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Citations for Roche :
351 - 400 of 758 citations for Recombinant Rabbit TNF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Predigested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... 80-100 μL of a mix of protein A and G-Agarose (Roche) was added to the samples ...
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bioRxiv - Neuroscience 2022Quote: ... immunoprecipitated using 50µl packed Protein G beads bound with anti-GFP antibodies (Roche), and in vitro kinase assay was performed on these samples as described above ...
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bioRxiv - Biochemistry 2023Quote: ... The protein on the membrane were probed with antibodies (either anti-HA (Roche),-FLAG (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by a tryptic digestion (Roche, enzyme to protein ratio 1:50, MSgrade) and incubation at 37°C overnight in a thermal shaker at 700 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % Brij97 (v/v) and phosphatase and proteins inhibitors (Roche, # 4906837001 and # 4693132001) for 30 min under end over end rotation at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Total protein extracts were prepared in RIPA buffer with protease inhibitor cocktail (Roche). Samples were sonicated for 3 minutes alterning 30seconds ON/ 30seconds OFF ...
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bioRxiv - Cell Biology 2022Quote: Conjugation of protein or nanobody was performed with an 8-fold (Laminin; Roche) or 4-fold (R2-myc-his ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were used for immunoblot with anti-GFP (Roche #11814460001, 1:1,000), anti-RFP (Chromotek #6g6 ...
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bioRxiv - Neuroscience 2024Quote: ... Each resulting denatured protein sample was chemically reduced with 100 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated using 100 mM iodoacetamide for 1 h ...
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bioRxiv - Immunology 2023Quote: ... After adding 50[μl of 50% of Protein-G bead (Roche Applied Science) to lysates and incubation with rotation at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes (175 mg protein) were treated with one cOmplete protease inhibitor tablet (Roche) then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-rabbit/mouse IgG secondary antibody (1:2000; Roche, Cat#12015218001, Basal, Switzerland) prior to fluorescent imaging ...
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bioRxiv - Neuroscience 2020Quote: ... lysates were incubated at 4 °C overnight with rabbit anti-GFP (1:1000, Roche) and pull down was performed with magnetic proteinA beads (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Ki-67 (rabbit monoclonal, clone name 30-9, Ventana Roche, 790-4286, 1:400), cleaved caspase 3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti- GFP (gift from Oliver Gruss) and mouse monoclonal anti-GFP (Roche), mouse monoclonal anti-β actin (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... and the androgen receptor (AR) (RTU; rabbit, #760-4605, clone SP107; Roche, Basel, Switzerland). Staining was detected by the application of 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated with secondary goat anti-rabbit antibodies (OmniMap HRP kit, Roche Ventana) followed by detection with diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2021Quote: ... primary antibodies include CD3 (clone 2GV6) rabbit monoclonal (Roche Tissue Diagnostics cat#790-4341), CD20 rabbit polyclonal (Thermo Fisher Scientific cat#RB-9013) ...
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bioRxiv - Systems Biology 2023Quote: ... rabbit anti-furin antibody (1:800, 18413-1-AP, proteintech) and DAPI (10236276001, Roche). The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... GRA16HA (and other HA-tagged proteins) was detected using rat anti-HA antibodies (Roche) while GRA24MYC was detected using rabbit anti-MYC tag antibody 9E10 (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2019Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected with a mouse anti-GFP antibody (1:5000; Roche). Immunoblot results were quantified using Image J software (v1.8.0).
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were then extracted in an IP buffer with a phosphatase inhibitor (Roche, PhosSTOP), centrifuged at 1700g for 5 min and filtered through miracloth ...
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bioRxiv - Cancer Biology 2019Quote: Protein was extracted from cultured cells in RIPA buffer containing a protease inhibitor (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: Total protein was collected in radioimmunoprecipitation buffer (RIPA) with PhosSTOP (Roche Diagnostics, Mannheim, Germany) and Complete Mini protease inhibitor cocktail tablets (EMD Chemicals Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... Following a 30 min preclear using blocked Protein A or G agarose beads (Roche), the chromatin was incubated with 5 µg antibody overnight at 4 °C (antibodies are listed in Table S6) ...
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bioRxiv - Cell Biology 2019Quote: ... Treated cells were lysed in protein lysis buffer with protease and phosphatase inhibitors (Roche). Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and chromatin quality analysed using EtBr agarose gel electrophoresis (1.3 % Biozym ME Agarose ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and analysed using ethidium bromide (EtBr ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol (TS130G) in the presence of cOmplete protein inhibitor cocktail (Roche). The resulting samples were clarified by centrifugation in an SS34 rotor (27,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of 5µl (packed volume) of protein G-coupled Agarose beads (ROCHE) and continued overnight incubation at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was detected with 1:2,000 horseradish peroxidase (HRP)-conjugated anti-HA antibody (Roche).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested by adding 1 mL of Proteinase K (20 mg/mL) (Roche) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the supernatant was cleared with 50µl of Protein agarose G (Roche cat no. 11719416001) for 3 hours at 4 °C and subsequently incubated with 50 µl of Protein agarose G with corresponding primary antibody overnight at 4°C ...