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Citations for Roche :
451 - 500 of 931 citations for Recombinant Human REG4 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Total protein were extracted from cells and tissues using protein lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Protein concentrations were assayed by the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein extraction cells were resuspended in protein extraction buffer (1xPBS, 10% glycerol, 1mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail), and lysed in a bead beater ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extract was incubated overnight with anti-HA antibodies (Roche). After over-night incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were incubated with 30 µl Protein G-Agarose (Roche) per ml lysate for 1 hour at 4 °C and washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... followed by incubation with Protein G Agarose beads (Roche, 1124323301) (15μl ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl bed volume of Protein A Agarose (Roche 11134515001) was added the following day to each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitations were performed using Protein G Agarose beads (Roche, 1124323301) or anti-FLAG (M2 ...
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bioRxiv - Genetics 2022Quote: ... 40 μl of protein G agarose beads suspension (Roche 11243233001) (settled beads volume 20 μl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by Lumi-Light Plus detection reagent (Roche).
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bioRxiv - Microbiology 2021Quote: ... 100 µl of protein G-coupled agarose bead resin (Roche) were washed (2 × CL buffer 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 200 ng trypsin (Roche, Basel, Switzerland) shaking at 600 rpm at 37°C for 17 hours ...
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bioRxiv - Cell Biology 2020Quote: GFP-fusion proteins were detected using a monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted using RIPA buffer (Thermo) and proteinase (Roche) plus phosphatase (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Resuspended protein samples were digested with endoproteinases Lys-C (Roche) and trypsin (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and pre-cleared with protein A-agarose beads (Roche, Germany). Primary antibody incubations were carried out for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected with anti-HA antibody (Roche, Cat#11867423001).
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bioRxiv - Genomics 2019Quote: The gDNA libraries were prepared using a SeqCap EZ Human Exome Library v2.0 (Roche, Basel, Switzerland). Sequencing was performed with 100-bp paired-end reads by the HiSeq2000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790-4325), human PR rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole brain lysate was precleared with Protein G agarose (Roche, 11243233001) and the supernatant was immunoprecipitated with CB1R antibody overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The lysate was pre-cleared with protein G-agarose beads (Roche) and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatants were incubated and rotated with Protein A beads (Roche) with an anti-Mcm2 antibody (Bethyl ...
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bioRxiv - Plant Biology 2020Quote: ... protein blotting was performed using antibodies against GFP (Roche, Basel, Switzerland) and HA (Roche).
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bioRxiv - Microbiology 2021Quote: ... and pre-cleared with 80 μL of Protein-A agarose (Roche) and 100 μg BSA ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were pre-cleared with protein A-agarose beads (Roche) for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore ...
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bioRxiv - Microbiology 2022Quote: ... To prevent protein degradation a protease inhibitor was added (Complete, Roche). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... urinary protein level using a P800 biochemical analyzer (Roche, Mannheim, Germany), and urinary RBC and white blood cell (WBC ...
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bioRxiv - Molecular Biology 2021Quote: ... Protease inhibitor cocktail and protein phosphatase inhibitor cocktail were from Roche. MS023 (SML1555) ...
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bioRxiv - Immunology 2022Quote: ... containing phosphoSTOP and protein inhibitors cocktail (100 mg/ml, Roche 11836153001) was added to the tube at the ratio of 1 ml buffer per 100 mg tissue ...
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bioRxiv - Plant Biology 2023Quote: ... A volume containing 50 ul of Protein A - agarose beads (Roche) was then added to the extract before overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Protein visualization was performed by chemiluminescence using LumiLight western blotting (Roche) and ImageQuant 800 (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... protein was extracted with EB2 supplemented with PhosStop (Roche, Indianapolis, IN) and were subjected to IP with 15 μl of settled a-FLAG beads (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibody-chromatin complexes were recovered using protein G-agarose beads (Roche). After washing ...
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bioRxiv - Pathology 2023Quote: ... The expressed proteins were detected by using anti-HA-HRP (Roche) at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Lumi-Light Western Blotting Substrate (Roche, Switzerland). The signal was detected using the ChemiDOC MP Imaging System (Bio-rad ...
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... freshly resected human samples were cut into small fragments and digested with 0.1 mg/ml Liberase TL (Roche) and 0.1 mg/ml DNase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... human vimentin immunohistochemical staining of the paraffin sections was performed by using the Ventana Discovery XT instrument (Roche) with Ventana DAB Map detection Kit (760-124 ...