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Citations for Roche :
151 - 200 of 1077 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA capture beads (Kapa Biosystems, Bedford row, London). Each sample was then eluted in 20 μL of elution buffer adjusted to 10 mM ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Cell Biology 2019Quote: For MS analyses immunoprecipitates on Protein-G Dybabeads were washed 2x with TEAB Buffer (100mM) and incubated with Trypsin (Roche) at 37°C for 18 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged for 15 minutes at 13000 rpm and then the supernatants were incubated mouse monoclonal anti-huntingtin antibody MAB2166 (MilliporeSigma) conjugated Protein G Agarose (Roche) at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Plant Biology 2023Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems/Roche), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems/Roche), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... in an IP buffer (1xPBS, 5% glycerol, 0.5 mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were prepared using the IP lysis buffer (0.5% NP40 (Roche, Basel, Switzerland), 1x cOmplete protease inhibitor (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were resuspended in 1.0 ml IP buffer containing protease inhibitor cocktail (Roche), cells were lysed and insoluble matter was removed by centrifugation at 13000 rpm for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... incubation at 4 °C rotating for 3 h, followed by incubation (4 °C, rotating) with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Genetics 2021Quote: ... IP and input DNA levels were quantified by qPCR using SYBR green PCR mastermix (Roche) on a 7300 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were lysed in IP Lysis Buffer supplemented with cOmplete protease inhibitor cocktail (Roche), followed by centrifugation at 15,000 rpm at 4°C ...
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bioRxiv - Physiology 2022Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems / Roche, Basel, Switzerland), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Physiology 2022Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems / Roche, Basel, Switzerland), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems / Roche, Basel, Switzerland), and quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems / Roche, Basel, Switzerland), and quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Treated or untreated transfected HEK cells were lysed in cold IP buffer with protease inhibitors (Roche), and centrifuged for 10 minutes and 5,000 rpm at 4°C ...
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bioRxiv - Physiology 2019Quote: ... and homogenization tubes containing ceramic beads (MagNA Lyser Green Beads, Roche, Germany). Human cells from differentiation experiments in monolayer were also lysed in Tri-Reagent for 5 minutes before being removed from the wells using a sterile cell scraper ...
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bioRxiv - Immunology 2022Quote: ... Agarose beads (Roche, C755B62) was added to the remaining supernatant ...
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bioRxiv - Genetics 2019Quote: ... SeqCap Capture Beads (Roche) were used to recover hybridized cDNA ...
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bioRxiv - Neuroscience 2022Quote: Agarose beads (Roche, 11719416001) were equilibrated in 1X lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Pure Beads (Kapa Biosystems) were used to separate the ds cDNA from the second strand reaction mix resulting in blunt-ended cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... G-418 solution (Roche; 4727878001), L-Leucyl-L-Leucine methyl ester (LLOMe ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested on ice in Pierce IP lysis buffer and EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in IP Lysis Buffer (87787, Pierce™) supplemented with protease inhibitors (Complete mini, Roche), and IP was performed with antibodies against survivin (RnD AF886 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were lysed in 250 μl IP Lysis Buffer (Pierce) supplemented with complete protease inhibitors (Roche) for 30 minutes at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unreacted compound was removed via centrifugation using a molecular weight cutoff filter or G-25 Sephadex Quick Spin Protein columns (Roche Applied Science, Indianapolis, IN).
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bioRxiv - Cell Biology 2022Quote: ... or anti-HA beads (Roche) for 1 hour at 4°C ...
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bioRxiv - Genomics 2020Quote: ... or Kapa beads (Roche Diagnostics). When isoG forward primer was used ...
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bioRxiv - Plant Biology 2023Quote: ... MagNA Lyser Green Beads (Roche) was used for the initial lysis step as described in Shirzadi et al ...
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1 g/L Proteinase K (Roche), 2.5 mM dNTPs (Takara) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 g/L Proteinase K (Roche), 2.5 mM dNTPs (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 g of agarose powder (Roche) was dissolved in 72 mL of Milli-Q H2O ...
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bioRxiv - Plant Biology 2021Quote: Root tissue was ground in IP buffer (50mM Tris-HCL, pH7.5, 150mM NaCL, 10% glycerol, 0.1% NP40) containing cOmplete protease inhibitor (Roche). After clearing lysates at 12,000x g (10 min) ...
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bioRxiv - Cancer Biology 2021Quote: For HA pulldown assays the cells were lysed with 500 µL of IP Lysis Buffer containing cOmplete (Roche) protease inhibitor cocktail and incubated at 4°C for 35 min on a rotator followed by centrifugation at 15000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The nuclear lysates were diluted 2 fold with IP buffer (20mM HEPES, pH 7.9, 0.2M NaCl, complete protease inhibitors (Roche) and then flag-beads (Anti-Flag Magnetic Beads ...
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bioRxiv - Microbiology 2022Quote: ... Cells were combined and lysed in 1 ml co-IP buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 0.5% NP-40, 1X cOmplete protease inhibitor (Roche)) and divided into equivalent fractions for ICC ...
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bioRxiv - Genomics 2019Quote: ... beads were washed 3×1mL with bead wash buffer (1x PBS, 5mg/mL BSA, Roche complete EDTA-free protease inhibitor ...
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bioRxiv - Physiology 2023Quote: Muscle tissue was homogenised in tubes containing ceramic beads (MagNA Lyser Green Beads, Roche, Germany) and 1 ml Tri-Reagent (Invitrogen ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The samples were incubated on ice for 10min and then lysates were diluted 1:2 with CNBr IP wash buffer (50 mM Tris–HCl pH 7.5, 150mM NaCl, 1mM EDTA, 1/50 protease inhibitor tablets [Roche]). The homogenates were incubated on ice for 15min and were cleared by centrifugation at 12,000g for 15min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005 % NP40 and proteinase inhibitors (Roche)) and mixed with 20 μl anti-FLAG magnetic beads (Merck Millipore) ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were washed five times with the IP buffer (10mM Tris pH8.0, 150mM NaCl, 0.01% NP-40, 1mM MgCl2, 1X Protease inhibitor (Roche,11836170001), 5% Glycerol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested 48 hours post transfection and lysed in IP Lysis Buffer (Pierce) supplemented with protease inhibitors (Roche) for 45 minutes at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005% NP40 and proteinase inhibitors (Roche)) and mixed with 20 μl anti-FLAG magnetic beads (Merck Millipore M8823) ...