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Citations for Roche :
151 - 200 of 203 citations for Pyr6 Substance P since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Protein from cell pellets was extracted with 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], and 150mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: 1×106 cells were lysed in 50 ul NP40 lysis buffer (0.5% Nonidet P-40, 50 mM Tris, pH 7.8, 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 200 mM NaCl; 1mM EDTA, pH 8.0; 0.5 % Nonidet P-40; 1 tablet complete protease inhibitor per 50ml, Roche, Mannheim, Germany). For each pulldown ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP-40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cancer Biology 2020Quote: ... washed with 1X PBS and respuspended in 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: Protein was harvested from cell pellets lyzed using 2x pellet volume NP-40 lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], and 150mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... The skin was finely minced with a scalpel and placed for 30 min at 37 °C on a shaker in a digesting enzyme cocktail of 2 mg/ml Collagenase P (Roche), 2 mg/ml Dispase (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2022Quote: ... pancreas was perfused through the common bile duct with 2 mL of 0.8 mg/mL collagenase P (Roche, Indianapolis, IN) in Hanks’ balanced salt solution [HBSS] with Ca2+ and Mg2+ (Corning ...
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bioRxiv - Genetics 2022Quote: ... Expression patterns were visualized with a Nitro-Blue Tetrazolium Chloride/5-Bromo-4-Chloro-3-Indolyphosphate p-Toluidine Salt (NBT/BCIP) system (Roche). Sections were mounted with Vectamount (Vector laboratories ...
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bioRxiv - Plant Biology 2019Quote: ... 60 mM KOAc, 10 mM MgOAc, 0,5% [v/v] Nonidet P-40 and proteinase inhibitor cocktail [cOmplete™, COEDTAF-RO, Roche]). After a solubilization step (15 min ...
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bioRxiv - Cancer Biology 2019Quote: Pancreatic tumors were resected from the mice and mechanically and enzymatically digested in Hanks Balanced Salt Solution (HBSS) with 1 mg/mL Collagenase P (Roche) Cell suspensions were centrifuged for 5 min at 1500 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... and then incubated at room temperature in the same solution containing NBT-BCIP (nitrotetrazolium blue chloride at 350 µg/ml and 5-bromo-4-chloro-3-indolyl phosphate p-toluidine salt at 175 µg/ml) (Roche) until the color appeared ...
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bioRxiv - Microbiology 2020Quote: ... 24 hours post infection cells lysed using NP-40 buffer (50 mM Tris, 150 mM NaCl, 1% Nonidet P-40, and Complete Mini protease inhibitor cocktail [Roche]). Insoluble material were removed by centrifugation ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 cells were lysed in 50 μl NP40 lysis buffer (0.5% Nonidet P-40, 50 mM Tris, pH 7.8, 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation was performed using IP buffer (1% Nonidet P-40, 50mM Tris-HCl [pH 7.5], 150mM NaCl, and Complete™ protease inhibitor cocktail-EDTA (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... for five minutes at room temperature before being incubated in the dark with 2% Nitro-Blue Tetrazolium Chloride/5-Bromo-4-Chloro-3⍰-lndolyphosphate p-Toluidine Salt (NBT/BCIP; Roche) in buffer 3 at room temperature until staining developed ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP-40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)) for 10 minutes at 4°C and spun at 1350g for 5 minutes at 4°C to isolate nuclei ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cancer Biology 2020Quote: ... minced with a sterile scalpel in a petri-dish containing complete medium (DMEM/10%FBS/2mM L-Glutamine/1x NEAA/1x Sodium Bicarbonate/10mM HEPES/ 1x Pen/Strep) and then incubated with complete medium containing 1 mg/ml Collagenase P (Roche) for 90 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: Protein from cell pellets was extracted with 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], and 150mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with 1X PBS and resuspended in 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50 mM Tris [pH 7.8], 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and resuspended in 2x pellet volume NP40 protein lysis buffer (0.5% Nonidet P-40, 50 mM Tris [pH 7.8], 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (MilliporeSigma) ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 mM AEBSF and 1x Halt protease inhibitor cocktail from ThermoFischer) and incubated for 30 min on ice before addition of Nonidet P 40 substitute (Roche) to a final concentration of 0.5% ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Developmental Biology 2023Quote: ... 250 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Nonidet P-40 and protease inhibitor cocktail from Roche, France), were then added to the mix and gently rotated at 4°C for 30 min ...
