Labshake search
Citations for Roche :
251 - 300 of 10000+ citations for Progesterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Pathology 2019Quote: ... cDNA synthesis was performed with a 1 μg RNA sample using the First Strand cDNA Synthesis kit RT-PCR (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Molecular Biology 2019Quote: ... For quantitative-RT PCR analysis 1 µg of total RNA of each sample was reversed-transcribed to cDNA (Transcriptor First Strand cDNA Synthesis kit, Roche 04379012001), cDNA was diluted 1:20 and used as template for amplification reactions ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA-seq libraries were then prepared using 1 µg of RNA from each sample and the KAPA RNA HyperPrep Kit with RiboErase (Roche KR1351) according to the manufacturer’s protocol with 10 amplification cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Cell Biology 2023Quote: ... samples were covered with the staining mixture at 37 °C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA of 1 μg from each sample was reverse transcribed with random hexamers using Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with oligo(dT)12-18 using Transcriptor first-strand synthesis kit (Roche, Basel, Switzerland). Transcript levels were measured by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2019Quote: ... qPCR reactions were set up using 96-well plates with FastStart Universal SYBR Green Master (Roche) and 1.5 µM primers (found in Table S2) ...
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bioRxiv - Plant Biology 2020Quote: ... qPCR reactions were conducted on the LightCycler 480 Multiwell Plates 384-well themocycler (Roche Applied Sciences), with the following amplification program ...
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bioRxiv - Plant Biology 2021Quote: ... RT-PCR was performed using 96 well plates in a LightCycler® 480 Instrument by Roche. PCR reactions were performed using KAPA SYBR FAST qPCR Master Mix kit by Kapa Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed in LightCycler 480 96-well plates (Roche Diagnostics, Indianapolis, IN, USA) using a 10 minute pre-incubation period at 95°C followed by 40 cycles of denaturation for 5 seconds at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... PCR array were performed in 384-well plates on a LightCycler 480 instrument (Roche Applied Science). The reaction mix of 102 ul of sample cDNA was prepared using 2x SA Biosciences RT2 qPCR Master Mix and 10 ul of this mixture was added into each well of the PCR array ...
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bioRxiv - Molecular Biology 2023Quote: ... Gene expression was analyzed by quantitative PCR (qPCR) in 384-well plates using the LightCycler480 (Roche). For each tissue ...
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bioRxiv - Microbiology 2023Quote: ... qPCR experiments were performed in a 384-well plate using LightCycler 480 SYBR Green (Roche; #4887352001) in a Roche LightCycler 480 II PCR system utilizing a Roche LightCycler 480 Software v1.5.1.62 ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time quantitative PCR performed in duplicates in 384-well plates with a LightCycler 480 (Roche) using SYBR Green I Master (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Denaturation assays were performed in a 384-well plate in a qPCR machine (Roche Lightcycler II) using a temperature range of 25-99 °C ...
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bioRxiv - Microbiology 2024Quote: ... the master mix was added and the plate was placed in a LightCycler 480 II (Roche). The results were evaluated by Delta delta CT method considering the Cp values obtained from LightCycler 480 Software release 1.5.0 ...
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bioRxiv - Genomics 2022Quote: ... High Sensitivity DNA Analysis Kit and KAPA SYBR FAST qPCR Kit (Roche). WGS was performed on the HiSeq X (HCS HD 3.5.0.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNase free kit (Roche). RNA amount was quantified and cDNA was prepared using TaqMan Reverse Transcription Reagents (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2019Quote: ... The cDNA was diluted 20-fold and used for qRT-PCR employing a LightCycler® 480 SYBR Green 1 Master PCR labelling kit (Roche Applied Sciences) and RotorGene 3000 Real time PCR machine (Corbett Research ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription of 1 µg RNA into cDNA was done using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Genomics 2023Quote: ... DNA-Seq libraries were prepared from 1 mg of DNA extracted from young leaves using the Kapa LTP library prep kit (Kapa Biosystems, MA, USA). After quantity and quality evaluation with the High Sensitivity chip of a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1% Triton X-100, 1 mM DTT, 1 mM PMSF, 1× Roche protease inhibitor cocktail) and about 500 μL of 0.5-mm-diameter glass beads ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... at pH 7.4] and seeded at a density of 50–75 × 103 cells/plate on laminin (Roche) coated plates ...
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bioRxiv - Microbiology 2020Quote: Square plates containing standard Middelbrook 7H10 OADC agar were prepared and supplemented with kanamycin (Roche,20ug/ml), with or without anhydrotetracycline (ATc ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time PCR reactions were performed in 96-well plates on a Light Cycler II 480 (Roche) device ...
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bioRxiv - Plant Biology 2019Quote: ... Each cDNA sample was amplified in duplicate on a single 96-well optical plate using LightCycler480 (Roche). The cycling profile consisted of 95°C for 10 min followed by 40 cycles of 15 s at 95°C and 60 s at 60°C ...
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bioRxiv - Biochemistry 2021Quote: ... The plate was sealed and heated in a real-time PCR system (Light Cycler 480, Roche diagnostic) from 20°C to 80°C in increments of 0.5°C/minute ...
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bioRxiv - Plant Biology 2020Quote: Quantitative PCR (qPCR) was performed in triplicate using a 96-well plate-based (Roche Diagnostics, Penzberg, Germany) in a Thermal Cycling StepOnePlus (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... as per manufacturer’s instructions in 96-well plates and conducted in a LightCycler 480 Instrument II (Roche). Primers used in the study (Table S1 ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and developed with 50 μL of POD 3,3’,5,5’-tetramethylbenzidine (TMB) substrate (Roche) for 5 min and stopped with 50 μL of 1 M H2SO4 ...
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bioRxiv - Immunology 2022Quote: ... All the qPCR reactions were performed in a 96-well plate on Lightcycler 480II (Roche™, Germany) and data normalized to GAPDH and β-Actin ...
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bioRxiv - Plant Biology 2023Quote: ... in 384-wells plates with a total volume of 10 uL using Light Cycler 480 apparatus (Roche). mRNA abundance was compared to two reference genes EXP (AT4G26410 ...
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bioRxiv - Developmental Biology 2023Quote: ... QPCRs were performed using a 96-well plate qPCR machine (Stratagen) with SYBR green with ROX (Roche).