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Citations for Roche :
101 - 150 of 1588 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 12 µl of complete His-Tag Purification Resin (Roche) was added and incubated for 15 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... or Complete His-Tag Purification Resin (Roche, Basel, Switzerland), that had been prewashed with respective lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... HCMV glycoprotein B gene (gB) in the DNA sample was amplified in Lightcycler 480 real time PCR platform (Roche Molecular System, Pleasanton, CA) using TaqMan probe (LightCycler TaqMan Master ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The RBD fragment was amplified via polymerase chain reaction using Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) and then Gibson assembled in frame with an N-terminal His6-SUMO tag in the same vector as above ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche). Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL ...
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bioRxiv - Microbiology 2020Quote: ... 70-80% confluent CHO-K1 cells were transfected in 35 mm dishes using X-TREMEGENE 9 Transfection Reagent (Roche, 6365787001). Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... an anti-HA-tag antibody (#11867431001) was purchased from Roche, an anti-Flag-tag antibody (#012-22384 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag mAb (clone 3F10, Roche, no. 11867423001), rabbit anti-SEC61A mAb (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Clarified supernatants were rocked with Complete His-Tag resin (Roche), 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Ulp1 and cleaved affinity tag were removed by NiNTA (Roche). The NiNTA flow through was adjusted to 250mM NaCl and purified on HiTRAP-SP (20mM HEPES pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 4mL cOmplete His-Tag Purification Resin (Roche) for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using cOmplete His-Tag Purification Resin (Roche, Switzerland) and His-Bind Purification kit (Novagen ...
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bioRxiv - Cell Biology 2019Quote: The ade6 PCR-U (Fig S1) was done with commercial Kapa-Taq (Kapa Biosystems, Wilmington, USA) and the PCR program:
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bioRxiv - Microbiology 2023Quote: ... The target protein was purified using His-Tag Purification Resin (Roche) according to the manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: ... The regions of interest (Table S1) were enriched using SeqCap HyperChoice Library probes (NimbleGen; Roche Diagnostics, Jakarta, Indonesia). Libraries were sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to cOmplete His-Tag purification resin (Roche), pre-equilibrated in lysis buffer and incubated at 4 °C for 6 h on a tube roller ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP and Myc-tag were detected using anti-GFP (Ref. 11814460001, Roche) and anti-Myc (either Ref ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered and incubated with His-tag purification resin (Roche) overnight at 4°C while mixing gently ...
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bioRxiv - Molecular Biology 2020Quote: Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg ...
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bioRxiv - Biochemistry 2022Quote: ... Coverslips were incubated with HA-tag primary antibody (1:200, Roche, 11867423001) in 0.1% BSA in PBS for 1 hour before subsequent incubation with antirat Alexa Fluor 546 secondary antibody (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL cOmplete His-tag purification column (Roche). The column was then washed with ∼100 mL of lysis buffer and bound proteins were eluted in 25 mL of lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified lysate was loaded onto a cOmplete His-tag purification column (Roche), immobilized proteins washed with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-HA tag (clone BMG-3F10, Roche 12013819001, dilution 1:100); mouse monoclonal anti-Kv1.2 (clone K14/16 ...
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bioRxiv - Biophysics 2019Quote: ... the crude extracts mixed with cOmplete His-Tag purification resin (Roche, Basel, Switzerland) were loaded onto a polyprep chromatography column (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: The following antibodies were used in this study: anti-HA-tag (11867423001; Roche), anti-FlagM2-tag (F3165 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 µL of the Ni-NTA slurry (Roche cOmplete His-Tag purification resin) was washed with 2x1 mL of wash buffer (WB ...
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bioRxiv - Cancer Biology 2023Quote: ... and the supernatant was loaded into cOmplete™ His-Tag Purification Resin (Roche) pre-equilibrated with wash buffer ...
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bioRxiv - Biophysics 2023Quote: ... the lysate was incubated for 1h with cOmplete His-Tag Purification Resin (Roche) at 4°C ...
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bioRxiv - Genomics 2024Quote: ... The cleared lysate was loaded onto a cOmplete His-tag purification column (Roche) and the His6-Sumo3-Tn5 was eluted with a running buffer containing 300 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants after high-speed centrifugation were loaded onto His-Tag Purific Resin (Roche), washed and eluted with 50 mM Tris.HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs (for primers, see Supplemental Table S1) were performed with SYBR Green master mix (Ozyme) using a LightCycler 480 (Roche) and incubated for 2 min at 95°C with 35 cycles of 10 sec at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Handles were PCR-generated from the plasmid pSP73-JY0 (Fili et al., 2010) using appropriate oligonucleotides (Supplementary Table S1) and Bio-dUTP or Dig-dUTP (Roche), followed by restriction with PsiI or KpnI ...
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bioRxiv - Microbiology 2023Quote: ... Probes were obtained by PCR using the primers pGK-S55 and pGK-S53 (Table S1) and labelled with the PCR DIG Probe Synthesis Kit (Roche) according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) and incubated for 1 h at room temperature or 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) or Ni Sepharose High Performance (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
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bioRxiv - Microbiology 2021Quote: ... Fab was purified from cell culture supernatant by cOmplete His-Tag Purification Resin (Roche).
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bioRxiv - Neuroscience 2023Quote: ... His6-GFP and His6-mCherry proteins were purified with cOmplete His tag resin (Roche) according to the manufacturers protocols as previously described9 ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments with adapters and tags were purified using KAPA Pure beads (Roche, Switzerland) at a 1:1 concentration.
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Genomics 2024Quote: ... the dialyzed sample was loaded again onto a cOmplete His-tag purification column (Roche), and the untagged Tn5 was collected in the flow-through ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG; Integrated DNA Technologies, Coralville, IA; Table S1) overnight at 42°C DIG Easy Hyb™ buffer (Roche). Blots were washed in 2X saline-sodium citrate (SSC ...
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bioRxiv - Molecular Biology 2020Quote: ... and UCRS Fwd and SF Rev respectively (Table S1) in PCR reactions with either KAPA HiFi DNA polymerase with high GC buffers (KAPA Biosystems) or Hot FIREPol Blend Master mix (Solis BioDyne) ...
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bioRxiv - Molecular Biology 2020Quote: ... A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG) using DIG Oligonucleotide Tailing Kit (Roche Diagnostics) in Hybridization Solution [0.50 M Na2HPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse monoclonal antibody raised against hemagglutinin (HA) tag (clone 12CA5, #11583816001) was obtained from Roche. Mouse monoclonal antibody raised against MYC tag (9B11 ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was affinity-purified by incubating it with cOmplete His-Tag purification resin (Roche) for 2 hours (4°C) ...