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Citations for Roche :
551 - 600 of 5646 citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10; 1:50 dilution, Roche). The sections were rinsed in PBS before incubation for 30 minutes with secondary Immunotech biotinylated antibody and 45 minutes with the Streptavidine-Peroxidase Complex (universal HRP immunostaining ...
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bioRxiv - Neuroscience 2022Quote: ... rat colon was homogenized in lysis buffer using 1 mm glass beads (Thistle Scientific) in a MagnaLyser system (Roche). The samples were filtered through a binding column ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... UK). Rat anti-HA mAb (3F10) and rabbit anti-GFP pAb (code No. 598) were from Roche (Basel, Switzerland) and Medical and Biological Laboratories (Aichi ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Cell Biology 2020Quote: ... 3 mM phenylmethanesulphonylfluoride (PMSF) and complete Mini protease inhibitor cocktail (Roche). Following lysis ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-GCTTGAGGTAGC CCTGTTGTCACC-3’ using KAPA HiFi Hotstart polymerase (Roche:KK2602). This PCR reaction produced a 185 bp wild type band and a 750 bp knockout band in heterozygous animals ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... then equilibrated for 3 min in detection buffer (Roche, Catalog# 11585762001). Signals were visualized with CDP-Star Ready-to-Use (Roche ...
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bioRxiv - Microbiology 2020Quote: ... and BCIP (1,5-bromo-4-chlooro-3-indolyl-phosphate, Roche, Switzerland).
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bioRxiv - Developmental Biology 2022Quote: ... and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche). Images were acquired by the Leica MZ10F microscope with the DS-Ri1 camera.
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bioRxiv - Physiology 2022Quote: ... pH 7.4) containing 3 mg/mL collagenase A (Roche Diagnostics, Germany). Oocytes of stage IV and V were manually defolliculated and each oocyte was injected with 50-100 ng of mRNA encoding for the respective photoreceptor CNG channels ...
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bioRxiv - Immunology 2019Quote: ... After blocking for 1 hour in 3% Bovine Serum Albumin (Roche) in TBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... and was subsequently digested with 3 mg/mL Collagenase/Dispase (Roche) in PBS (1X ...
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bioRxiv - Neuroscience 2023Quote: ... with 3 mg/mL DNAse I grade II (Roche, Cat# 104159). The cell suspension was centrifuged at 1,300 rpm for 5 minutes with 7.5% BSA solution (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... and BCIP (5-bromo-4-chloro-3-indolyl-phosphate, Roche 11383221001). Larvae were mounted in 100% glycerol under coverslips and bright-field images captured using a Leica DFC500 camera mounted on a Zeiss Axioskop
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bioRxiv - Biochemistry 2023Quote: ... and 3 cOmpleteTM mini EDTA-free Protease Inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Fat was digested enzymatically with 3 mg/ml collagenase/dispase (Roche) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with 3 U PNGase F (Roche diagnostics) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were injected with diazepam (3 mg/kg, Roche Pharmaceuticals, CH), gaboxadol (10 mg/kg ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mM CaCl2 and protease inhibitor cocktail (CompleteTM EDTA-free, Roche), and homogenized by 10 strokes using Dounce device ...
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bioRxiv - Immunology 2024Quote: ... in HEPES buffer containing Liberase Blendzyme 3 (70 mg/ml; Roche) and DNaseI (30 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Roche) was performed following manufacturer’s indications ...
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bioRxiv - Developmental Biology 2024Quote: ... 5’-AGCGTTCACATCATATGGCA-3’) using Taq DNA polymerase (Roche, Cat. No. 11146165001). Fragments of foxl2l and id1 were cloned into pGEMT-easy plasmid by TA cloning while nanos2 fragment was cloned into pCS2+ plasmid by BamHI and KpnI ...
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bioRxiv - Neuroscience 2024Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP; 11383221001, Roche) was performed in alkaline phosphatase reaction buffer [100 mM Tris pH 9.5 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... The adult inner ears were first were decalcified with 120 mM EDTA for 2 days at 4°C until they were soft and ready for micro-dissecting out the cochlear sensory epithelium for use in whole-amount preparation and immunostaining with the following first antibodies: anti-HA (rat, 1:200, 11867423001, Roche), anti-Prestin (goat ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Slides were incubated at room temperature in the following primary antibodies at 1:500 dilution in 1% BSA/1xPBS: rat anti-HA (Roche) and rabbit anti-αTubulin (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... Denatured and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis using the following antibodies: rat monoclonal anti-HA (11867423001; Roche), mouse monoclonal anti-V5 (V8012 ...
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bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against rat anti-HA clone 3F10 (Roche) or rabbit anti-TgSKP1 UOK75 [19]
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bioRxiv - Biochemistry 2019Quote: ... The antibodies used for the brain-ring gland complex included a rat anti-HA high-affinity monoclonal antibody (3F10, 1:20 dilution; Roche), a guinea pig anti-Shroud antibody (67 ...
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bioRxiv - Cell Biology 2021Quote: ... Retinas from adult Long Evans rats were dissected in DMEM/F12 media supplemented with complete protease inhibitors (Roche, Indianapolis, IN) and positioned above a disc of nitrocellulose disc oriented photoreceptor-side down ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2024Quote: ... BSA in TBST (TBSTB) and probed with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche) diluted 1:1000 in TBSTB for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Immunology 2023Quote: ... only donors were included that had no history of COVID-19 as well as had tested negative in three serological assays (EuroImmun-Anti-SARS-CoV-2 ELISA IgG /S1/, Siemens Healthineers SARS-CoV-2 IgG /RBD/, and Roche Elecsys Ig /Nucleocapsid Pan Ig/). PBMC were selected from heparinized full blood by a standard density gradient (Pancoll Separating Solution ...
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bioRxiv - Genomics 2020Quote: ... Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) were used for the colorimetric detection of alkaline phosphatase activity ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 and 6 post transfection using WST-1 dye (Roche, Basel, Switzerland) as previously described 17.
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bioRxiv - Cell Biology 2019Quote: ... 100 μM ATP and supplemented with 1× phosphatase inhibitors cocktail 3 (Roche). Reactions were carried out at 30°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Neuroscience 2020Quote: ... Following resuspension in (3 ml) homogenization medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...