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bioRxiv - Physiology 2024Quote: ... pancreas was distended by cannulizing the bile duct to infuse 2.5ml of collagenase P (0.66mg/ml in Hank’s Balanced Salt Solution) (Roche, catalog number C7657), surgically removed and incubated at 37°C for 15 minutes under constant agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... washed with 1X PBS and resuspended in 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50 mM Tris [pH 7.8], 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... transient transfection was performed with a total of 1 µg of plasmids (EGFRmCitrine, PTBmCherry and cCblBF P at ratio 4:3:4 by mass) using FUGENE6 (Roche Diagnostics) transfection reagent and Opti-MEM (Gibco - Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The adherent cells were lysed with ASM lysis buffer (250 mM sodium acetate, pH 5.0, 0.1% Nonidet P-40, 1.3 mM EDTA, and 1x Roche Complete protease inhibitor cocktail) for at least 15 min and were scraped off with a pipette tip and collected ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris/HCl pH7.5, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.1% [v/v] Nonidet P-40/Igepal, Roche cOmpleteTM protease inhibitor) was added to grinded-frozen samples ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were suspended in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1x Roche protease inhibitor mixture) and sonicated 5 times x 5 min (30 s ON/ 30 s OFF ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100, 0.2% Nonidet P-40, 0.6 mM PMSF, and 20 µM MG132 in a Roche protease inhibitor cocktail) was added ...
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bioRxiv - Biophysics 2019Quote: ... was assessed by following its amidolytic activity toward the chromogenic substrate Tos-Gly-Pro-Arg-p-nitroanilide (Chromozym TH, Roche, Amadora, Portugal) at 37°C in the presence of 0.40 nM of a synthetic variant of an anticoagulant produced by D ...
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bioRxiv - Biochemistry 2022Quote: Mice were euthanized by cervical dislocation and subjected to bile duct cannulation and digestion of the pancreas with a mixture of collagenase P (11-249-002-001, Roche, Basel, Switzerland), hyaluronidase (H3506 ...
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bioRxiv - Physiology 2021Quote: Pancreases were rapidly dissected after intra-ductal injection from 9-10 week-old male mice and islets were isolated by digesting the pancreas with collagenase P (Roche, Basel, Switzerland) and purified using a Ficoll gradient (Merck Biochrom ...
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bioRxiv - Bioengineering 2023Quote: ... 10-12 DRGs per donor were disintegrated into micro tissue units using 6 mL collagenase P (4 mg/mL, Roche, Mannheim, DE) in a 15 mL Falcon tube on an orbital shaker for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 0.1 mM EGTA, 100 mM NaCl, 1% Nonidet P-40, 10 mM Imidazole, supplemented with Roche protease inhibitor cocktail). Cell lysates were clarified by centrifugation at 10,000× g for 5 min at 4 °C and then incubated at 4 °C under constant rotation with His-ΔFIP1c-RCP559-649 fusion protein ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of repair plasmid and 190 ng of pIB_Cas9_Blast (encoding SpCas9 and carrying Blasticidin resistance, kind gift of P. Heun) using X-tremeGENE HP DNA Transfection Reagent (Roche, Cat. No 6366236001). 24 hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche; P/N: KK4873) on the QuantStudio 12K device to quantify the library ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 250 mg of powder was thawed at 4°C followed by resuspension in 1 mL of HIP buffer (40 mM HEPES-KOH pH 7.5, 110 mM KOAc, 2 mM MgCl2, 1% Triton X-100, 0.1% Tween, 1x protease inhibitor cocktail [Roche], 1% solution P, 1 mM DTT). The lysate was next passed through a Whatman 25 mm GD/X Disposable filter (Cat No ...
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bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...