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Citations for Roche :
51 - 100 of 1132 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Expressed proteins were subsequently purified using a cOmplete His-Tag Purification column (1mL column, Roche) and SEC (S200 ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Cell Biology 2021Quote: ... which used either the T7 DIG or T7 Fluorescein labelling kits (Roche). Wholemount in situ hybridisation on embryos was performed as per Thisse and Thisse (2008) ...
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bioRxiv - Cancer Biology 2021Quote: ... T7 promoter sequences (Roche) were added to one side of the PCR product during this PCR amplification ...
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bioRxiv - Developmental Biology 2019Quote: ... T7 RNA polymerase (Roche) and digoxigenin (DIG)-labeled NTPs (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and T7 (Roche, #10881767001) or T3 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 RNA Polymerase (Roche), 10X Transcription Buffer (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 DNA ligase (Roche) 25ng of backbone vector ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... including T7 promoters in the primers and subsequent transcription with T7 Polymerase (Roche). Primer sequences used are found in table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... HA-and GFP-tagged proteins were detected using horseradish peroxidase–conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... prior to transfection with 2 μg pcDNA3.1 encoding N-terminally HA-tagged human 1N3R tau or 1N4R tau (14) using X-tremeGENE 9 (Roche Life Sciences), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... hybridized fluorescein tagged dUTP was detected with alkaline phosphatase tagged anti fluorescein Fab fragments (Roche), revealed with CDP-Star (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... and T7 RNA polymerase (Roche), treated with RNase-free DNase I (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... using the T7 polymerase (Roche) and purified with PCR clean-up kit ...
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bioRxiv - Plant Biology 2021Quote: ... transcribed using T7 polymerase (Roche). Hybridization was performed on cross-sections as described by Neogy et al ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase (Roche). Synthesized RNA was precipitated with 0.8M ammonium acetate in 75% ethanol or 0.1M LiCl in 75% ethanol and finally resuspended in 50% formamide-50% RNase free water.
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bioRxiv - Cell Biology 2023Quote: ... T7 RNA polymerase (Roche, #10881775001) and RNase OUT (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and T7 transcription kit (Roche). 1 × 107 HEK293T cells were suspended in 1 mL RIP buffer (25mM Tris at pH7.5 ...
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bioRxiv - Physiology 2024Quote: ... T7 RNA polymerase (Roche Diagnostics), and the following cDNA fragments ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Bioengineering 2021Quote: ... pastoris culture medium containing the recombinant protein was purified by affinity chromatography Ni-NTA agarose (Roche).
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and NexCreERT2::R26R-tdT male mice received intraperitoneal injections (i.p.) of recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche) or PL (solvent solution ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Cell Biology 2022Quote: ... and His-tag (11965085001, Roche) antibodies were used to detect the proteins.
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bioRxiv - Biochemistry 2019Quote: ... anti-His-tag (11922416001; Roche), anti-vinculin (V4505 ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Zoology 2020Quote: ... and T7 polymerase (Roche Diagnostics GmbH) for the synthesis of antisense riboprobes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and T7 RNA polymerase (Roche Diagnostics). Purple/dark blue staining was detected using alkaline phosphatase-conjugated anti-DIG antibody FAB fragments (Roche Diagnostics ...
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bioRxiv - Genetics 2019Quote: ... and the T7 RNA polymerase (Roche). Embryos collected at 48 hpf were raised in the presence of 0.2 mM 1-phenyl-2-thiourea (PTU ...
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bioRxiv - Developmental Biology 2020Quote: ... or T7 RNA polymerase (Roche, Mannheim). Embryos at one-cell stage were injected with 1 nL MO or/and mRNA ...
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bioRxiv - Neuroscience 2022Quote: ... and transcribed with T7 polymerase (Roche); for the sense riboprobe ...
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bioRxiv - Systems Biology 2019Quote: ... and T3 or T7 polymerase (Roche), for in situ probes with digoxigenin or FITC labeling mix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... and T7 RNA Polymerase (Roche, Germany). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
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bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Biochemistry 2021Quote: ... cOmplete His-Tag Purification Resin (Roche) was added to the supernatant and samples were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...