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101 - 150 of 705 citations for Recombinant Rat Il1r1 Fc His tagged since 2019

• Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance

  Reshma Thamkachy, et al., bioRxiv - Cell Biology 2023

  Quote: The TetR-eYFP tagged proteins were transfected using the XtremeGene-9 (Roche) transfection reagent according to the manufacturer’s protocol ...
• The immunophilin protein FKBPL and its peptide derivatives are novel regulators of vascular integrity and inflammation via NF-κB signaling

  Stephanie Annett, et al., bioRxiv - Immunology 2021

  Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
• Two distinct HIRA-dependent pathways handle H3.3 de novo deposition and recycling during transcription

  Júlia Torné, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-HA epitope rat monoclonal (Roche) 1:1000 ...
• The chromosomal passenger complex establishes chromosome biorientation via two parallel localization pathways

  Theodor Marsoner, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA-clone 3F10 (Roche), mouse anti-Myc monoclonal 4A6 (EMD Millipore) ...
• Coding variants identified in diabetic patients alter PICK1 BAR domain function in insulin granule biogenesis

  Rita C. Andersen, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (Roche, clone 3F10) (200 pg/ml) ...
• An EDS1-SAG101 complex functions in TNL-mediated immunity in Solanaceae

  Johannes Gantner, et al., bioRxiv - Plant Biology 2019

  Quote: ... rat α-HA (all from Roche), and α-FLAG (Sigma) ...
• A novel RAB11-containing adaptor complex anchoring myosin-5 to secretory vesicles

  Mario Pinar, et al., bioRxiv - Cell Biology 2021

  Quote: α-HA rat mAb (1/1,000) (Roche) as primary antibody ...
• Generation of photocaged nanobodies for in vivo applications using genetic code expansion and computationally guided protein engineering

  Jack M. O’Shea, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... rat anti-HA clone 3F10 (Roche) at a dilution of 1:1000 for SGR57 ...
• Differential contribution of two organelles of endosymbiotic origin to iron-sulfur cluster synthesis and overall fitness in Toxoplasma

  Sarah Pamukcu, et al., bioRxiv - Microbiology 2021

  Quote: ... Rat monoclonal antibody (clone 3F10, Roche) was used to detect HA-tagged proteins ...
• Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit α₂δ-1 but not α₂δ-3

  James O. Meyer, Annette C. Dolphin, bioRxiv - Neuroscience 2021

  Quote: ... anti-HA Ab (rat monoclonal, Roche), anti-CaV2.2 II-III loop Ab (rabbit polyclonal ...
• Myosin 10 and a Cytoneme-Localized Ligand Complex Promote Morphogen Transport

  Eric T. Hall, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (1:250; Roche), mouse anti-CD63 (E-12 ...
• The Plasmodium falciparum CCCH zinc finger protein ZNF4 plays an important role in gametocyte exflagellation through the regulation of male gametocyte enriched transcripts

  Borja Hanhsen, et al., bioRxiv - Cell Biology 2022

  Quote: ... rat anti-HA (Roche, Basel, Switzerland), mouse anti-GFP (Roche ...
• Disrupting the plastid-hosted iron-sulfur cluster biogenesis pathway in Toxoplasma gondii has pleiotropic effects irreversibly impacting parasite viability

  Eléa A. Renaud, et al., bioRxiv - Microbiology 2022

  Quote: ... Rat monoclonal antibody (clone 3F10, Roche) was used to detect HA-tagged proteins ...
• Glia-neuron coupling via a bipartite sialylation pathway promotes neural transmission and stress tolerance in Drosophila

  Hilary Scott, et al., bioRxiv - Neuroscience 2022

  Quote: ... rat anti-HA (1:1000, Roche) The following secondary antibodies were used ...
• Transcriptional activity is shaped by the chromatin landscapes in Arabidopsis

  Bhagyshree Jamge, et al., bioRxiv - Genomics 2022

  Quote: ... Rat anti-HA antibody (Roche 3F10) was used at 1:2,000 dilution ...
• Drosulfakinin signaling modulates female sexual receptivity in Drosophila

  Tao Wang, et al., bioRxiv - Neuroscience 2021

  Quote: ... Rat anti-HA (1:100; Roche), mouse anti-GFP-20 (1:100 ...
• Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster

  M. Alessandra Vigano, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (1:200, Roche), mouse anti-Flag (1:500 ...
• Cysteine crosslinking in native membranes establishes the transmembrane architecture of Ire1

