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Citations for Roche :
201 - 250 of 750 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The open-reading frame of the nikABCDE gene was amplified from genomic DNA belonging to Escherichia coli BL21(DE3) using the KAPA-HiFi Master Mix kit according to the manufacturer’s instructions (Kapa Biosystems, #KK2601). Primers are found in Supplementary Table 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... the first of which assessed aerosol formation when handling potentially infectious samples using a panel of cobas e analyzers (conducted at Roche Diagnostics, Penzberg, Germany). The second sub-study assessed aerosol formation when handling potentially infectious samples using cobas e analyzers in an end-to-end laboratory workflow (conducted at Roche Diagnostics International ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Genomics 2020Quote: ... and high-density lipoprotein cholesterol [HDLC]) were determined enzymatically either with the COBAS Integra 400 Plus or Modular-E analyzers (Roche Diagnostics, Indianapolis, IN). Methods were standardized to in-house and other appropriate reference methods (e.g. ...
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bioRxiv - Microbiology 2020Quote: ... DNA was first eluted from the column in 100 µL DNA elution buffer and subsequently the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and finally RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Genomics 2022Quote: ... The labeled DNA fragment was ethanol-precipitated with sonicated salmon sperm DNA (Cat# D7656, Merk) and Escherichia coli tRNA (Cat# 10109541001, Roche Applied Science), and mouse Cot-1 DNA (Cat# 18440016 ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Developmental Biology 2021Quote: Digoxigenin-labelled sense and antisense RNA probes were prepared by in vitro transcriptions using recombinant plasmids of target genes made as mentioned above (Roche Life Science) and used for in situ hybridization ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were permeabilized by 0.3% triton in PBS and positive control slices were incubated with Dnase I recombinant (Roche, Basel, Switzerland, #4536282001) (1,000 units) ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A Agarose (Roche) were washed and equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membranes (Roche) in tris-glycine buffer using semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A beads (Roche) was added to lysates and incubated with rotation for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were prepared by mechanical lysis using glass beads in presence of protein inhibitors (Complete EDTA-free, Roche) and 2x phosphatase inhibitors PhosStop (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Cancer Biology 2021Quote: ... 25 μL of Protein G agarose beads (Roche) were placed into each IP sample and incubated o/n at 4 °C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). Protein samples were analysed by Western blot with a mouse anti-GFP HRP-conjugated antibody.
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After centrifuge ...
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of half a volume of SDS loading dye ...
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bioRxiv - Cell Biology 2021Quote: ... Protein bands were detected using Lumi-Light (Roche) chemiluminescence kit as per the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... 1x protein inhibitor cocktail (cOmplete, Roche; added fresh), 300μg/ml lysozyme ...
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bioRxiv - Plant Biology 2021Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of SDS loading buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... GUN1 protein was expressed in RTS ProteoMaster (Roche). Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... protein was degraded by Proteinase K (3115879001, Roche), and a phenol/chloroform/isoamyl alcohol DNA extraction ...
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bioRxiv - Cell Biology 2019Quote: ... A Protein A agarose bead slurry (Sigma-Roche) (60ul ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-G agarose beads were manufactured by Roche and purchased from Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... 750 μL of Protein G Agarose beads (Roche) were used to capture 400 μL of SUMO-2/3 antibody (8A2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2022Quote: ... Pre-equilibrated Protein G conjugated agarose beads (Roche) were added for 1 hour. ...
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bioRxiv - Biochemistry 2022Quote: ... and T4 Gene 32 Protein (Gp32; Roche 10972983001) were obtained from commercial ven-dors ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk or 5% bovine serum albumin for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ul of Protein A-agarose beads (Roche) was then added to the extract before overnight incubation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: To 2 mL of each urine sample adjusted to pH 7 by addition of 1.5 mL of phosphate buffer, 50 μL of internal standard solution (methyltestosterone, 200 ng/mL) and 50 μL of β-glucuronidase from Escherichia coli (Roche Diagnostics, Mannheim, Germany) were added ...