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Citations for Roche :
201 - 250 of 1689 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... a sandwich electrochemiluminescence immunoassay ECLIA on Cobas e 411 (Roche, Switzerland). The inter-assay coefficient of variation was < 5.06% using a frozen serum pool and < 2.36% using quality control samples from Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
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bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged CopS(34-151) was purified using Ni-NTA columns (Roche)(11) ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged ecMSG was loaded onto a gravity nickel affinity column (Roche) and eluted using 300 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... Hi-C libraries were prepared using KAPA LTP Library Preparation Kit (Roche) 65 with 12 amplification cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... The regions of interest (Table S1) were enriched using SeqCap HyperChoice Library probes (NimbleGen; Roche Diagnostics, Jakarta, Indonesia). Libraries were sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... coli total tRNA from strain MRE600 was obtained from Roche Pharmaceuticals.
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bioRxiv - Molecular Biology 2021Quote: ... GFP and Myc-tag were detected using anti-GFP (Ref. 11814460001, Roche) and anti-Myc (either Ref ...
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bioRxiv - Molecular Biology 2020Quote: Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg ...
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bioRxiv - Biochemistry 2022Quote: ... Coverslips were incubated with HA-tag primary antibody (1:200, Roche, 11867423001) in 0.1% BSA in PBS for 1 hour before subsequent incubation with antirat Alexa Fluor 546 secondary antibody (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Synthetic Biology 2021Quote: ... and DNA libraries were amplified with KAPA 2X Hi-Fi Hotstart Readymix (Roche).
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bioRxiv - Cancer Biology 2021Quote: ... Full length cDNA amplification with Hi-Fi Hotstart Readymix (Roche, cat. no. 07959079001) was completed with a 98°C incubation then 21 cycles of (98°C for 15 sec ...
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bioRxiv - Genomics 2021Quote: ... The Hi-C library was quantified using a KAPA library quantification kit (Roche), and further PCR amplification was performed using Phusion Hot Start II DNA polymerase (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... After gap repair with Kapa Hi-Fi HotStart Uracil+ DNA Polymerase (KAPA Biosystems) and Taq DNA Ligase (NEB ...
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bioRxiv - Genetics 2023Quote: ... or zebrafish genomic DNA using the Expand Hi-Fidelity PCR System (11732641001, Roche). Exact coordinates are listed in Supplemental Table 3 ...
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bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-HA tag (clone BMG-3F10, Roche 12013819001, dilution 1:100); mouse monoclonal anti-Kv1.2 (clone K14/16 ...
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bioRxiv - Biochemistry 2019Quote: The following antibodies were used in this study: anti-HA-tag (11867423001; Roche), anti-FlagM2-tag (F3165 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The preparation of β-glucuronidase from Escherichia coli was from Roche Diagnostic (Mannheim ...
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bioRxiv - Cell Biology 2022Quote: ... coli resuspensions were supplement with EDTA-free protease inhibitor tablets (Roche) and lysozyme prior to lysis via sonication ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs (for primers, see Supplemental Table S1) were performed with SYBR Green master mix (Ozyme) using a LightCycler 480 (Roche) and incubated for 2 min at 95°C with 35 cycles of 10 sec at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Handles were PCR-generated from the plasmid pSP73-JY0 (Fili et al., 2010) using appropriate oligonucleotides (Supplementary Table S1) and Bio-dUTP or Dig-dUTP (Roche), followed by restriction with PsiI or KpnI ...
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bioRxiv - Microbiology 2023Quote: ... Probes were obtained by PCR using the primers pGK-S55 and pGK-S53 (Table S1) and labelled with the PCR DIG Probe Synthesis Kit (Roche) according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments with adapters and tags were purified using KAPA Pure beads (Roche, Switzerland) at a 1:1 concentration.
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bioRxiv - Microbiology 2020Quote: ... coli Novablue were extracted using the High Pure RNA Isolation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... coli strains using the High Pure plasmid isolation kit (Roche, Basel, Switzerland). PCR amplification of short DNA fragments (<3000 bp in length ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli MRE600 total transfer ribonucleic acid was purchased from Roche (Switzerland). Biotin labeled single-stranded DNA oligonucleotides were obtained from B.G.I. ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG; Integrated DNA Technologies, Coralville, IA; Table S1) overnight at 42°C DIG Easy Hyb™ buffer (Roche). Blots were washed in 2X saline-sodium citrate (SSC ...
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bioRxiv - Molecular Biology 2020Quote: ... and UCRS Fwd and SF Rev respectively (Table S1) in PCR reactions with either KAPA HiFi DNA polymerase with high GC buffers (KAPA Biosystems) or Hot FIREPol Blend Master mix (Solis BioDyne) ...
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bioRxiv - Molecular Biology 2020Quote: ... A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG) using DIG Oligonucleotide Tailing Kit (Roche Diagnostics) in Hybridization Solution [0.50 M Na2HPO4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by PCR amplification with KAPA 2X Hi-Fi Hotstart Readymix (Roche, Cat. No. KR0370).
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bioRxiv - Biochemistry 2020Quote: ... Mouse monoclonal antibody raised against hemagglutinin (HA) tag (clone 12CA5, #11583816001) was obtained from Roche. Mouse monoclonal antibody raised against MYC tag (9B11 ...
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bioRxiv - Physiology 2022Quote: ... Plasma was collected and measured for total TAGs concentration using commercial enzymatic kits from Roche Diagnostics (10166588130 ...
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bioRxiv - Genomics 2021Quote: ... was used for PCR amplification of all tested genome fragments from AF associated loci (for primers used see Supplementary Table S1) using Expand High Fidelity PCR system (Roche ref. 11732650001). Primers were design using NEBuilder assembly tool to have 20-basepair (bp ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were set up in 20 µL with 300 nM of oligonucleotides (Table S1) and LightCycler® 480 SYBR Green I Master 2x (Roche, Germany) using three technical replicates per sample ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli total tRNA mixture (from strain MRE600) and phosphoenolpyruvate were purchased from Roche Applied Science ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli total tRNA mixture (from strain MRE600) and PEP was purchased from Roche Applied Science (Indianapolis ...
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bioRxiv - Cell Biology 2020Quote: ... coli liquid cultures using the High Pure plasmid isolation kit (Roche, Basel, Switzerland).
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bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Immunology 2021Quote: ... 3 × 104 HMEC-1 cells were seeded into an E-16 multi-well plate (Roche) in triplicate and incubated for 72 h ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The screening for hCas9 presence was performed using primers reported in Data S1 by PCR using KAPA HIFI Taq (Kapa Biosystems, Boston, USA) with the following program ...