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Citations for Roche :
701 - 750 of 1044 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... individual primary tumors were dissected from the lungs of KL mice, mechanically dissociated, then digested for 45min in digestion media (10% FBS, pen/strep, 1mg/mLCollagenase/Dispase (Roche) in DMEM ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 30 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Physiology 2023Quote: ... and then incubated for 45 min at 37°C either in Hanks’ Balanced Salt solution (HBSS) or plain DMEM media supplemented with Liberase HD (200 μg/ml, Roche) and DNase I (40 U/ml ...
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bioRxiv - Immunology 2023Quote: ... in a plate and mechanically disrupted using the back-end of a syringe before addition of 50 μL of a digestion media (dRPMI = naRPMI supplemented with 11x collagenase D (#11088866001, Roche, Woerden ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... were used to inoculate each well of a 96-well plate containing 196 μL of LB media (supplemented with ampicillin, and 200uM IPTG) The plate was covered with transparent LightCycler480 Sealing Foil (Roche), and grown for 24 h in a Biotek Synergy H1 plate reader at 42 °C with double orbital shaking ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Bioengineering 2021Quote: ... The blood glucose levels of ZDF rats in each group were measured using a digital glucose meter and test strips (Accu-Chek® Sensor, Roche Inc., Mannheim, Germany) 2 or 3 days prior to surgery and at the study endpoint ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
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bioRxiv - Developmental Biology 2020Quote: Ovaries and testis were fixed and stained according to standard procedures with the following primary antibodies: rat anti-HA (1:500, Roche cat# 11867423001, RRID: AB_390919), rat anti-Vasa (1:100 ...
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bioRxiv - Microbiology 2020Quote: Impedance-based assays were performed using the xCELLigence system (Roche Applied Science and ACEA Biosciences) as described previously (Prasad et al. ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed in radioimmunoprecipitation assay buffer containing complete protease inhibitor cocktail (Roche, Basel, Switzerland) on ice and ultracentrifuged ...
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bioRxiv - Immunology 2022Quote: ... We performed the rhAMP assays in triplicate using a LightCycler 96 Instrument (Roche Molecular Systems) in the endpoint analysis mode.
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bioRxiv - Molecular Biology 2020Quote: ... The HBV DNA was amplified in a real-time PCR assay using LightCycler 480 (Roche) as previously described55 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TUNEL assay was performed using an in situ cell death detection kit (Roche, #11684795910).
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bioRxiv - Genomics 2020Quote: ... Then WST-1 assay was performed by adding WST-1 reagent (cell proliferation reagent, Roche) directly to the culture wells ...
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bioRxiv - Immunology 2021Quote: ... and the enzymatic activity was evaluated by chemiluminescence (β-Gal reporter gene assay, Roche Diagnostics). Anti-CD4 neutralizing mAb L3T4 was used as a control for the blockage of cell fusion (5 µg/mL was preincubated in HeLa-P5 cells for 30 min at 37°C before co-culture with Env+-HeLa cells).
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bioRxiv - Bioengineering 2021Quote: TUNEL assay (In Situ Cell Death Detection Kit, TMR-red, Roche Biochemical Reagents, Waltham, MA) was performed according to the manufacturer's specifications to stain the apoptotic cells.[5] Images were captured at 20X magnification and merged using a Keyence BZ9000 microscope ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μL of luciferin:luciferase reagent (ATP bioluminescence assay kit HSII; Roche Applied Science, Penzberg, Germany) was injected into each well and luminescence was recorded following a 5 second orbital mix and sample measurement at 7 seconds ...
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bioRxiv - Plant Biology 2020Quote: ... The HRM assay was performed with LightCycler® 480 System II (Roche Life Science, Germany). The HRM data was analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Glucose was measured in the supernatant using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche), for calculation of starch content in glucose equivalents ...
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bioRxiv - Cell Biology 2022Quote: ... Intron-spanning primers were designed using the Universal Probe Library (UPL) Assay design centre (Roche). Primer sequences and UPL probes were total SNCA F-tgggcaagaatgaagaaggagc ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with a cocktail of protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular ATP levels were then determined using an ATP Bioluminescence Assay Kit HS II (Roche). Cells were diluted to OD600 = 0.1 with dilution buffer from the kit ...
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bioRxiv - Genomics 2020Quote: ... SYBR Green assay was performed on Light Cycler 480 instrument (2x Probe Master Mix, Roche). All primer sequences are listed in Table S11 ...
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bioRxiv - Microbiology 2022Quote: ... (2) the quantitative Roche Spike Elecsys Anti-SARS-Cov-2 S assay (Roche, IND, USA), which measures spike total antibody concentrations ...
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bioRxiv - Developmental Biology 2022Quote: TUNEL assays were performed using TM red In Situ Cell Death Detection Kit (#12156792910, Roche). Samples were fixed in 4% PFA in FSW for 90 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... telomeric DNA was detected by Southern blotting using TeloTAGGG Telomere Length Assay kit (Roche, 12209136001) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH) (Roche; Cat. Nr.: 11644793001) after 4 hours of incubation at 37°C.
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using a WST-1 cell proliferation assay (Roche, Nonnenwald, Penzberg, Germany). See Supplementary information for details.
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bioRxiv - Developmental Biology 2019Quote: ... Cell proliferation was assessed using both BrDU labeling assay and detection kit (Roche Ref#11296736001) in live cells and with MTT assay kit (Catalog# Sigma CGD1 ...
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bioRxiv - Cell Biology 2019Quote: The proliferative capacity of cells was measured using a BrdU cell proliferation assay (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: Apoptosis was determined by terminal deoxynucleotidyl nick-end labeling (TUNEL) per assay manufacturer’s protocol (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing cOmplete EDTA-free protease inhibitor (Roche) and 10U/ml benzonase nuclease (Santa Cruz) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR assays were performed with SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat# KK4602), using primers indicated in Supplementary Table 2 ...
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bioRxiv - Biochemistry 2022Quote: ... and intracellular ATP was measured using the ATP Bioluminescent Assay Kit HS II (Roche, Switzerland). Cell samples were prepared by diluting treated cells to a final concentration of 3.7×109 cells·mL-1 in 500 μl with a buffer containing 100 mM Tris-HCl pH 7.8 and 4 mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH) (Roche; cat. no. 11644793001) after 4 h incubation at 37°C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche, Switzerland) with four quantification standards included.
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bioRxiv - Neuroscience 2023Quote: ... The free fatty acids (FFA) levels were determined using a colorimetric assay (Roche, Mannheim, Germany). Cholesterol was determined by colorimetric assay (Erba Lachema ...
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bioRxiv - Microbiology 2023Quote: ... qPCR assay was prepared using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems, MA, USA) and performed on an Applied Biosystems ViiA™ 7 Real-Time PCR System according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL assay was performed using the In-Situ Cell Death Detection Kit (TMR Red) (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Anti-nucleocapsid IgG was measured using the Elecsys Anti-SARS-COV-2 assay (Roche; 09203095190) run on a Cobas e411 analyser (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined using assays designed with the Universal Probe Library from Roche (www.universalprobelibrary.com). For each qPCR assay ...
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bioRxiv - Plant Biology 2023Quote: ... and the released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche). For mature grains ...
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bioRxiv - Genetics 2023Quote: ... The RT-qPCR assay was carried out in a LightCycler480 Real Time PCR system (Roche) using the TaqMan gene expression assay ...