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Citations for Roche :
401 - 450 of 1377 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... DIG-labeled probes were then visualized with an FITC conjugated anti-DIG antibody (Roche) and counterstained with DAPI.
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bioRxiv - Developmental Biology 2019Quote: ... directly from genomic DNA or cloned plasmid templates to incorporate DIG-labeled dUTP (Roche) using the primers described in Extended Data Table 5 ...
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bioRxiv - Neuroscience 2020Quote: ... DIG-labeled nucleotides were detected using Anti-Digoxigenin-Fluorescein (sheep polyclonal, Roche, 1:75) incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Arc pre-mRNA probes were synthesized using the digoxigenin-labeled UTP kit (Roche Diagnostics). Slides were fixed with 4% buffered paraformaldehyde and rinsed with 0.1 M PBS ...
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bioRxiv - Cell Biology 2021Quote: We immunoprecipitated EGFP-labeled imaginal disc nuclei with mouse anti-EGFP (Roche, cat. #11814460001). For immunofluorescence ...
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bioRxiv - Plant Biology 2020Quote: ... DIG-labeled sense and antisense RNA probes were synthesized with T7 RNA polymerase (Roche). For immunological detection ...
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bioRxiv - Cell Biology 2021Quote: ... using a digoxigenin-labeled probe and an anti-dig-HRP antibody (Roche Applied Science). In two-color FISH ...
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bioRxiv - Molecular Biology 2022Quote: Probes were labeled according to the Nick Translation-based DNA Probe Labeling method (Roche). Tak-1 and Tak-2 genomic DNA was extracted by CTAB method (Murray & Thompson ...
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bioRxiv - Molecular Biology 2022Quote: ... DIG-labeled probes were prepared using the DIG Oligonucleotide tailing kit (2nd generation; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: DIG-labeled DNA probes were synthesized using PCR DIG Probe Synthesis Kit (Roche #11636090910). Primers were designed to target either external (676 bp ...
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bioRxiv - Neuroscience 2020Quote: ... and detection of digoxigenin-labeled probes were carried out according to manufacturer’s protocol (Roche), using anti-digoxigenin antibody conjugated to alkaline phosphatase for detection ...
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bioRxiv - Molecular Biology 2022Quote: ... and CAT5/COQ7 were labeled with digoxigenin using DIG Northern Starter Kit (Roche Diagnostics).
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bioRxiv - Evolutionary Biology 2022Quote: ... DIG labeled riboprobes localization was detected by immunostaining with anti-DIG-Fab-AP (ROCHE) and NBT/BCIP.
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bioRxiv - Molecular Biology 2019Quote: ... and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche; 1:100 dilution). Samples were mounted using SlowFade Diamond Antifade with DAPI mounting media (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... labeled with alkaline phosphatase–conjugated sheep anti-DIG FAB fragments (11093274910, Roche, Basel, Switzerland), before visualized with Liquid Permanent Red (DAKO ...
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... and probes labeled with digoxigenin-11-dUTP were detected using anti-digoxigenin rhodamine (Roche). The preparations were counterstained using DAPI and mounted in Vectashield ...
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bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense digoxigenin (DIG)-labeled RNA probes were synthesized using DIG RNA Labeling Mix (Roche). Human and mouse ALDH1A3 (Clone ID 6208628 ...
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bioRxiv - Genetics 2019Quote: ... The incorporated BrdU was then detected using a FITC-labeled anti-BrdU antibody (Roche). Coverslips were mounted with Prolong Gold antifade plus DAPI (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... Nylon membranes were cross-linked and used for hybridization with DIG-labeled probe (Roche). Hybridization was done overnight at 65°C using hybridization solution (FastHyb-Hybridization solution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DIG labeled probes were made using SP6 or T7 transcription kits (Roche, Mannheim, Germany) from linearized plasmid or ...
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bioRxiv - Microbiology 2020Quote: ... All strains generated were verified by Southern blot analysis using digoxygenin labeled probes (Roche). For ip insertions ...
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bioRxiv - Genomics 2021Quote: ... DIG-labeled LINE DNA probe was prepared by PCR DIG Probe synthesis kit (Roche). Hybridization and autoradiography were performed according to the DIG Application Manual (Roche).
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bioRxiv - Plant Biology 2022Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3’end labeling kit (Roche Applied Science). Ten picomoles of each probe were used for each slide ...
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bioRxiv - Cell Biology 2023Quote: ... uuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). All GFP+ Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in alkaline phosphatase-labeled anti-DIG antibody (Roche; 1:600) in TNB for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). The labeled nuclei were purified using FACS (BD Aria ...
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bioRxiv - Developmental Biology 2022Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3′ end labeling kit (Roche Applied Science). 10 picomols of each probe were used for each slide ...
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bioRxiv - Plant Biology 2023Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3′ end labeling kit (Roche Applied Science). Ten picomoles of each probe were used for each slide ...
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bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled RNA probes were synthesized using a DIG northern Starter Kit (Roche, 11277073910), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and hybridized with DIG-labeled probe using DIG Easy Hyb (Roche Diagnostics, Mannheim, Germany). Salmon sperm DNA was used in the blocking step ...
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bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled probes were detected with anti-digoxigenin coupled to fluorescein isothiocyanate (FITC; Roche). Chromosome preparations were counter-stained with 4′,6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was performed using a T7 polymerase and DIG-labeled nucleotides (Roche). RNAscope was performed on sectioned zebrafish larvae as previously described (Carroll ...
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled in vitro transcription of rln3a and sst1.1 probes (11685619910, Roche). The gsc2 ...
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bioRxiv - Genetics 2024Quote: ... The incorporated BrdU was then detected using a FITC-labeled anti-BrdU antibody (Roche). Coverslips were mounted with Prolong Gold antifade plus DAPI (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified products were subjected to second strand synthesis using 0.3 µM of second strand biotinylated primer and the KAPA Hi-Fi hot start ready mix (KK2601, Roche). The second strand reaction was carried out at 95 °C for 3 minutes ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the capture Hi-C libraries was tested by Bioanalyzer and KAPA qPCR (Kapa Biosystems). Capture Hi-C libraries were analysed by 75 bp paired-end Next Generation Sequencing on an Illumina NextSeq500 (My-La ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Molecular Biology 2023Quote: ... then subject to second-strand synthesis using 1.5 μl biotinylated oligo (10 μM) and 25 μl KAPA Hi-Fi hot start ready mix 2x (KK2601, Roche). Second strand reactions were incubated at 95°C for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 400 μl sonication solution and 3.8 μg/μl of proteinase K (proK, Recombinant PCR Grade [Roche]) for fraction B2 ...
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bioRxiv - Microbiology 2022Quote: DNase treatment was performed according to the manufacturer’s protocols (RNase-free DNase I recombinant, Roche, US) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... all RNA samples used in this study were treated with recombinant RNase-free DNase I (Roche) (10 U of DNase I per 3 mg of total RNA ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...