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Citations for Roche :
1 - 50 of 1950 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibody: rat-derived monoclonal anti-HA antibody (dilution 1:250; Roche). Secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μg of ant-HA antibody (Roche) was added and mixed at 4ºC for 1 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
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bioRxiv - Cell Biology 2020Quote: hESC H9 cell line was transfected with 2ug of pB-CAG-Dest-pA-pgk-bsd-CENP-A-SNAP plus 2ug of pBASE plasmid (harbouring the piggybac transposase, kind gift from José Silva) using FuGeneHD (Roche), in a ratio of DNA:FuGene of 1:3 ...
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bioRxiv - Neuroscience 2022Quote: NPCs derived from cynomolgus macaque (Cips_BC671A_NSC line generated at Roche) were differentiated in NPC differentiation medium (42) ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse lung digests are derived by 1mg/mL collagenase A (Roche) and DNAse I (50units/mL ...
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bioRxiv - Biochemistry 2021Quote: ... glycans were removed from lysate-derived gp120 or gp160 with Endoglycosidase H (Roche) treatment of the immunoprecipitates as described before (Land et al. ...
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bioRxiv - Genomics 2023Quote: ... For 3-color detection the following antibody dilutions were made: anti-digoxigenin (Roche, cat. 11333089001) 1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... per sample was washed 3 times with cold 1X PBS and 2μg anti-GFP monoclonal antibody (Roche) per sample was conjugated with Dynabeads in 1ml cold PBS at 4°C for 4h ...
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bioRxiv - Immunology 2022Quote: Stock solutions of collagen were prepared by dissolving 10 mg collagen I (rat tail-derived; Roche) in 3.3 mL of 0.2% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... PAC-derived probes were extracted from bacterial cultures and fluorescently labelled by nick translation (Roche, Mannheim, Germany). Following hybridisation and washes according to published protocols (Federico et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated with 20 mU ml- 1 Vibrio cholerae-derived neuraminidase (sialidase) (#11080725001, Roche, Switzerland) for 1 h at 37°C on a cell shaker ...
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bioRxiv - Neuroscience 2024Quote: IPSC-derived dopaminergic neurons were lysed in 1% SDS supplemented with protease inhibitors (Roche, cat no. 11697498001) and immediately boiled at 95°C for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... paraffin-embedded sections derived from Mtb-mouse mouse lungs on a Ventana Discovery Ultra autostainer (Roche Diagnostics). Following dewaxing and rehydration on board ...
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bioRxiv - Genomics 2021Quote: ... we blocked the slides for 1-hr using 3% BSA/PBS with 0.1% Tween and incubated slides with 1:200 secondary antibodies (Roche) in 3% BSA/4X SSC with 0.1% Tween and BSA at room temperature for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa Kyoto cells were transfected with linearized pcDNA3.1(+) derived plasmids using X-tremeGENE 9 DNA Transfection Reagent (ROCHE) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Immunology 2022Quote: ... the slides were washed in TBS/T 3 times and then incubated with secondary antibodies (2 µg/ml) and DAPI (1 µg/ml, Roche) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Hybridised probes were detected with anti-DIG antibodies and revealed with alkaline phosphatase-conjugated antibodies in presence of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Neuroscience 2023Quote: ... Washes were followed by blocking in 10% heat-inactivated sheep serum for 1-3 hours and incubation in buffer containing sheep antidigoxigenin antibody (Roche) at 1:5000 dilution for 16-20 hours at 4oC ...
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bioRxiv - Plant Biology 2022Quote: ... Expression data derived from Cp values calculated according to the second derivative maximum method (LightCycler®LC480 II, Roche) and normalized to the expression of PDF2 (At4g04890) ...
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bioRxiv - Biophysics 2021Quote: ... 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS, Roche Life Sciences, Indianapolis, IN) micelles and also using 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC ...
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bioRxiv - Biophysics 2022Quote: ... B1 and ACS elements) was amplified from the yeast chromosome (S288C_ChrIV BK0069382: 462,279–462,787) and inserted into λ DNA (Roche, Cat# 11558706910) with XhoI and NheI restriction enzymes (New England BioLabs) ...
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bioRxiv - Genetics 2022Quote: DNA sequences corresponding to the different donor/daughter mouse L1 elements were amplified from genomic DNA using Expand Long Range dNTPack (Roche). Reaction mixes contained 5 μl 5x Expand Long Range Buffer with 12.5 mM MgCl2 ...
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bioRxiv - Genetics 2020Quote: A comprehensive list of coordinates of all the exonic and conserved regulatory elements from human X chromosome was used to design a customized capture library from Roche, NimbleGen (Supplementary Table 1) ...
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bioRxiv - Immunology 2023Quote: ... 3 mM ATP (Roche), 25 μg/ml MSU (InvivoGen) ...
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bioRxiv - Microbiology 2022Quote: A labeled DNA probe for the new virus derived from Mr was prepared using a commercial PCR DIG-labeling mix (Roche)according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Neuroscience 2024Quote: ... specific digoxigenin (DIG)-labeled RNA probes and performed nitro blue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate color-reaction (BCIP) after wash and incubation with anti-digoxigenin antibody (catalog. no. 11093274910; Roche; RRID: AB_514497) as previously reported (Zempo et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’/3’RACE Kit (Roche) was used to amplify the RNA obtained and the final DNA was sequenced to obtain the +1 nucleotide of str ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Immunology 2024Quote: ... antibody-derived oligomers were amplified and indexed by mixing 45 μL eluent with 50 μL of KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and 5 μL of forward/reverse indexing primers (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 3% BSA in HBSS buffer for 20 min and incubated with a primary antibody (rat anti-HA, Roche, RD11867423001, 1:100) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)) supplemented with 1X protease inhibitor cocktail (Complete; Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 mg/ml Dispase II (Roche), and 1 mg/ml trypsin inhibitor (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and ∼3 mg DNase I (Roche). MhOR5 was extracted using 0.5% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% NP40 (Roche 11332473001), 3 µL 10% Tween-20 (Roche 11332465001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and dithiothreitol (Roche, 3483-12-3). Lysates were rocked at 4C for 20 min and centrifuged 10 min at 15,000g ...
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bioRxiv - Immunology 2023Quote: ... 3 IU/mL erythropoietin (EPO; Roche), 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Neuroscience 2024Quote: ... 3% SDC and PhosSTOP™ (Roche) by sonication at 40% amplitude for 4x10 sec ...
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bioRxiv - Immunology 2024Quote: ... THP-1-monocyte-derived IFN-γ-primed macrophages were induced by co-stimulation with PMA and 100 U/ml human IFN-γ (Roche, 11040596001) for 24 h ...
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bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
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bioRxiv - Biochemistry 2021Quote: ... 5mM CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) and 1 tablet of Protease Inhibitor Cocktail (ROCHE, cOmplete™). The preparation was sonicated for 120 s on ice ...
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bioRxiv - Microbiology 2020Quote: ... Universal probe library (UPL) probe #3 (Roche) and following primers were used for analysis of wtAAV2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% Tween-20 (Roche 11332465001), freshly add 3 µL 1:1 water diluted digitonin (Promega G9441) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg/mL Dispase II (Roche, 04942078001), and 0.1 mg/mL DNase I (Sigma ...