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Citations for Roche :
401 - 450 of 688 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... rat α-HA 3F10 (WB 1:1000, Roche Cat. No. 11867423001), rabbit α-H3 (WB 1:8000 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade; Roche, Mannheim, Germany) overnight at 37 °C (Sechi and Chait ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd.) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 μL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 μL nuclease free water ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 mM DTT) supplemented with 0.05 U/µl RNase-free recombinant DNase I (Roche), 0.4 U/µl RNasin (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant cloned from Flavobacterium meningosepticum and expressed in Escherichia coli) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were DNase-treated by incubating the extracts with DNase I recombinant (Roche) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Plant Biology 2023Quote: ... HA-tagged RIN4a and RIN4b expressed in soybean transgenic roots were detected using monoclonal HA-antibody (Roche Diagnostics, Germany) with or without HRP-conjugate and used in 1:2500 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... then incubated with biotin-conjugated anti-rat antibody (Roche, diluted 1:8,000) in blocking buffer followed by horseradish peroxidase-conjugated streptavidin (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Antibody concentrations used were: rat anti-HA high affinity (Roche, Basel, Switzerland) (1:1000) ...
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bioRxiv - Biochemistry 2021Quote: ... MRG-1::3XHA was detected with rat anti-HA-HRP antibody (Roche) at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used for immunoblotting were rat anti-HA (1:3000; Roche), mouse anti-α-tubulin (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used: rat anti-HA-HRP (1:1000, Cat. #3F10, Roche), mouse anti-α-Tubulin (1:5000 ...
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bioRxiv - Microbiology 2020Quote: Primary antibody dilutions were used as follows: rat α-HA antibody (Roche) 1:2,000 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were probed with anti- HA rat monoclonal antibody (3F10 clone, Roche) diluted to 0.1 mg/mL in 0.1%Triton-PBS buffer and washed four times in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used were: 1 μg/mL rat α HA (Roche 3F10), 1/2000 rabbit α GFP (Abcam ab290) ...
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bioRxiv - Biochemistry 2021Quote: ... Chromosome surface spreads were immunostained with rat –-HA at 1:200 (Roche) and rabbit –-Hop1 at 1:200 (home made)) ...
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bioRxiv - Molecular Biology 2020Quote: Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg ...
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bioRxiv - Neuroscience 2019Quote: ... The primary antibodies (rat anti-HA, 1:100, Roche life science, 11867423001) were diluted in 5% BSA + PBS-TritonX (0.3 %) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibody: rat-derived monoclonal anti-HA antibody (dilution 1:250; Roche). Secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were the OmniMap anti-rat HRP (Roche, #760-4457). For Ly6G/C (Gr1 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were stained with 1:500 rat anti-HA (Roche #11867423001), followed by 1:1000 goat anti-rat 594 (Invitrogen #A11007) ...
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bioRxiv - Molecular Biology 2023Quote: ... or monoclonal rat anti-HA antibody (Roche; Basel, CH; dilution 1:200) and polyclonal rabbit anti-Pf39 antisera (dilution 1:10,000 ...
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bioRxiv - Genomics 2022Quote: ... Paraffin-embedded rat tissues were stained with antibodies for CD3 (Roche, 2GV6), CD8 (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used were the OmniMap anti-Rat HRP (Roche, 760-4457) or OmniMap™ anti-Rb HRP (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... cells were probed with rat anti-HA (clone 3F10, Roche; 1:1,000) followed by 1:10,000 anti-rat Alexa 594 secondary ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were probed with rat anti-HA (clone 3F10, Roche; 1:1,000) followed by 1:20,000 IRDye 680RD Goat anti-Rat IgG secondary antibody (LI-COR Biosciences) ...
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bioRxiv - Physiology 2023Quote: Primary antibodies and their dilutions were: rat monoclonal against HA (Roche #11867423001), 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: The following commercially available antibodies were used: rat-anti-HA (Roche, #11867431001) and rabbit-anti-GAPDH (Cell Signaling #5174) ...
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bioRxiv - Plant Biology 2024Quote: ... After incubation with rat anti-HA:HRP (3F10 clone, Roche [dilution 1:3000]) or mouse anti-Myc:HRP antibodies (9E10 clone ...
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bioRxiv - Cell Biology 2024Quote: The primary antibodies used were rat-anti-HA 3F10 (Roche, 1:25), mouse-anti-RAP1 (2.29 ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
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bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...