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Citations for Roche :
101 - 150 of 3461 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR analysis was conducted using anti-SARS-CoV-2 primer pairs specific to the E-gene (Corman et al. 2020) and Lightmix Modular SARS and Wuhan CoV E-gene (Roche Lifescience, Basel, Switzerland) in an ABI 7500 Fast PCR cycler (PE applied biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries were amplified with KAPA 2× Hi-Fi Hotstart Readymix (Roche) and purified with 18% Sera-Mag Magnetic Beads in polyethylene glycol.
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... His (Roche), GRASP55 (Proteintech) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of PCR gene specific primer (10X conc) (Table S1) and 10 μl of master mix (Roche 04707516001) in 20 μl reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... HA tag (Roche, #12013819001 or Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: HA tag (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... coli 23S rRNA spike-in (Roche, 10206938001) using RT-qPCR (20 picograms of E ...
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bioRxiv - Cancer Biology 2019Quote: ... HA-tag (Roche, 11867423001) IKKɛ (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... HA-tag (Roche, 11867423001), p53 (Santa Cruz sc-126) ...
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bioRxiv - Molecular Biology 2023Quote: ... HA-tag (Roche, 11867423001), p53 (Santa Cruz sc-126) ...
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bioRxiv - Biochemistry 2019Quote: ... The Bacmid DNA was purified and transfected into SF9 insect cells with Xtreme gene 9 DNA transfection reagent (Roche). The first virus generation ...
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bioRxiv - Microbiology 2021Quote: ... after lysis of the insect cell pellet by 3 cycles of freeze-thaw in the presence of Complete protease inhibitor cocktail (Roche), and removal of debris the lysate was loaded onto a 20-40% sucrose density gradient ...
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bioRxiv - Microbiology 2021Quote: ... and transfected with plasmid pIIIH5red-SARS-2-S DNA using X-tremeGENE HP DNA Transfection Reagent (Roche Diagnostics, Penzberg, Germany) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-HA Tag (Roche #11867423001) and anti-AIF (CST #5318 ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (clone 3F10, Roche), HA-tag (COVANCE MMS-101R) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Bioengineering 2019Quote: ... Primer sequences (S1 Table) were designed with the ProbeFinder software (Roche), and all reactions were run in triplicate.
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bioRxiv - Cancer Biology 2024Quote: ... DNA was digested with 100 U/μg of S1 nuclease (Roche) at 37°C for 1 hour and was processed with the ChargeSwitch PCR Clean-up Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HA tag (clone 3f10, 11867423001, Roche), GFP (PABG1 ...
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bioRxiv - Immunology 2022Quote: ... Tags were removed by Enterokinase (Roche) treatment and purified away by Ni-NTA (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Tags were removed by Enterokinase (Roche) treatment and purified away by Ni-NTA (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... the YFP tag (Roche, 1:1000) and aldolase (rabbit ...
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bioRxiv - Plant Biology 2020Quote: ... the HA tag (Roche, Mannheim, Germany) and LHCB5 (No ...
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bioRxiv - Biochemistry 2022Quote: ... HA-tag (1:5000, Roche, 11867423001). The secondary antibodies used were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-HA tag (Roche, 11867432001), rabbit anti-FAM134B (Sigma Prestige ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Plant Biology 2024Quote: ... and 1/5000 anti-His (11667475001, Roche) for GFP and His-Tag detection ...
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bioRxiv - Neuroscience 2020Quote: ... rat monoclonal anti-HA tag (Roche, 3F10); rabbit anti-Satb1(Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... or rat anti-HA-tag (Roche Diagnostics) or mouse anti-FlagM2 and rabbit anti-APP-CTF (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... HA-epitope-tag (Roche, rat, 1:100), Discs Large (DSHB ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-HA tag (Roche, RRID: AB_2314622), and rabbit anti-HA Tag (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-HA tag (Roche; RRID, AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-HA tag (Roche, RRID: AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... Rat anti-HA tag (cat #11867423001, Roche), Sheep anti-PPM1H (DA018 ...