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Citations for Roche :
301 - 350 of 1690 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The pellets were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were blocked in 5% non-fat milk or 5% Casein (Roche Diagnostics) in Tris-buffered saline with 0.1% Tween (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... were determined using the Rapid amplification of cDNA-5’ends (5’RACE) kit (Roche). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of nuclear extract of KDM3A and KDM3B endogenously tagged ESCs (prepared as above) was incubated with 10 µg of HA antibody (Roche, clone 12ca5, catalog#11583816001) for 4 hours rotating at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’/3’RACE Kit (Roche) was used to amplify the RNA obtained and the final DNA was sequenced to obtain the +1 nucleotide of str ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% blocking reagent (Roche, Germany)] containing 2.5 μg/mlCy-3-labelled telomere-specific (CCCTAA ...
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bioRxiv - Neuroscience 2020Quote: ... including 5% WesternBlocking Solution (Roche) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL SYBR green (Roche), 1 μL 10 mM of both forward and reverse primer (see Table S3 for primer sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL SYBR green (Roche), 1 μL 10 mM of both forward and reverse primer and 15 ng gDNA was mixed in a 10 μL reaction volume and loaded in a white qPCR plate with optical caps (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... midazolam (5 mg/kg, Roche) and medetomidine (0.5 mg/kg ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µg DNase I (Roche) and 1x complete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM DTT (Roche, 10708984001), 0.1 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM DTT (Roche, 10708984001), 0.1 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mM Tris (Roche, 107089176009), pH 7.4) ...
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bioRxiv - Immunology 2024Quote: ... laminin (5 µg/ml, Roche), bovine collagen I (30µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μg glycogen (Roche #10901393001) was added per sample ...
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bioRxiv - Biochemistry 2020Quote: ... and the presence of enzymes were confirmed by immunoblot using the anti-His6 antibody conjugated to peroxidase (BMG–His-1 monoclonal antibody) (Roche, Mannheim, Germany).
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.01 mg/mL of human insulin (Roche, Indianapolis, IN, USA), 10 mM HEPES (Corning ...
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bioRxiv - Cell Biology 2019Quote: ... 500mM NaCl, 1mM TCEP-HCl (ThermoScientific, 20491) pH 7.4 which was supplemented with recombinant DNase I (1000U) (Roche,04536282001), cOmplete protease EDTA-free inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol) supplemented with 5% v/v beta mercaptoethanol and 1x protease inhibitor cocktail (Roche) was added to each sample followed by centrifugation at 18,000×g at 4 °C for 10 min ...
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bioRxiv - Systems Biology 2019Quote: ... 25ml of pre-warmed to 37°C EGTA buffer followed by 25ml of pre-warmed to 37°C EBS buffer with 2.3U of Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) were cannulated into the vena cava ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Microbiology 2021Quote: ... A second DNase I treatment to remove all residual genomic DNA was performed using 30 U of recombinant DNase I (Roche) and 10 U of Protector RNase inhibitor (Roche ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... MGEs pooled from a single litter were incubated with 200 µL papain solution supplemented with 10 U/mL recombinant DNase I (Roche) for 10 minutes at 37 °C on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... An equal volume of lysis buffer and 110 U/ml of DNase (DNase I recombinant, RNase-free solution, Cat no. 4716728001, Roche) was added to the ground cell lysate followed by incubation for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Positive controls were generated by incubating some sections from control 3 dpf animals with recombinant DNAse I (400 U/ml; Roche) for 20 minutes at room temperature before the incubation in the reaction mix ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μg of trypsin (Roche, Switzerland) was added and the mixture was further incubated at 37°C overnight ...
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bioRxiv - Immunology 2019Quote: ... and 5 tablets PhosSTOP inhibitor (Roche) per 50 mL buffer ...
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bioRxiv - Neuroscience 2019Quote: ... dispase (5 U ml−1; Roche), and deoxyribonuclease II (50 mg ml−1 ...
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bioRxiv - Neuroscience 2019Quote: ... 5 mg/kg midazolam (Dormicum, Roche) and 0.05 mg/kg fentanyl (Fentanyl ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 5 μl proteinase K (Roche) in 20 mg/mL stock was added and incubation was continued at 55°C for 1 hr ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 μg/ml Laminin (Roche) pre-coated #1.5 Φ12 mm round cover glass (Fisher Scientific ...