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Citations for Roche :
1 - 50 of 4826 citations for S Adenosyl Methionine SAMe CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... with 35S-labeled methionine (Roche). In vitro translated target proteins were incubated with Flag-tagged Swi6 at 4 °C for 20 min ...
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis and PCR amplification were carried out in the same tube using the qScript One-Step SYBR Green qRT-PCR Kit (Quantabio) and a LightCycler480 II (Roche). All reactions were performed in triplicate ...
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bioRxiv - Genomics 2023Quote: High quality short-read libraries were also generated and sequenced from the same DNA samples using a Kapa HyperPrep kit (Roche) and a DNBseq G50 platform by BGI Genomics (Hong Kong ...
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bioRxiv - Plant Biology 2019Quote: ... An Illumina DNAseq library was constructed for polishing the PacBio based assembly using 1ug of DNA the same high molecular weight DNA prep with the KAPA HyperPrep Kit (Kapa Biosystems). The Illumina DNAseq library was sequenced on an Illumina HiSeq4000 under paired end mode (150 bp ...
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bioRxiv - Plant Biology 2021Quote: ... before resuspending in the same buffer supplemented with protease inhibitor (Roche). Cells were then lysed using a BioNebulizer (Glas-Col ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... followed by the same perfusion buffer containing collagenase D and B (Roche) and protease XIV (Sigma ...
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in the same buffer supplemented with a protease inhibitor cocktail (Roche). Cells were disrupted by sonication on ice (40% power ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using a One-step BrightGreen RT-qPCR Kit (Abm, G471-S) following the manufacturer’s protocol in the LightCycler 96 Real-Time PCR System (Roche). Isocitrate dehydrogenase (ICDH ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in RIPA buffer (same as above) containing Complete Protease Inhibitor (Roche). Extracts were sonicated at high amplitude with three 10-s bursts ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The antisense RNA probes corresponding to the 3’ s-end portion of glnA CDS were prepared by the DIG RNA Labeling Kit (Roche).
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with intermittent shaking (30 s shaking, 30 s off) for 15 min with 15 units of DNAse I (Roche) to remove DNA traces ...
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bioRxiv - Microbiology 2020Quote: ... followed by 45 cycles of amplification under the following conditions: 94°C for 15 s and 60°C for 60 s in a LightCycler 96 (Roche).
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bioRxiv - Microbiology 2021Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Microbiology 2023Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Biophysics 2020Quote: ... gamma-S-ATP was added to 100μM (Roche) (ATP concentration is 500μM ...
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bioRxiv - Cell Biology 2020Quote: ... Same amount of protein extract was digested with chymotrypsin (Catalog No. 11418467001, Roche, Basel, CHE) (chymotrypsin ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche). The next day the embryos were washed in TBST ...
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bioRxiv - Biophysics 2023Quote: ... Transfections were performed on the same day using FuGENE6 HD Transfection Reagent (Roche Applied Science). Channel constructs were co-transfected with pGreen-Lantern (referred to as GFP in the text ...
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bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
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bioRxiv - Microbiology 2021Quote: ... Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche, 1603558) mix ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR reactions with the same concentration of cDNA were run in duplicates using LightCycler 96 (Roche). Hprt was used as a housekeeping gene and used as endogenous control ...
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bioRxiv - Cancer Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-POD antibodies (1:500, Roche) and revealed using the kit TSA-Plus Cyanin-3 (Perkin Elmer).
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bioRxiv - Biophysics 2023Quote: ... Transfections were performed on the same day using FuGENE6 Transfection Reagent (Roche Applied Science, Madison, WI). TRP channel constructs were co-transfected with pGreen-Lantern (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Elecsys® Anti-SARS-CoV-2 S from Roche for the in vitro quantitative determination of antibodies (including IgG ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR reactions were run for 50 cycles (95 °C for 15 s and 60 °C for 45 s) on a LightCycler 480 (Roche Diagnostics, Mannheim, Germany). Each sample was examined in three technical replicates and dissociation curves were analysed to verify the specificity of each amplification reaction ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were extracted in the same buffer containing 10 µM of MG132 and anti-protease cocktail (Roche) at a concentration of 1 tablet for 10 mL ...
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bioRxiv - Cell Biology 2020Quote: ... All buffers used were prepared on the same day and supplemented with Protease Inhibitor Cocktail (Roche, 04693132001) and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Genomics 2022Quote: ... Supernatants from the same sample were pooled into a 1.5 mL tube and 1X protease inhibitor (Roche) was added to the chromatin ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Cancer Biology 2020Quote: ... cooling: 37 °C for 30 s (LightCycler® 96, Roche). For relative quantification ...
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bioRxiv - Plant Biology 2021Quote: ... 100μM GTP-□-S and Complete EDTA-Free Protease Inhibitor (Roche), centrifuged and 1 mM DTSSP (3,3’-dithiobis-(sulfosuccinimidyl propionate) ...
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bioRxiv - Physiology 2021Quote: ... and cOmplete Mini tablets (Roche Diagnostics A/S, Mannheim, Germany). Kidney protein samples were prepared for immunoblotting using Laemmli sample buffer containing 15 mg/mL DTT.
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bioRxiv - Microbiology 2023Quote: ... 1% P/S and 50 U/mL IL-2 (Roche), at 37°C and 5% CO2.
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bioRxiv - Microbiology 2020Quote: ... 42 ℃ for 5 min and 95 ℃ for 10 s followed by 40 cycles of 95℃ for 5 s and 60 ℃ for 20 s on an LightCycler® 480 Instrument (Roche Diagnostics Ltd, Rotkreuz, Switzerland). The absolute quantification of SARS-CoV-2 RNA levels was performed by comparison to a standard curve and shown as SARS-CoV-2 RNA copies per mouse ...
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bioRxiv - Synthetic Biology 2019Quote: ... rinsed three times with the same buffer and then soaked in BM chemiluminescence blotting substrate (Roche Molecular Diagnostics). After 1 min of incubation in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... hearts were enzymatically digested by perfusing with the same solution plus 100 μM Ca2+ and Liberase TM (Roche, final 0.25 collagenase Wunsch units/ml ...
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bioRxiv - Immunology 2020Quote: ... Dako Denmark A/S) and alkaline phosphatase substrate (NBT/BCIP, Roche).
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bioRxiv - Developmental Biology 2023Quote: ... 1% P/S and 1 mg ml-1 collagenase A (Roche) for 90 minutes ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: ... Tissue from Casp-8WT and Casp-8ECko pups from the same litter were pooled and digested in collagenase I/DNAse I (0.1mg/ml, Roche) at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The collected cDNA followed the same operation to complete the following qRT-PCR with SYBR Green (04913914001, Roche, Sweden) as the dye ...
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bioRxiv - Genomics 2020Quote: ... Illumina adapters and indices were added in the same one-step PCR reaction using the KAPA HiFi HotStart ReadyMix (Roche). The PCR product was purified using the QIAQuick PCR Purification Kit (Qiagen ...