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Citations for Roche :
151 - 200 of 819 citations for Recombinant Mouse Ctla4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... 100 μg of total RNA was incubated with recombinant DNase I (Roche, 04716728001) for 30 min at 37°C and the reaction was stopped by incubation at 75°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... Residual genomic DNA contamination was removed by treatment with recombinant DNAse I (Roche). cDNA was generated using Superscript II reverse transcriptase and Oligo d(T ...
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bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were hybridized with a digoxigenin (DIG)-tagged antisense mRNA probes detected by an anti-DIG antibody (Roche) and developed in NBT/BCIP (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... HA-and GFP-tagged proteins were detected using horseradish peroxidase–conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade; Roche, Mannheim, Germany) overnight at 37 °C (Sechi and Chait ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd.) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 μL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 μL nuclease free water ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 mM DTT) supplemented with 0.05 U/µl RNase-free recombinant DNase I (Roche), 0.4 U/µl RNasin (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant cloned from Flavobacterium meningosepticum and expressed in Escherichia coli) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were DNase-treated by incubating the extracts with DNase I recombinant (Roche) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Plant Biology 2023Quote: ... HA-tagged RIN4a and RIN4b expressed in soybean transgenic roots were detected using monoclonal HA-antibody (Roche Diagnostics, Germany) with or without HRP-conjugate and used in 1:2500 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... NeuroMab; HCN2: mouse, 75-111, NeuroMab; HCN3: mouse, 75-175, NeuroMab; HCN4: mouse, 75-150, NeuroMab; HA: mouse, 12CA5, Roche) in 1% milk powder and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Mouse (Roche) on the LightCycler 2.0 (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...