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Citations for Roche :
601 - 650 of 6811 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... qPCR was performed on a Light Cycler 480 II (Roche) using the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were analyzed on a Lightcycler 480 II Instrument (Roche). ΔΔ cycle threshold values were calculated relative to mock-infected samples and NTC samples.
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bioRxiv - Immunology 2022Quote: ... Excised skin was placed in 0.5% Dispase II solution (Roche) and placed at 4ºC overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... qPCR was performed on a LightCycler 480 Instrument II (Roche) with LightCycler 480 SYBR Green I Master Kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The qPCR was performed using LightCycler 480 Instrument II (Roche) and the relative gene expression was calculated using the 2-(ΔΔCt ...
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bioRxiv - Neuroscience 2022Quote: ... DNase I grade II from bovine pancreas (Roche, Cat#10104159001) was dissolved in 1x HBSS at 10 mg/ml (10x ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was run on a Lightcycler 480 II (Roche) in technical triplicates ...
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bioRxiv - Microbiology 2023Quote: ... QPCR was performed in a LightCycler 480 Instrument II (Roche) using Fast Plus EvaGreen qPCR master mix (Biotium) ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCRs were run on LightCycler® 480 Instrument II (Roche) using 2 X SYBR green mix (Bio-rad) ...
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bioRxiv - Molecular Biology 2023Quote: Quantitative PCR was performed on a LightCycler 480 II (Roche) and analyzed with software from the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and measured using the LightCycler®480 Instrument II (Roche). Relative expression was calculated by LinRegPCR27 and the N0 values were normalized to the geometric mean of reference genes beta-actin ...
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bioRxiv - Microbiology 2023Quote: ... QPCR was performed in a LightCycler 480 Instrument II (Roche) using Fast Plus EvaGreen qPCR master mix (Biotium) ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue was incubated overnight in 0.2% dispase II (Roche) in DMEM at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... and analysed in a Lightcycler 480 II (Roche, Basel, Schweiz).
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bioRxiv - Plant Biology 2023Quote: ... using the LightCycler 480 II real-time PCR device (Roche). Each reaction volume was 10 µL and reactions were run in quadruplicate ...
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bioRxiv - Synthetic Biology 2024Quote: ... qPCR reactions were carried out on LightCycler 480 II (Roche). Quantification of RNA expression was normalized to ACTB (unless otherwise specified ...
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bioRxiv - Immunology 2023Quote: ... 10µg/ml DNase and 1.5mg/ml Dispase II (all Roche). Single-cell suspension was filtered through a 70μm filter to remove remaining undigested tissue ...
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bioRxiv - Immunology 2023Quote: Quantitative RT-PCR was performed on LightCycler 480 II (Roche) using TaqMan gene expression assay (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained using the Red Counterstain II kit (4 min, Roche), removed from the automated system and rinsed with distilled water ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2.4 U/ml dispase II (#4942078001, Roche, Switzerland), 2 mg/ml collagenase A (#1013586001 ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from patients NL and BAR was analysed using a custom fine tiling array covering the alpha- and beta-globin gene clusters and surrounding areas was used (Roche NimbleGen, Madison, WI, USA). Array design was based on NCBI Build 36.1 (hg18 ...
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and a single-cell suspension was prepared by digesting the tissue in collagenase type A (Roche) and 25 μg/ml DNase (Sigma ...
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bioRxiv - Immunology 2023Quote: ... spleens from wild-type or Axl-/- mice were digested with 500 μg/mL Liberase (Roche Diagnostics) and 50 ng/mL recombinant DNase I (Roche Diagnostics ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were run on the LightCycler® 480 Instrument II (Roche) with the program including 5 min pre-incubation at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... on a Light Cycler® 480 Instrument II (Roche Life Sciences). Relative expression levels for the mRNAs of interest were normalized to 28S or RS9 housekeeping genes ...
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bioRxiv - Immunology 2021Quote: ... according to manufacturer’s instructions on a Lightcycler 480 Instrument-II (Roche).
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed on the LightCycler 480 II instrument (Roche). Reactions were performed in triplicate ...
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bioRxiv - Plant Biology 2020Quote: ... on the LightCycler® 480 II system (Roche Applied Science, Germany) (S2 Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a LightCycler 480 II (Roche, Mannheim, Germany). The ACT1 gene served as an endogenous reference for normalizing expression levels ...
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bioRxiv - Microbiology 2021Quote: ... All reactions were then performed using a LightCycler 4800 II (Roche) with 0.4 µM of each primer and 5 ng of template RNA (10 µl total volume) ...
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bioRxiv - Microbiology 2020Quote: ... All PCRs were run on a LightCycler 480 II (LC480II, Roche) and performed according to the manufacturer’s instructions for use ...