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Citations for Roche :
351 - 400 of 1366 citations for Recombinant Human TNFRSF17 protein Fc His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Both probes were labeled by random hexamer labeling with High Prime (Roche, 11585592001) and [α-32P]-dCTP ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were digoxigenin-labeled using the DIG RNA Labeling Mix purchased from Roche.
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bioRxiv - Molecular Biology 2021Quote: Antisense RNA probes labeled with digoxigenin (DIG RNA Labeling Mix 10 × conc, Roche) and biotin (Biotin RNA Labeling Mix 10 × conc ...
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bioRxiv - Developmental Biology 2022Quote: In vitro transcription was carried out with DIG or Biotin-labeled nucleotides (Roche) and hybridized transcripts were stained using sheep anti-DIG (Roche#1333089 ...
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bioRxiv - Neuroscience 2020Quote: ... RNA labeled probes were detected by an alkaline-phosphatase coupled antibody (Roche Diagnostics), and NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Neuroscience 2020Quote: ... and probes were labeled with digoxigenin-11-d-UTP (Roche Diagnostics, Indianapolis, IN). Tissue preparation ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then sequentially labeled with Anti-HA High Affinity (1:100, Roche) and Anti-Rat ...
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bioRxiv - Cell Biology 2021Quote: ... BAC DNA was labeled with dUTP-bio (DNA-Synthesis) or dUTP-dig (Roche) by nick translation (Knoll and Lichter 2005 ...
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bioRxiv - Immunology 2020Quote: ... labeled probe was visualized using NBT/BCIP substrate (Roche Diagnostics, Indianapolis, IN, USA) resulting in a blue/purple precipitate ...
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bioRxiv - Developmental Biology 2022Quote: ... antisense digoxigenin- and fluorescein-labeled riboprobes were synthesized according to manufacturer’s instructions (Roche) by using T7/SP6/T3 RNA polymerases ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Digoxigenin (DIG)-labeled RNA probes were prepared according to the manufacturer’s instructions (Roche). Whole-mount in situ hybridization was performed as previously described45 ...
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bioRxiv - Neuroscience 2020Quote: ... Dig-labeled probes were detected with an AP-conjugated anti-Dig antibody (Roche) followed by NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Microbiology 2021Quote: ... an in vitro produced and DIG-labeled gfp RNA probe (Roche, Mannheim, Germany) was prepared for the detection of gfp transcripts.
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bioRxiv - Developmental Biology 2020Quote: ... Digoxygenin labeled probes were synthesized using a RNA Labeling kit (SP6/T7; Roche). RNA probes were generated by linearization of TOPO-TA or ZeroBlunt vectors (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled riboprobes were synthesized following manufacturer’s protocol (DIG Labeling Kit, Roche). Primers used to generate the riboprobes are listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... the labeled DNAs were detected using a CSPD-based chemiluminescence detection system (Roche) (26) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Digoxigenin-labeled probes were generated using a 10X Dig labeling mix (Roche Diagnostics) and T7 RNA polymerase (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: Antisense RNA probes labeled with digoxigenin (DIG RNA Labeling Mix 10 × conc, Roche) or biotin (Biotin RNA Labeling Mix 10 × conc ...
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bioRxiv - Cell Biology 2022Quote: ... in presence of Digoxygenin-labeled nucleotides (DIG RNA Labeling Mix, Roche, Bâle, Switzerland).
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bioRxiv - Cell Biology 2022Quote: ... which was labeled with AP-conjugated sheep anti-DIG FAB fragments (11093274910, Roche) and visualized with Liquid PermanentRed (Dako) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Block was replaced with alkaline phosphatase-labeled (AP) anti-DIG Fab fragments (Roche) in Roche blocking reagent at a concentration of 1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... DIG-labeled marker DNA was used (DNA molecular weight marker III, 11218602910, Roche). Hybridization was performed with probes specific for AAV2 rep ...
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bioRxiv - Developmental Biology 2024Quote: ... then hybridized overnight at 65 °C with digoxigenin-labeled RNA probes (Roche, 11277073910). The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Genomics 2024Quote: ... for biotin-labeled probes and rhodamine-conjugated anti-digoxigenin (Roche Diagnostics, Basel, Switzerland) for digoxigenin-labeled probes ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10% FCS (P3 and P9) and treated with 0.05 mg/ml Liberase TH (Roche) and 0.2 mg/ml DNAse (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems), according to the QuikChange site-directed mutagenesis manual ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
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bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
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bioRxiv - Immunology 2023Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: A pTargeT™ vector was labeled by nick translation with digoxigenin-11-dUTP (Roche) (Byron et al. ...
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bioRxiv - Cell Biology 2020Quote: ... biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7/T3 RNA in vitro transcription kit (Ambion ...
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bioRxiv - Cell Biology 2020Quote: ... The rDNA and telomere probes were labeled by nick translation kit (Roche, Basel, Switzerland). The chromosome 3 painting probe was labeled with ATTO-550 as previously described (Albert et al. ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Custom DIG-labeled probes were generated using PCR DIG Probe Synthesis Kit (Roche #11636090910) and blots were hybridized for 16 hours at 48°C with a final concentration of 25ng/ml probe in 10ml DIG EasyHyb buffer rolling in hybridization tubes ...