  Kristina Vaeth, et al., bioRxiv - Biochemistry 2021

  Quote: ... rat anti-HA monoclonal (3F19) (Roche), mouse anti-Dpm1 monoclonal (5C5A7 ...
• Ankyrin2 is required for neuronal morphogenesis and long-term memory and interacts genetically with HDAC4

  Silvia Schwartz, et al., bioRxiv - Genetics 2021

  Quote: ... rat anti-HA (Roche, 1:1,000) and mouse anti-α-tubulin (12G10 clone ...
• The late ISC pathway interactome reveals mitosomal-cytoplasmic crosstalk in Giardia intestinalis

  Alžběta Motyčková, et al., bioRxiv - Cell Biology 2022

  Quote: ... and rat anti-HA antibody (Roche). The release to mitosomal proteins was quantified by ImageJ (86).
• STING activation depends on ACBD3 and other phosphatidylinositol 4-phosphate-regulating proteins

  Rutger D. Luteijn, et al., bioRxiv - Immunology 2022

  Quote: ... rat-anti-HA (clone 3F10, Roche cat ...
• Nuclear architecture protein Distal antenna balances genome-binding and phase-separation properties to regulate neuroblast competence

  Gillie Benchorin, et al., bioRxiv - Developmental Biology 2022

  Quote: ... anti-HA (Rat, #11867423001 Sigma Roche), anti-Dan (Rabbit ...
• Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-calmodulin interaction by various in vitro and in vivo protein-protein interaction assays

  Kira von Bongartz, et al., bioRxiv - Plant Biology 2023

  Quote: ... rat α-HA (Hoffmann-La Roche AG ...
• Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in Drosophila

  Andrew Liu, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rat anti-HA (1:1000, Roche), rat anti-E-cadherin (1:10 ...
• Complex-specific subunits of endosomal tethering factors determine the balance between HOPS/CORVET complexes

  Ármin Sőth, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-HA (Roche, rat, 1:1000), anti-Vps18 (Abcam ...
• The Kelch13 compartment is a hub of highly divergent vesicle trafficking proteins in malaria parasites

  Sabine Schmidt, et al., bioRxiv - Cell Biology 2023

  Quote: ... rat a-HA (1:2000) (Roche), rabbit anti-aldolase (1.2000 ...
• VCF1 is an unconventional p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

  Ann Schirin Mirsanaye, et al., bioRxiv - Molecular Biology 2023

  Quote: ... HA (11867423001, Roche, rat, 1:1000); HA (sc-7392 ...
• Histone variants shape chromatin states in Arabidopsis

  Bhagyshree Jamge, et al., bioRxiv - Genomics 2023

  Quote: ... Rat anti-HA antibody (Roche 3F10) was used at 1:2,000 dilution ...
• Translation initiation factor eIF1.2 promotes Toxoplasma stage conversion by regulating levels of key differentiation factors

  Fengrong Wang, et al., bioRxiv - Microbiology 2023

  Quote: ... rat anti-HA (1:2500; Roche), mouse anti-Ty52 (1:10000 ...
• The function of ER-phagy receptors is regulated through phosphorylation-dependent ubiquitination pathways

  Rayene Berkane, et al., bioRxiv - Cell Biology 2023

  Quote: ... rat anti-HA tag (Roche, 11867432001), rabbit anti-FAM134B (Sigma Prestige ...
• A Dynamic population of prophase CENP-C is required for meiotic chromosome segregation

  Jessica E. Fellmeth, et al., bioRxiv - Genetics 2023

  Quote: ... rat anti-HA (Roche, 1:50), mouse anti-C(3)G (1:500 ...
• Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation in Drosophila oocytes

  Jay N. Joshi, et al., bioRxiv - Genetics 2024

  Quote: ... rat anti-HA (Clone 3F10, Roche) at 1:50 ...
• Mouse genomic rewriting and tailoring: synthetic Trp53 and humanized ACE2

  Weimin Zhang, et al., bioRxiv - Genomics 2022

  Quote: ... and amplified using KAPA Hi-Fi Hotstart PCR kit (Roche, KK2602). After one more step of DNA cleanup ...
• The structure and diversity of strain level variation in vaginal bacteria

