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Citations for Roche :
201 - 250 of 772 citations for Recombinant Bovine IL3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... After the membrane was blocked with 5% BSA (Bovine Serum Albumin Fraction V, Roche Diagnostics, Germany) in TBST buffer (1X Tris-Buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... resuspendend in 10 ml of PBS buffer with 5 μg/ml DNaseI (from bovine pancreas, Roche) and 10 μg/ml lysostaphin and incubated for 20 min at 37°C to allow cell lysis ...
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bioRxiv - Microbiology 2023Quote: ... 240 μL of 80 mg mL−1 DNase I from bovine pancreas (Roche Diagnostics, Mannheim, Germany) was added along with MgCl2 to a final concentration of 30 mM ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Developmental Biology 2021Quote: Digoxigenin-labelled sense and antisense RNA probes were prepared by in vitro transcriptions using recombinant plasmids of target genes made as mentioned above (Roche Life Science) and used for in situ hybridization ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were permeabilized by 0.3% triton in PBS and positive control slices were incubated with Dnase I recombinant (Roche, Basel, Switzerland, #4536282001) (1,000 units) ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A Agarose (Roche) were washed and equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membranes (Roche) in tris-glycine buffer using semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A beads (Roche) was added to lysates and incubated with rotation for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were prepared by mechanical lysis using glass beads in presence of protein inhibitors (Complete EDTA-free, Roche) and 2x phosphatase inhibitors PhosStop (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Cell Biology 2021Quote: ... Fatty acid-free bovine serum albumin (BSA) and Complete Protease Inhibitor cocktail tablets were procured from Roche Diagnostics (Laval ...
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bioRxiv - Cancer Biology 2023Quote: ... + 4% fetal bovine serum (FBS, Hyclone) and 0.01 mg/mL insulin-transferrin-sodium-selenite solution (ITSS; Roche) as previously described 28,29 ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Cancer Biology 2021Quote: ... 25 μL of Protein G agarose beads (Roche) were placed into each IP sample and incubated o/n at 4 °C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). Protein samples were analysed by Western blot with a mouse anti-GFP HRP-conjugated antibody.
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After centrifuge ...
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bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of half a volume of SDS loading dye ...
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bioRxiv - Cell Biology 2021Quote: ... Protein bands were detected using Lumi-Light (Roche) chemiluminescence kit as per the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... 1x protein inhibitor cocktail (cOmplete, Roche; added fresh), 300μg/ml lysozyme ...
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bioRxiv - Plant Biology 2021Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of SDS loading buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... GUN1 protein was expressed in RTS ProteoMaster (Roche). Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... protein was degraded by Proteinase K (3115879001, Roche), and a phenol/chloroform/isoamyl alcohol DNA extraction ...
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bioRxiv - Cell Biology 2019Quote: ... A Protein A agarose bead slurry (Sigma-Roche) (60ul ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-G agarose beads were manufactured by Roche and purchased from Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... 750 μL of Protein G Agarose beads (Roche) were used to capture 400 μL of SUMO-2/3 antibody (8A2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2022Quote: ... Pre-equilibrated Protein G conjugated agarose beads (Roche) were added for 1 hour. ...
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bioRxiv - Biochemistry 2022Quote: ... and T4 Gene 32 Protein (Gp32; Roche 10972983001) were obtained from commercial ven-dors ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk or 5% bovine serum albumin for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ul of Protein A-agarose beads (Roche) was then added to the extract before overnight incubation ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 μL of 20 μg/μL (1 μg BSA/reaction) of nonacetylated bovine serum albumin (BSA, 10711454001, Roche), 1 μL SuperScript™ III RT/Platinum Taq Mix and 1.85 μL of primer/probe mix (2019-nCoV CDC EUA Kit ...
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bioRxiv - Genetics 2020Quote: ... with fetal bovine serum (FBS, 10% v/v, Biowest) and Penicillin/Streptomycin (100 U/mL, Roche Diagnostics GmbH). Cells were cultured on 10 cm sterile plates in 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 80 μL DNase I (5 mg/ml; DNase I – grade II from bovine pancreas from Roche (Basel, Switzerland)) ...
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bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...