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Citations for Roche :
651 - 700 of 5543 citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... protein eluates from every purification step were immediately supplemented with protease inhibitors (EDTA-free cOmplete, Roche) (together with DTT at 10 mM for cysteine-containing protein constructs) ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were extracted with ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Physiology 2024Quote: Protein cardiac extracts were obtained by tissue homogenization with ceramic beads (MagNa Lyser Green Beads, Roche) in lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...
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bioRxiv - Cancer Biology 2021Quote: ... 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cancer Biology 2021Quote: ... The exome captured by the SeqCap wash kit and SeqCap pure capture bead kit (both from Roche). The Illumina Novaseq platform was used to sequence 150 bases (paired-end sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Plant Biology 2022Quote: ... The EMSA was carried out utilizing the manufacturer’s instructions (DIG-gel shift kit 2nd generation kit, Roche).
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bioRxiv - Genomics 2019Quote: ... using the DNA 1000 kit and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of Hiseq2500 in single read 100nt mode using the clustering and SBS v3 kit following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were subjected to total RNA extraction using a commercially available kit (High Pure kit, Roche, Indianapolis, IN). Total RNA was then used to make complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...
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bioRxiv - Epidemiology 2019Quote: ... An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems, Wilmington, MA). The amplicon pool was sequenced on the Illumina MiSeq generating paired end 300 bp reads ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using a stand specific kit RNA-seq kit with ribosome depletion (KAPA Biosystems) and NEBNext Adapters and Multiplex Oligos for Illumina ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Cell Biology 2019Quote: ... samples were further incubated for 2 hrs in 40 µl pre-cleared protein-G agarose beads (Roche). Beads with immunocomplexes were centrifuged at 3,000 × g and washed four times in lysis buffer with intermittent incubations ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... and then scraped in 10 ml PBS + 1X Protein Inhibitor Cocktail buffer (catalog no. COUL-RO, Roche) and centrifuged for 5 min at 1,000 x g at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were subjected to 10% SDS-PAGE gels for immunoblot analyses using anti-GFP (Roche) and anti-Myc (Roche ...
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were western blotted onto PVDF membranes and probed with the following antibodies: anti-HA (11867423001, Roche), anti-Flag (F3165 ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Cells were harvested, lysed in RIPA buffer (Cell Signalling, 9806) containing a protein inhibitor cocktail (Roche, 11873580001), and centrifuged at 4°C for 10 min at 12,000 g/min ...
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bioRxiv - Molecular Biology 2020Quote: ... (a) Proteins were extracted from cryogrinded cell powders with different extraction solutions supplemented with protease inhibitors (Roche) (solution compositions listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing GST fusion proteins were resuspended in PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and lysed by lysozyme treatment (1 mg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... and protein was extracted via Dounce homogenizer in a cocktail of RIPA buffer (Thermo) and proteinase (Roche) supplemented with phosphatase (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins from fracture femora were extracted by RIPA buffer combined with protease inhibitors and phosphatase inhibitors (Roche). The supernatants were obtained by centrifugation (12,000g ...
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bioRxiv - Cell Biology 2020Quote: Whole cell or mitochondrial samples were lysed in RIPA buffer supplemented with cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluorid (PMSF) ...
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bioRxiv - Genetics 2020Quote: ... we assessed levels of protein in mice urine using Chemstrip® 10 (Roche Diagnostics GmgH, Mannheim, Germany).
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells using Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X cOmplete Protease InhibitorCocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Biophysics 2022Quote: ... and 500-750 μg lysate was pre-cleared with Protein G agarose beads (Roche; Cat. No.11719416001) for 1 hour at 4°C with rotation at 5 rpm in a rotator ...
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bioRxiv - Plant Biology 2022Quote: ... For every biological replicate 500 μg protein was digested with sequencing grade trypsin (1:100 trypsin:protein; Roche) overnight at room temperature ...