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Citations for Roche :
451 - 500 of 1812 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 2% SDS and 2 mg/ml protease inhibitor 18 (Complete, ROCHE MOLECULAR DIAGNOSTICS ...
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bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
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bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Genetics 2019Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche). Cells were subsequently incubated on ice for 30 minutes ...
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bioRxiv - Genetics 2023Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche), as described previously (Kemaladewi et al. ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM MgCl2, 2 mM DTT, 2 µM leupeptin, 2 mM PMSF, 250 mM sucrose, and 1× PhosSTOP phosphatase inhibitors [Roche]). The homogenate was centrifuged for 15 min at 10,000 × g at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein was equally loaded on SDS-PAGE for immunoblotting and the HA tag was detected using an anti-HA antibody (Roche, #11867423001 www.sigmaaldrich.com) and Femto chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
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bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP) and cOmplete protease inhibitors (Roche). Cells were lysed by sonication and cell debris pelleted at 25,000 rpm for 40 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Mg(OAc)2 supplemented with phosphatase and protease inhibitors (Roche) (adapted from (29)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% blocking reagent (Roche), 20% heat inactivated goat serum and then incubated overnight with anti-DIG antibody (Roche ...
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bioRxiv - Immunology 2021Quote: ... were thawed by washing twice in pre-warmed RPMI1640 medium supplemented with 20% FCS and 10 ng/mL DNAse I (Roche, 11284932001). Thawed CAR T cells were rested in cytokine-free complete medium (RPMI1640 medium containing 10% FCS and 1% penicillin-streptomycin ...
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bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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Proto-sex locus in large yellow croaker provides insights into early evolution of the sex chromosomebioRxiv - Evolutionary Biology 2020Quote: ... The DNA probes constructed from a BAC clone containing dmrt1 (supplementary note 11) were labeled with biotin-11-dUTP using a Nick Translation System (Roche Diagnostics, Basel, Switzerland). The probes were added to the hybridization buffer ...
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bioRxiv - Genetics 2022Quote: ... 1µg of DNA was labeled with digoxigenin (for chromosome III) or biotin (for chromosome V) by nick-translation according to manufacturer instructions (Roche, 11745816910 and 11745824910).
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bioRxiv - Cancer Biology 2019Quote: ... Immunostaining was carried out using the BenchMark XT (Ventana Medical Systems, Illkirch Graffenstaden, France) with the OmniMap kit (a “biotin-free” system using multimer technology, Roche, Boulogne Billancourt, France) and a Tris borate EDTA pH8 buffer for antigen retrieval ...
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bioRxiv - Cell Biology 2023Quote: ... probe was amplified by PCR as described previously (Cox et al., 1993) and labeled using the biotin-nick translation mix (Roche Applied Science, # 11745824910), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (2 mM activated Na3VO4, 2 mM NaF and 1x PhosSTOP (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-Glutamine (R10) (Thermo 25030081) + 10 IU/mL IL-2 (Roche 11011456001) after CD3/CD28 stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20] and maintained for 2 hours in 2% blocking reagent (11096176001, Roche) in TNT ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM creatine phosphate (Roche), 10 ng/µL creatine kinase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% Blocking reagent (Roche, 11096176001), 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... using 2 % blocking reagent (Roche), followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750 ...
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bioRxiv - Microbiology 2020Quote: ... 2 U Lactate dehydrogenase (Roche) and 10 µg PGAM was pre-warmed to 30 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking reagent (Roche – 1096176001), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG-AP antibody (Roche – 11093274910 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2× Protease Inhibitor Cocktail (Roche), 2 mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... and 2% Nutridoma-CS (Roche) for 6 days39 ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM creatine phosphate (Roche), 10 ng μL−1 creatine kinase (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBSTr and incubated overnight at 4 °C MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-Fluorescein-POD Fab fragments serum (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated overnight at 4°C in 0.1M malic acid/0.1%-TritonX (MABTr)/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-AP Fab fragments serum (1:5000 ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were incubated overnight at 4 °C in blocking solution MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-POD Fab fragments serum (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... version 2 (Roche, Basel, Switzerland), with a lower limit of detection of 20 copies/ml of plasma.
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mg DNase I (Roche) and triton X-100 to 1% were added ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 2% blocking reagent (Roche, #11096176001), 10% heat-inactivated sheep serum (Equitech-Bio ...
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bioRxiv - Immunology 2023Quote: ... 2 μg/ml Histon (Roche), 1 μg/ml Sm/RNP (GenWay) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mg/mL BSA (Roche), 60 µg/mL catalase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM creatine phosphate (Roche), 10 µg/ml creatine kinase (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM GTPgS (Roche, 10220647001) and 4 mM MgCl2 in BRB80 was incubated for 5 hours at 37°C ...