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Citations for Roche :
401 - 450 of 699 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Cells were transfected with the appropriate plasmids using Fugene 6 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: U2OS cells were transfected with the indicated plasmids using XtremeGene360 (Roche). When specified ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid transfections were conducted using X-tremeGene DNA Transfection Reagent (Roche) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmids were transfected using X-tremeGENE Hp DNA Transfection Reagent (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with plasmid DNA using X-tremeGene HP (Roche) transfection reagent ...
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bioRxiv - Bioengineering 2024Quote: ... 500 ng plasmid and 1 μL of X-tremeGENE HP (Roche) were diluted in 20 μL Opti-MEM sequentially ...
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bioRxiv - Zoology 2024Quote: ... or the Genopure Plasmid Midi kit (Roche Diagnostics GmbH, Mannheim, Germany). The prepared plasmid was dissolved in TE buffer to 5 µg/µL and stored at −20°C until use.
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated for o/n with 40μl of anti-HA agarose beads (rat anti-HA, 3F10 Roche) at 4 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Molecular Biology 2021Quote: In situ antibody stainings were done as described previously 31 using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-FLAG (M2 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with horseradish peroxidase (HRP)-conjugated rat anti-HA (clone 3F10) monoclonal antibodies (Roche, Indianapolis, IN) at a dilution of 1:5,000 for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: GSDMB2-HA expression was analyzed by immunohistochemistry in 5µm-thick tissue sections using rat anti-HA (1:200; 3F10, ROCHE) or mouse monoclonal anti-GSDMB (1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were immunoblotted for 1 hr at 22°C using the following antibodies: rat anti-HA (1:3000; Roche), mouse anti-V5 (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then blocked for an hour in 5% milk powder/PBS solution and probed with 1:2,500 rat anti-HA mAb 3F10 antibody (Roche) in 5% milk powder/PBS solution overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% milk and probed overnight at 4°C with 1:2,500 rat anti-HA mAb 3F10 (Roche). They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare) ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... The membrane was blotted overnight at 4°C with rat antibody against the HA tag (Roche, 1/500 dilution), TRAF2 and TRAF6 rabbit antibodies (Suppl ...
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bioRxiv - Neuroscience 2019Quote: ... some TK rats (n=11) were orally administered 4 mg of valganciclovir (val) to reduce neurogenesis (Hoffman La-Roche; delivered in 0.5 g peanut butter + chow pellets ...
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bioRxiv - Cell Biology 2019Quote: ... and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10; 1:50 dilution, Roche). The sections were rinsed in PBS before incubation for 30 minutes with secondary Immunotech biotinylated antibody and 45 minutes with the Streptavidine-Peroxidase Complex (universal HRP immunostaining ...
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bioRxiv - Neuroscience 2022Quote: ... rat colon was homogenized in lysis buffer using 1 mm glass beads (Thistle Scientific) in a MagnaLyser system (Roche). The samples were filtered through a binding column ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... UK). Rat anti-HA mAb (3F10) and rabbit anti-GFP pAb (code No. 598) were from Roche (Basel, Switzerland) and Medical and Biological Laboratories (Aichi ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Developmental Biology 2020Quote: ... Paired guide-RNA plasmids were transfected into ESCs using FUGENE 6 (Roche) transfection reagent following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Both plasmids were co-transfected in HDF using X-tremeGENE HP (Roche) as described for PA-TagRFP-emerin ...
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bioRxiv - Genetics 2019Quote: ... coli strains using the High Pure plasmid isolation kit (Roche, Basel, Switzerland). PCR amplification of short DNA fragments (<3000 bp in length ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were purified with a Genopure Maxi kit (Roche, Basel, Switzerland) and the final concentration was 2 µg/µl.
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bioRxiv - Neuroscience 2022Quote: ... from the linearized plasmids with DIG or FITC RNA labeling mix (Roche), respectively.
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bioRxiv - Cell Biology 2022Quote: ... The plasmid was transfected into H1 hESCs with XtremeGene 9 (Roche, 6365787001), and 24 h later 5000 GFP+ cells were sorted into a well of six-well plate using mTeSR1 medium supplemented with 10 µM Y-27632 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with various plasmids in duplicate using XtremeGENE (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells at ∼40% density were transfected with plasmids using Fugene HD (Roche). The transfected cells were cultured for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: Plasmid transfections were performed using X-tremeGENE 9 DNA transfection agent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Addgene plasmid #18003) followed by selection with 0.4 mg/ml G418 (Roche). All cell lines were grown in culture media recommended by the suppliers and were maintained in humidified incubators at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Plasmid transfections were performed using X-tremeGENE HP DNA Transfection Reagent (Roche). For RNA interference ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were transfected using X-tremeGENE 9 DNA transfection reagent (Roche, 6365787001) following the manufacturer’s protocol (1 μg plasmid ...
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bioRxiv - Physiology 2022Quote: ... 150 ng of each plasmid were transfected with X-tremeGENE 9 (Roche) in C2C12 myoblasts immediately after trypsinization (47) ...
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bioRxiv - Immunology 2023Quote: ... and 0.8 µg of transfer plasmid using 5.8 X-tremeGENE HP (Roche). Media was replaced after 16 hours and supernatant harvested after a further 24 hours by filtering through a 0.45 µm cellulose acetate filter ...
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bioRxiv - Cell Biology 2023Quote: U2OS cells were transfected with the indicated plasmids with Fugene 6 (Roche) according to manufacturer’s instructions using a 3:1 Fugene/plasmid ratio ...
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bioRxiv - Immunology 2023Quote: ... and ligated to gel-purified plasmid backbones using T4 DNA ligase (Roche). Competent DH5a E ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection of plasmid DNA was performed using X-tremeGENE™ HP (Roche) and siRNAs were transfected using RNAiMAX (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... and 0.8 µg of transfer plasmid using 5.8 X-tremeGENE HP (Roche). Media was replaced after 16 hours and supernatant harvested after a further 24 hours by filtering through a 0.45 cellulose acetate filter ...
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bioRxiv - Genomics 2024Quote: ... in vitro synthesized from a linearized plasmid using RNA Labeling Mix (Roche) and T3 RNA polymerase (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... The adult inner ears were first were decalcified with 120 mM EDTA for 2 days at 4°C until they were soft and ready for micro-dissecting out the cochlear sensory epithelium for use in whole-amount preparation and immunostaining with the following first antibodies: anti-HA (rat, 1:200, 11867423001, Roche), anti-Prestin (goat ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Slides were incubated at room temperature in the following primary antibodies at 1:500 dilution in 1% BSA/1xPBS: rat anti-HA (Roche) and rabbit anti-αTubulin (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... Denatured and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis using the following antibodies: rat monoclonal anti-HA (11867423001; Roche), mouse monoclonal anti-V5 (V8012 ...