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Citations for Roche :
551 - 600 of 6051 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: DNA was extracted from mouse ear biopsies using High Pure PCR Template Preparation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
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bioRxiv - Genetics 2022Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) and cOmplete protease inhibitors (Roche) was added to prevent protein degradation ...
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bioRxiv - Neuroscience 2021Quote: DNA was extracted from tail or ear clippings using the Kapa Mouse Genotyping Standard kit (KAPA Biosystems) and stored at -20°C ...
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used in this study were as follows: mouse anti-HA antibody (Roche Life Science), mouse anti-PGK1 monoclonal antibody (Thermo Fisher/Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... mouse tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1 mL of DMEM medium supplemented with 2% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoblotted with a mouse monoclonal anti-HA antibody (clone 12CA5; Roche, 11 583 816 001, RRID:AB_514505). Rabbit monoclonal anti-PSTAIR (anti-Cdc2 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), mouse anti-Pgk1 (459250 ...
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bioRxiv - Cancer Biology 2023Quote: ... bound antibodies were detected with an anti-mouse HQ detection system (catalog# 7017936001 with 7017782001, Roche Diagnostics) and a Chromomap DAB IHC detection kit (catalog # 5266645001 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1, Roche) and protein G sepharose beads or ALFA selector ST beads (NanoTag Biotechnologies) ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used in this study were mouse monoclonal antibodies: GFP (clones 7.1 and 13.1; 11814460001; Roche, Germany) (1:2,000 for WB) ...
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bioRxiv - Cell Biology 2021Quote: ... 1% BSA (Roche), 20% Dextran Sulfate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 1 × PhosSTOP (Roche)] and incubated on ice for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... 1× PhosSTOP (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× cOmplete (Roche), and lysed by bead-beating ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP40 (Roche) and a phosphatase inhibitor mixture containing 20 mM NaF ...
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bioRxiv - Biochemistry 2022Quote: ... 1 × PhosSTOP (Roche)] and incubated on ice for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... 1/1000 (Roche); HRP-conjugated anti-FLAG antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... PhosSTOP 1% (Roche)) and mechanically with a pellet pestle ...
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bioRxiv - Cell Biology 2021Quote: ... mitotic cells were obtained by mitotic shake-off and swelled in hypotonic buffer (75 mM KCl:0.8% NaCitrate:H2O at 1:1:1) with protease inhibitor cocktail (Roche) at room temperature for 10-15 min ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were generated by lysing cells in RIPA buffer (50 mM Tris-Cl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1× Roche complete mini-protease inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... pH 6.8, 10 mM DTT, 1 mM EDTA, 0.1% Tween, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche). The samples were centrifuged at 3000g for 6 min at 4°C using a tabletop centrifuge ...
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bioRxiv - Genomics 2024Quote: ... 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, 1× Protease Inhibitor cocktail, Roche) and incubated at 4 °C for 1 hour with rotation ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL trypsin solution (100 ng μL−1 Sequencing grade, Roche Deutschland Holding GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM phenylmethanesulfonyl fluoride (PMSF) and 1 X protease inhibitors (Roche)] ...
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bioRxiv - Cell Biology 2020Quote: ... 1% sodium deoxycholate and 1 × EDTA-free protease inhibitor cocktail (Roche) supplemented with Cycloheximide (100 μg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-HA (1:10,000; Pierce) or Anti-GFP (1:2000; Roche) served as primary Abs ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg/ml aprotinin/pepstatin/leupeptin and 1 mM PefaBloc (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 1 x cOmplete protease inhibitor cocktail (Roche) with three rounds of freezing in liquid nitrogen and rapid thawing ...
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bioRxiv - Neuroscience 2020Quote: ... anti-EM48 (1:500; Chemicon) or anti-HA (1:500; Roche) antibodies and developed with biotinylated secondary anti-rabbit (1:500 ...
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bioRxiv - Physiology 2020Quote: ... digested for 1 hour with collagenase (Roche, 11088831001, 1 mg/ml) in RPMI medium at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 mM DTT and 1 × EDTA-free protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation at 17,000 ×g ...
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bioRxiv - Plant Biology 2021Quote: ... 10% glycerol) containing 1 mM PMSF and 1× protease inhibitor (ROCHE) followed by incubation for 30 min at 4°C (shaking) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sarkosyl and protease inhibitor (1 tablet per 10 ml, Roche), and incubated for 60 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 1% Triton X-100,10% glycerol) containing 1× Protease Inhibitor Cocktail (Roche) and incubated for 30 min at 4℃ ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM PMSF and 1 x complete protease inhibitor cocktail (Roche). Immunoprecipitation experiments with GFP-trap beads were performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed by 1 mg mL-1 Collagenase-D (Roche, Basel, Switzerland) for 10 min ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM DTT) supplemented with 1 complete protease inhibitor tablet (Roche). 40 mg/mL lysozyme was added to the pellets ...
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bioRxiv - Molecular Biology 2019Quote: ... 14 ml lysis buffer (100 μg/ml Lysozyme, 1 % Triton, 1 mM PMSF, 1 x protease inhibitor (cOmplete, Roche), 10 mM DT ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000; Roche 12CA5 HA at 1:2,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... were lysed in IP buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF and Roche complete EDTA free protease inhibitor cocktail) ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 mM KCl, 0.05 mM EDTA, 10% Glycerol, 1 mM DTT, 1 mM PMSF, 1× Complete Mini protease inhibitor, Roche) resuspended in RIPA buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Cell Biology 2022Quote: ... The nuclei pellet was lysed in 400 μl RIPA buffer 1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) and incubated on ice for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 mM protease inhibitor cocktail (1 mM EDTA, 20 mM NaF, 0.5% NP-40, 1% Triton X-100) (Roche) and 0.5% Na3VO4 on ice ...
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bioRxiv - Microbiology 2021Quote: ... and LiCl (250 mM LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 20 mM Tris-HCl PH8.0, complete protease inhibitor [Roche]) washing buffers ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). 20µl of HPC4 beads were supplied with 1mM Ca2+ ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). Clarified extracts were incubated for 2 h with agarose beads previously coupled to 10 µg of non-immune rabbit IgG or 10 µg of affinity purified Arpin antibodies (according to the manufacturer protocol ...