Labshake search
Citations for Roche :
651 - 687 of 687 citations for Recombinant Rat Nxph1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against rat anti-HA clone 3F10 (Roche) or rabbit anti-TgSKP1 UOK75 [19]
-
bioRxiv - Biochemistry 2019Quote: ... The antibodies used for the brain-ring gland complex included a rat anti-HA high-affinity monoclonal antibody (3F10, 1:20 dilution; Roche), a guinea pig anti-Shroud antibody (67 ...
-
bioRxiv - Cell Biology 2021Quote: ... Retinas from adult Long Evans rats were dissected in DMEM/F12 media supplemented with complete protease inhibitors (Roche, Indianapolis, IN) and positioned above a disc of nitrocellulose disc oriented photoreceptor-side down ...
-
bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
-
bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
-
bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
-
bioRxiv - Microbiology 2024Quote: ... BSA in TBST (TBSTB) and probed with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche) diluted 1:1000 in TBSTB for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...
-
bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
-
bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
-
bioRxiv - Biochemistry 2020Quote: ... Blots were re-probed to detect ZMPSTE24 and Rce1 using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10000 dilution), and polyclonal rabbit anti-Rce1 [52] (1:2000 dilution) ...
-
bioRxiv - Neuroscience 2020Quote: ... Rat pups were injected with BrdU (i.p. 50 mg kg-1 day-1 BrdU in saline; Roche Diagnostics, Indianapolis, IN, USA) for 5 consecutive days ...
-
bioRxiv - Plant Biology 2021Quote: ... 150mM NaCl] supplemented with 1% Tween® 20 for 30 min before probing the membrane with either rat monoclonal anti-HA antibody (3F10, Roche) or mouse monoclonal ANTI-FLAG® antibody conjugated to HRP (M2 ...
-
bioRxiv - Plant Biology 2020Quote: ... transferred to a PVDF membrane and immunoblotted with rat monoclonal anti-HA (High Affinity, clone 3F10, Roche, www.roche.com; 1:2000 dilution) and hybridized with peroxidase conjugated goat anti-rat (Polyclonal ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by rabbit anti-Rat (AI-4001, Vector) at 1:500 for 32 min and OmniMap™ anti-Rb HRP (760-4311, Roche). Blocking was performed using casein (760-219 ...
-
bioRxiv - Neuroscience 2021Quote: ... the L4/5 DRG were rapidly harvested from the isoflurane anesthetized naïve rats and were incubated in 0.01% blendzyme 2 (Roche Diagnostics, Madison, WI) for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... falciparum ACT1 (final dilution 1:500; a kind gift from Jake Baum) or rat anti-HA (1:1000; Roche, Basel, Switzerland) for HA-tagged GAP45 and AMA1 ...
-
bioRxiv - Plant Biology 2023Quote: ... Primary and secondary antibody dilutions used for immune detection were as follows: α-HA(rat)-1:2,000 (Roche Diagnostics, Basel, Switzerland), α-GFP(mouse)-1:1500 (Roche Diagnostics ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
-
bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
-
bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for the preparation of stained gels for expansion microscopy: Rat anti-HA (3F10, 1:25, Roche 12158167001), Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500 ...
-
bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
-
bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
-
bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
-
bioRxiv - Plant Biology 2023Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA [Roche], rat, 1:3000). Excess primary antibody was then removed by washing three times in 2x TBST (150 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
-
bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were then mounted to glass slides and coverslipped with Vectashield Vibrance (Vector H-1700) Primary antibodies used for immunohistochemistry (IHC) are listed here with dilutions indicated in parentheses: rat anti-HA (Roche 11867423001, 1:500), chicken anti-GFP (Abcam ab13970 1:1500) ...
-
bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
-
bioRxiv - Bioengineering 2021Quote: ... The blood glucose levels of ZDF rats in each group were measured using a digital glucose meter and test strips (Accu-Chek® Sensor, Roche Inc., Mannheim, Germany) 2 or 3 days prior to surgery and at the study endpoint ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
-
bioRxiv - Developmental Biology 2020Quote: Ovaries and testis were fixed and stained according to standard procedures with the following primary antibodies: rat anti-HA (1:500, Roche cat# 11867423001, RRID: AB_390919), rat anti-Vasa (1:100 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...