...