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Citations for Roche :
251 - 300 of 920 citations for Recombinant Bovine FCGRT & B2M Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... 2% fatty acid-free bovine serum albumin (BSA) fraction V (Roche), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... After blocking for 1 hour in 3% Bovine Serum Albumin (Roche) in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mg/ml RNAse-free bovine serum albumin (BSA, Roche Diagnostics) and 10% dextran sulfate (MP Biomedicals) ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added 1 uL RNase (from bovine pancreas, DNase free, Roche) and 1 uL RNase H (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and then blocked with 1% bovine serum albumin (BSA; Roche #3853143) in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues were blocked with 10% bovine serum albumin (BSA) (Roche) for 1 h at room temperature ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: ... prior to transfection with 2 μg pcDNA3.1 encoding N-terminally HA-tagged human 1N3R tau or 1N4R tau (14) using X-tremeGENE 9 (Roche Life Sciences), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the capture Hi-C libraries was tested by Bioanalyzer and KAPA qPCR (Kapa Biosystems). Capture Hi-C libraries were analysed by 75 bp paired-end Next Generation Sequencing on an Illumina NextSeq500 (My-La ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 400 μl sonication solution and 3.8 μg/μl of proteinase K (proK, Recombinant PCR Grade [Roche]) for fraction B2 ...
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bioRxiv - Microbiology 2022Quote: DNase treatment was performed according to the manufacturer’s protocols (RNase-free DNase I recombinant, Roche, US) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... all RNA samples used in this study were treated with recombinant RNase-free DNase I (Roche) (10 U of DNase I per 3 mg of total RNA ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
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bioRxiv - Cell Biology 2020Quote: ... Chloroform extracted RNA was treated with RNase free recombinant DNase I (047 716 728 001, Roche) for 1 h at 37°C using 2 units/μg of RNA ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Neuroscience 2023Quote: ... The embryos were incubated in 50 μL of recombinant Proteinase K (Roche Holding AG, Basel, Switzerland) diluted to ∼2 mg/mL in 1 x tris-EDTA (TE ...
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bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
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bioRxiv - Genomics 2020Quote: ... We used capture enrichment methods for either Illumina or PacBio sequencing based on the use of biotinylated probes to capture Wolbachia DNA (probes designed by Roche NimbleGen) based on 25 complete or draft sequences as described by Lefoulon et al ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...