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Citations for Roche :
351 - 400 of 674 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (Roche) under denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... His6-GFP and His6-mCherry proteins were purified with cOmplete His tag resin (Roche) according to the manufacturers protocols as previously described9 ...
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bioRxiv - Immunology 2023Quote: ... Pre-digested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein was extracted using SDS lysis buffer93 with cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% (v/v) Triton X-100) containing 1X protein and phosSTOP inhibitor cocktails (Roche) for 5 min on ice and cleared by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with 20 μL of resuspended volume of Protein G beads (Roche) for 2 h at 4 °C to pull down bound proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was detected with 1:3,000 horseradish peroxidase-conjugated anti-HA (Roche, cat#12013819001) or anti-GFP (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and organoids were resuspended in RIPA protein lysis buffer containing protease inhibitor cocktail (Roche) at day 7 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation of GFP/YFP-tagged proteins was performed with anti-GFP antibodies (11814460001, Roche) using a method we previously described (Ishii and Akiyoshi ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with rat α-HA (3F10, Roche), rabbit α-Myc (Cell Signaling) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated using SDS-PAGE and detected using a monoclonal antibody against GFP (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6%–15% SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: Total proteins of cells were extracted using RIPA lysis buffer (Beyotime) with Protease Inhibitor (Roche) and Phosphatase Inhibitor (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins on the beads were digested with 0.1 μg/μl sequencing grade trypsin (Roche) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were captured with 50 μl of 50% (w/v) protein-A agarose beads (Roche) for 3 hr at 4°C on a rotary shaker ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were then subsequently digested at 37°C with Lys-C (Roche Applied Science) during 4 h then with trypsin (Sequencing Grade Modified ...
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bioRxiv - Biochemistry 2019Quote: ... then protein was eluted with 100 µM imidazole in the presence of protease inhibitors (Roche) and desalted using a PD-10 column ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 ng protein was subjected to 10% SDS gel and electrotransferred onto PVDF membrane (Roche). After blocking for 1h with 3% BSA in PBS ...
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bioRxiv - Biochemistry 2019Quote: MBP Protein preparation was performed by a crushed Complete EDTA-free protease inhibitor tablet (Roche) was added to the cell pellet before being resuspended in lysis buffer (50mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Resolved proteins were then transferred from polyacrylamide gels to 0.45 μm PVDF membranes (Immobilon, Roche) at 200 V for 1 hr ...
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bioRxiv - Immunology 2019Quote: Total protein was isolated by homogenizing mouse feces in PBS with protease inhibitor cocktail (Roche). Samples were rotated at 4°C for 4 hours and centrifuged at 16,000g for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then digested by addition of 2 μL 20 mg/mL Proteinase K (Roche) and incubation for 2 h at 55° C ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected and visualized using an HA antibody conjugated to HRP (Roche). For anti-HA immunoprecipitations ...
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bioRxiv - Cell Biology 2020Quote: ... Same amount of protein extract was digested with chymotrypsin (Catalog No. 11418467001, Roche, Basel, CHE) (chymotrypsin ...
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bioRxiv - Cell Biology 2022Quote: ... and total protein extracted in ice-cold T-PER buffer containing protease inhibitors (Roche Diagnostics). Proteins were quantified by use of the BCA protein assay (Pierce ...
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bioRxiv - Plant Biology 2021Quote: ... HA and GFP-tagged fusion proteins were detected using a Peroxidase-conjugated α-HA (Roche) or α-GFP (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were detected using an HA-HRP antibody (12-013-819; 1:3,500; Roche).
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bioRxiv - Immunology 2021Quote: ... All women tested negative for antibodies against the Nucleocapsid Protein (NP) antigen (Roche Diagnostics EUA) to ensure that the antibodies detected were not produced in response to past SARS-CoV-2 infection.
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Expressed proteins were subsequently purified using a cOmplete His-Tag Purification column (1mL column, Roche) and SEC (S200 ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged proteins were detected using mouse anti-GFP antibody (Roche 1814460, 1:1000 dilution) and anti-mouse-HRP antibody (Amersham ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted with 1× RIPA buffer containing 1× complete-mini protease inhibitor (Roche: 11836170001) and 1× phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Spheroplasts were lysed by addition of 0.5% SDS and proteins degraded using proteinase K (Roche). SDS was precipitated using 1M potassium acetate and removed by centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche) and anti-FLAG M2 (Sigma) ...
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bioRxiv - Physiology 2022Quote: Protein was extracted from frozen muscle in RIPA buffer supplemented with Complete protease inhibitor (Roche) with bead homogenization using a TissueLyser II (Qiagen ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The cell lysate was pre-cleaned with protein G agarose beads (Roche Diagnostic, Basel, Switzerland) for 1 h at 4°C ...
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bioRxiv - Bioengineering 2023Quote: MYOtissues proteins were extracted in RIPA buffer supplemented with Protease Inhibitor Cocktail EDTA-free (Roche) and Benzonase by homogenization ...
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bioRxiv - Plant Biology 2023Quote: ... proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche) and Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... Copurifying proteins were analyzed by Coomassie staining or Western blotting using antibodies against HA (Roche) or the CTD of Rpb1 (8WG16 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining lysate was rotated O/N with 25 µl of Protein-A-agarose beads (Roche) and 2 µg of mouse anti-HA antibody (Supplementary Table S1) ...
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bioRxiv - Neuroscience 2023Quote: ... total protein extracts were prepared in lysis buffer supplemented with protease and phosphatase inhibitors (Roche). Proteins were quantified by bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein extracted in ice-cold T-PER buffer containing protease inhibitors (Roche Diagnostics). Proteins were quantified by use of the BCA protein assay (Pierce ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... samples were captured with 50 µL of 50% (w/v) protein-A agarose beads (Roche), and the reaction was kept at 4°C for 3 hr on a rotary shaker ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Cell Biology 2020Quote: Protein was harvested in urea ammonium bicarbonate lysis buffer with complete EDTA free protease inhibitor (Roche) and phosphatase inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 60 µg of Protein A- or G-agarose bead slurry (Roche, Germany) for 4 hours at 4 °C ...