  Brett A. Tortelli, Amanda L. Lewis, Justin C. Fay, bioRxiv - Microbiology 2020

  Quote: ... PCR was performed using KAPA Hi-Fi HotStart ReadyMix (KAPA Biosystems) and libraries were purified with AMPure XP magnetic beads (Beckman Coulter) ...
• The Verticillium longisporum phospholipase VlsPLA₂ is a virulence factor targets host nuclei and modulates plant immunity

  Vahideh Rafiei, et al., bioRxiv - Microbiology 2023

  Quote: ... The target protein was purified using His-Tag Purification Resin (Roche) according to the manufacturer instructions.
• The E2F4/p130 repressor complex cooperates with oncogenic ΔNp73α to promote cell survival in human papillomavirus 38 E6/E7-transformed keratinocytes and in cancer cells

  Valerio Taverniti, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Detection of tagged constructs was done using: HA-peroxidase antibody (Roche, ref: 12013819001), anti- TAP antibody (Thermofisher Scientific ...
• Identification and functional characterization of the putative members of the CTDK-1 kinase complex as regulators of growth and development in the genus Aspergillus

  Z. Agirrezabala, et al., bioRxiv - Microbiology 2023

  Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
• The ubiquitin ligase HOIL-1L regulates immune responses by interacting with linear ubiquitin chains

  Carlos Gomez-Diaz, et al., bioRxiv - Cell Biology 2021

  Quote: ... supplemented with recombinant DNASE I (1000 U) (Roche, 0453628001), cOmplete protease EDTA-free inhibitor cocktail (Roche ...
• The RNA-binding landscape of HAX1 protein indicates its involvement in ribosome biogenesis and translation

  Anna Balcerak, et al., bioRxiv - Molecular Biology 2022

  Quote: ... followed by the treatment with recombinant DNase I (Roche). 1 µg of the obtained RNA was used for cDNA synthesis using Superscript III (Invitrogen) ...
• Longitudinal characterization of phenotypic profile of T cells in chronic hepatitis B identifies immune markers predicting HBsAg loss

  Shue Xiong, et al., bioRxiv - Microbiology 2020

  Quote: ... and recombinant interleukin-2 (20U/ml; Hoffmann-La Roche). Fresh medium containing IL-2 was added twice per week ...
• PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins

  Ying Liu, et al., bioRxiv - Microbiology 2020

  Quote: ... and human recombinant IL-2 (30 U/ml, Roche) for 72 h.
• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... and 10 ng/ml recombinant human IL-2 (Roche). For activation of murine CD4+ T cells ...
• Dual-specific autophosphorylation of kinase IKK2 enables phosphorylation of substrate IκBα without requiring ATP

  Prateeka Borar, et al., bioRxiv - Biochemistry 2023

  Quote: ... The recombinant murine TNF-α used was from Roche.
• AXL limits the mobilization of cholesterol to regulate dendritic cell maturation and the immunogenic response to cancer

  Meriem Belabed, et al., bioRxiv - Immunology 2023

  Quote: ... and 50 ng/mL recombinant DNase I (Roche Diagnostics) in PBS ...
• Effect of gedunin on glioblastoma proliferation and invasiveness: In vitro and in vivo approaches

  Thadeu Estevam Moreira Maramaldo Costa, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... DNase I recombinant RNase free was purchased from Roche Life Sciences (Germany).
• The hair cycle underlies regulation of Langerhans cell renewal

  Benjamin Voisin, et al., bioRxiv - Immunology 2019

  Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
• Wheat Germ Agglutinin Conjugated Fluorescent pH Sensors for Visualizing Proton Fluxes

  Lejie Zhang, et al., bioRxiv - Biophysics 2019

  Quote: Rat ventricular myocytes were isolated from adult Sprague-Dawley rats (200 – 250 g) by enzymatic (Liberase™, Roche) digestion (Colecraft et al. ...
• “TMEM16K is an interorganelle regulator of endosomal sorting”

  Maja Petkovic, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (Roche, # 11867431001, 1/1000), mouse anti-GFP (Roche ...
• ER – lysosome contacts at a pre-axonal region regulate axonal lysosome availability

  Nazmiye Özkan, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (Roche Cat# 11867423001, RRID:AB_390918), mouse anti-Pan-Neurofascin external (clone A12/18 ...
• ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatiotemporal control of the endolysosomal pathway

  Tom Cremer, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-HA (1:200, 3F10, Roche) followed by anti-Rabbit/Mouse/Rat Alexa-dye coupled antibodies (1:400 ...