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Citations for Roche :
251 - 300 of 5543 citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1 mL digestion mix (4.8 mg/ml Collagenase IV; Worthington Biochem, and 200 μg/ml DNase I; Roche Diagnostics, in 1x PBS) was added after which the tissue was incubated at 37°C for 45 min ...
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bioRxiv - Biochemistry 2022Quote: Mice were euthanized by cervical dislocation and subjected to bile duct cannulation and digestion of the pancreas with a mixture of collagenase P (11-249-002-001, Roche, Basel, Switzerland), hyaluronidase (H3506 ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse MEC organoids were prepared by manual chopping of mouse mammary gland tissues harvested from 10–12-week-old mice followed by digestion with shaking for 1 hr at 37 °C with Collagenase A from Clostridium histolyticum (10 mg/mL; Roche, Cat. #: 13560925) and Hyaluronidase from bovine testes (1 mg/mL ...
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bioRxiv - Immunology 2021Quote: Adipose and other tissues were removed from isolated thymi which were subsequently subjected to three rounds of enzymatic digestion for which cut up lobes were incubation at 37°C in Liberase digestion buffer (PBS Gibco 1X, Liberase Roche 2.5 mg/ml, DNase Roche 10 mg/ml). Cells were incubated with anti-CD45 beads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... and processed in digestion buffer (1 mg/ml of Collagenase D and 80 U/ml DNase I, both from Roche, in DMEM) and a GentleMACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2023Quote: ... the plasmid DNA was linearized by SalI digestion and transcribed by T7 RNA polymerase using Digoxigenin (Dig) RNA Labeling Mix (Roche Diagnostics, Germany) for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
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bioRxiv - Physiology 2024Quote: Coarsely chopped liver fragments from each mouse were digested in 5 ml liberase-based digestion mix (5 ml of DMEM [Thermo Fisher Scientific, Cat #61965059] + 1 mg Liberase™ Research Grade [Roche, Cat #5401127001] + 0.1 μg DNAse I [Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Approximately 100 mg of minced PND12 rat ovary tissue was digested in 1ml of digestion medium [199 media containing 0.08 mg/ml of liberase with medium-concentration of thermolysin (Roche Diagnostics GmbH, Mannheim, Germany) supplemented with 5U/ml of DNase I and 1% BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... followed by mechanical and enzymatic digestion with 150 μL collagenase/dispase (1 mg/mL) and 300 μL DNAse (10 mg/mL; both Roche diagnostics, IN, USA) for 45 min at 37 °C with mild agitation ...
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bioRxiv - Plant Biology 2020Quote: ... and was then cloned into the binary vector pGreen 0229 (Hellens et al., 2000) via restriction digestion with EcoRI and NotI enzymes (Roche Diagnostics, Mannheim, Germany). The AtD14 construct (comprising 553 bp sequence upstream of the start codon and 804 bp of the coding sequence ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Physiology 2020Quote: ... finely cut with scissors and incubated in digestion medium (PBS with 2% BSA, 1M CaCl2, 94U/ml dispase II and 100mg/ml collagenase D, Roche Life Science, France) at 37°C for 30-40min ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was aspirated and the pellet was resuspended in a pre-warmed digestion mix containing 1 mg ml-1collagenase/dispase (Roche Diagnostics, Indianapolis, USA) and 10 μg ml-1DNase I (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G agarose beads (Roche #11719416001) pre-blocked with salmon sperm (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... together with protein A agarose (Roche) overnight at 4 ℃ ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein G agarose beads (11243233001, Roche) were added to the lysates and incubated for additional 4 hours at 4°C with rotary agitation ...
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bioRxiv - Neuroscience 2021Quote: ... homogeneous protein G-agarose suspension (Roche) was added to the suspension ...
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bioRxiv - Microbiology 2019Quote: ... Protein G-conjugated agarose beads (Roche) or PrecipHen for chicken antibodies (Aves Labs ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor cocktail (Roche, Mannheim, Germany) and then sonicated ...
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bioRxiv - Genetics 2021Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... and Protein G-Agarose (Roche, 11243233001) were added to each sample and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Immunology 2023Quote: ... mLNs were incubated at 37°C in digestion medium (RPMI-1640 supplemented with 0.8 mg/mL dispase, 0.2 mg/mL Collagenase P [Roche] and 0.1 mg/mL DNase I [Invitrogen]) and gently pipetted at 5-minute intervals to ensure complete dissociation of the tissue ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...