Labshake search
Citations for Roche :
201 - 250 of 581 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... and Proteinase K (Roche; 1 h at 55 °C) and DNA recovered using PCR purification kit (Qiagen).
-
bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
-
bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
-
bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 µL nuclease free water ...
-
bioRxiv - Microbiology 2020Quote: ... and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade; Roche, Mannheim, Germany) overnight at 37 °C (Sechi and Chait ...
-
bioRxiv - Microbiology 2019Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd.) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
-
bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 μL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 μL nuclease free water ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.25 mM DTT) supplemented with 0.05 U/µl RNase-free recombinant DNase I (Roche), 0.4 U/µl RNasin (Promega ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant cloned from Flavobacterium meningosepticum and expressed in Escherichia coli) was purchased from Roche Diagnostics (Mannheim ...
-
bioRxiv - Molecular Biology 2023Quote: ... All samples were DNase-treated by incubating the extracts with DNase I recombinant (Roche) at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
-
bioRxiv - Plant Biology 2023Quote: ... HA-tagged RIN4a and RIN4b expressed in soybean transgenic roots were detected using monoclonal HA-antibody (Roche Diagnostics, Germany) with or without HRP-conjugate and used in 1:2500 dilution ...
-
bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
-
bioRxiv - Immunology 2019Quote: ... for 3 h followed by 5 mM ATP (10519987001, Roche) or 20 μM nigericin (N-7143 ...
-
bioRxiv - Microbiology 2020Quote: ... and transfected 24 h later using Xtreme-GENE 9 (Roche) according to the manufacturer’s directions ...
-
bioRxiv - Developmental Biology 2019Quote: ... Blocking was performed for 1 h in blocking solution (Roche) diluted in maleic acid buffer ...
-
bioRxiv - Immunology 2020Quote: ... for 3 h followed by 5 mM ATP (10519987001, Roche) for 45 min were used as positive controls for caspase-1 and IL-1β blots ...
-
bioRxiv - Molecular Biology 2022Quote: ... 24 h) using X-tremeGENE 9 DNA Transfection Reagent (Roche) (3:1 ratio of reagent to DNA ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
-
bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
-
Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
-
bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
-
bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
-
bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
-
bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
-
ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
-
bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
-
bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
-
bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
-
bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
-
bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
-
bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
-
bioRxiv - Immunology 2023Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
-
bioRxiv - Cancer Biology 2020Quote: ... 24 h later the cells were treated with mitomycin C (Roche) for 1 h at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... Cells were stimulated 24 h after transfection using NeoRecormon® (Roche) at 5 ng/mL in DMEM while non-stimulated cells received DMEM ...
-
bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
-
bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...