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Citations for Roche :
601 - 650 of 939 citations for RNase Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Cell death was determined by lactate dehydrogenase (LDH) activity using a Cytotoxicity Detection Kit (LDH) (Roche). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... MAD2L1 was detected by RT-qPCR analysis using the SYBR Green detection system (Roche Applied Science) and normalized to the level of the GAPDH mRNA expression and using the 2-ΔΔCT cycle.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with In Situ Cell Death Detection (TUNEL) Kit with TMR Red (Roche, 12156792910) according to the vendor’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Immunology 2023Quote: ... ADCC was measured using the LDH release assay (Cytotoxicity Detection Kit (LDH) (Roche; cat. no. 11644793001) after 4 h incubation at 37°C.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected using an anti-rabbit HQ detection system (#7017936001 and 7017812001, Roche Diagnostics) followed by Chromomap DAB IHC detection kit (#5266645001 ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were processed using the In Situ Cell Death Detection Kits (Roche Life Science) according to manufacturer’s instruction.
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bioRxiv - Biochemistry 2023Quote: ... Probe preparation and subsequent DIG detection were conducted using the DIG Northern Starter Kit (Roche, 12039672910) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... . Apical wash and basolateral samples were assayed following protocol instructions (Cytotoxic Detection Kit, Roche Applied Science). To calculate absolute values ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by chemiluminescent detection on a Fusion FX7 Edge (Vilber) using Lumi-Light Plus Substrate (Roche). The membrane was then stripped with Restore PLUS Western Blot Stripping Buffer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysis buffer was supplemented either with 40 U/ml RNase inhibitor (Molox) or 50 U/ml Benzonase (Roche) to differentiate between RNA- and protein-dependent interactions ...
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bioRxiv - Genomics 2020Quote: ... the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl Protector RNase Inhibitor, ROCHE) and filtered through a 30μm cell strainer (Fisher Scientific) ...
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bioRxiv - Physiology 2019Quote: ... 2.5 µl of cDNA in RNase free water was made up to 25 µl with FastStart Universal SYBR Green Master (ROX, 12.5 µl, Roche), Ultra Pure Water (8 µl ...
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bioRxiv - Microbiology 2019Quote: ... The solution was then supplemented with 20 mM MgSO4 and treated with 50 μg mL-1 RNase A (Roche) and 10 μg mL-1 DNase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 1μL DTT (0.1 M) and 0,5 μL of protector rnase inhibitor (40 U/μL, Hoffmann-La Roche, Basel, Switzerland) were added and the mix incubated at 37° C per 2 min ...
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bioRxiv - Neuroscience 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). FRMP binding assay was performed by incubating biotinylated transcripts (250 ng ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were incubated for 30 min at 37°C with 20 U of RNase-free DNase I (Roche) in a final reaction volume of 30 μl ...
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bioRxiv - Pathology 2022Quote: ... 2.5 μl of cDNA in RNase free water was made up to 25 μl with FastStart Universal SYBR Green Master (ROX, 12.5 μl, Roche), Ultra Pure Water (8 μl ...
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bioRxiv - Developmental Biology 2022Quote: ... Precipitated RNA was resuspended in RNase- and DNase-free water and DNA digestion was performed using DNase I (Roche) with 2U/ul of RNasin® Plus (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... Crosslinks were reversed at 65°C overnight and the eluates were treated with RNase A and Proteinase K (Roche), prior to an extraction with phenol:chloroform:isoamyl alcohol ...
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were pooled by stage and underwent three rounds of centrifugation at 1500rcf at 4°C for 10 minutes followed by rehydration with DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was digested on column with the addition of an extra 1μl of RNase-free DNase I (Roche) to ensure complete digestion ...
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bioRxiv - Cell Biology 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Neuroscience 2020Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Developmental Biology 2022Quote: ... samples from the same stage were pooled and resuspended in DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and pellets were re-suspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL) (Roche). Nuclei were incubated with fluorescently conjugated antibodies against the neuronal marker NEUN (RBFOX3/NEUN (1B7 ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Immunology 2023Quote: ... The DNA samples were treated with 0.5 μg/μl of RNase A for 30 min at 37°C and then with 0.25 μg/μl of Proteinase K (Roche) in the presence of 10mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped by adding one volume of 2X STOP Buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 50ug/mL RNase A (Cat #3335399001, Roche), 50ug/mL Linear Acrylamide (Cat #K548 ...
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bioRxiv - Plant Biology 2024Quote: ... the tissue was finely chopped on ice for approximately 1 minute using 600 μL of pre-chilled Nuclei Isolation Buffer containing 0.4U/μL RNase inhibitor (Roche, Protector RNase Inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... TUNEL staining was performed according to manufacturer’s instructions (In situ cell death detection kit TMR red, Roche). For the positive control ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL was performed as described in [68] using In Situ Cell Death Detection Kit provided by Roche Diagnostics ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated in HNPP/Fast red working solution according to manufacturer’s instructions (HNPP Fluorescent Detection Set, Roche). To stop the reaction ...
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bioRxiv - Genetics 2019Quote: Detection of tRNA precursors was performed as described (Otsubo et al., 2018) using Digoxigenin labeled probes (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... as well as Southern blot using DIG-High Prime DNA labeling and Detection starter kit II (Roche to verify only single insertion of the NAT cassette into each of the strains (577bp) ...
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bioRxiv - Genomics 2021Quote: Lactate dehydrogenase (LDH) release in the apical medium was measured with the Cytotoxicity Detection Kit (Roche 04744926001) as described previously (36) ...
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bioRxiv - Bioengineering 2021Quote: Cell membrane integrity was measured via the lactate dehydrogenase activity (LDH) release assay (Roche Cytotoxicity Detection Kit). Media from the bottom channel was continuously collected in 15 minutes intervals (both for the unstretched and stretched mimicking VILI) ...
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bioRxiv - Plant Biology 2021Quote: ... For post-hybridization and signal detection the procedure mentioned in the kit was followed (Roche Diagnostics, Germany).
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bioRxiv - Microbiology 2019Quote: ... were performed using the DIG High Prime DNA Labelling and Detection Starter Kit I (Roche, Basel, Switzerland). HindIII produces 8 kb ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL stainings were performed as IF experiments with the TMRRed In situ dell death detection kit (Roche).
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using an anti-rabbit HQ detection system (catalog # 7017936001 and 7017812001, Roche Diagnostics) followed by Chromomap DAB IHC detection kit (catalog # 5266645001 ...
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bioRxiv - Cancer Biology 2020Quote: ... All the cells in culture were negative on periodically testing for mycoplasma using Mycoplasma Detection Kit (Roche). All the human and mouse cells ...
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bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
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bioRxiv - Genetics 2022Quote: ... Southern blotting was performed using DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and DIG Wash and Block Buffer Set (Roche 11585762001 ...