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Citations for Roche :
451 - 500 of 1350 citations for Recombinant Human CD19 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... for synthesis of DIG-labeled probes or Fluorescein RNA Labeling Mix (Roche; Cat. No. 1685619) for synthesis of fluorescein-labeled probes ...
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bioRxiv - Neuroscience 2021Quote: ... and premixed RNA labeling nucleotide mixes containing fluorescein-labeled UTP (Roche Diagnostics, Indianapolis, IN, USA). Riboprobes were purified using G-50 spin columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with labeled probe and 250 µg/mL salmon sperm DNA (Roche) at 65°C overnight in Perfecthyb Plus Hybridization Buffer (Millipore) ...
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bioRxiv - Developmental Biology 2019Quote: DIG-labeled antisense riboprobes for the below genes were generated using DIG Labeling Kit (Roche). In situ hybridization was performed as described (Swift et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Labeled antisense RNA probes were transcribed from linearized DNA using digoxigenin-ll-UTP (Roche, USA) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2019Quote: ... Labeled antisense RNA probes were transcribed from linearized DNA using digoxigenin-11-UTP (Roche, USA), or labeled with DNP (Mirus ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescein labeled probes were detected using an alkaline-phosphatase conjugated anti-fluorescein Fab2 antibody (Roche), followed by BCIP/NBT (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... except Dig-labeled probes were detected with HRP-conjugated α-Dig (Roche, 11207733910, 1:500) in conjunction with the TSA system (Molecular Probes) ...
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bioRxiv - Neuroscience 2019Quote: ... Fluorescein-labeled probes were detected using peroxidase-conjugated anti-fluorescein antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using Cy3-tyramide conjugate ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then probed with alkaline phosphatase labeled anti-DIG antibody (Roche, Catalog# 11093274910) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then fixed again and hybridized with digoxigenin (DIG)-labeled probes (Roche, Basel, Switzerland) at 72°C overnight in hybridization solution (50% formamide ...
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bioRxiv - Neuroscience 2020Quote: In situ hybridization was performed using digoxigenin-labeled RNA probes (DIG RNA labeling kit, Roche) and alkaline phosphatase revelation (NBT/BCIP kit #SK-5400 ...
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bioRxiv - Genetics 2021Quote: ... DIG-labeled in-situ probes were synthesized using DIG RNA Labeling kits (Roche, Cat# 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
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bioRxiv - Zoology 2020Quote: ... Each gel also contained 32P-end-labeled size ladders (Molecular Weigh Marker XV, by Roche Diagnostics and 1Kb DNA ladder by New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin-labeled sense and antisense RNA probes were synthesized following manufacturer’s instructions (Roche Applied Science). Mature green fruits (21 DAA ...
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bioRxiv - Genetics 2020Quote: ... Digoxigenin-labeled probes were generated from linear plasmid DNA using DIG RNA Labeling Mix (Roche) and hybridized probes were detected with anti-digoxigenin antibody (Anti-Digoxigenin-AP ...
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bioRxiv - Microbiology 2021Quote: ... cells were labeled beforehand for 30 minutes with an anti HA antibody (3F10, Roche 11867423001) diluted at 1:200 in PBA ...
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bioRxiv - Developmental Biology 2022Quote: ... digoxigenin-labeled probes were recognized using an anti-DIG antibody coupled to peroxidase (11207733910, Roche), and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-Myc (WB, 1:2000; 9E10, Cat. #11667149001, Roche; labeled as IR for 293FT cells). Generation and validation of rabbit polyclonal antibodies against GFP was described previously(65 ...
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bioRxiv - Neuroscience 2023Quote: ... Digoxigenin (DIG)-labeled cRNA probes (riboprobes) were prepared using the DIG RNA Labeling Mix (Roche). Twenty μm coronal cryostat sections of mouse brains frozen in OCT were hybridized to DIG-labeled cRNA probes at 56°C for 13–16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Labeled exosomes were diluted with 3.5 ml PBS containing 1x Complete protease inhibitor cocktail (Roche) and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024 ...
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bioRxiv - Developmental Biology 2023Quote: ... The krang digoxigenin-labeled probe was synthesized in vitro using DIG RNA labeling kit (Roche) with T3 (sense ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...