Labshake search
Citations for Roche :
151 - 200 of 5933 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... then subject to second-strand synthesis using 1.5 μl biotinylated oligo (10 μM) and 25 μl KAPA Hi-Fi hot start ready mix 2x (KK2601, Roche). Second strand reactions were incubated at 95°C for 3 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
-
bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
-
bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
-
bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
-
bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
-
bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
-
bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
-
bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
-
bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
-
bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.01 mg/mL human insulin (Roche, Indianapolis, IN, USA), 100 IU penicillin (Mediatech) ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... and human recombinant IL-2 (30 U/ml, Roche) for 72 h.
-
bioRxiv - Cancer Biology 2023Quote: ... human PR rabbit monoclonal antibody (Roche Diagnostics, 790-4296), and human Ki67 rabbit monoclonal antibody (Roche Diagnostics ...
-
bioRxiv - Immunology 2023Quote: ... and 10 ng/ml recombinant human IL-2 (Roche). For activation of murine CD4+ T cells ...
-
bioRxiv - Immunology 2023Quote: ... human interleukin-2 (IL-2) (10 IU/ml, Roche), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
-
Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
-
bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
-
bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
-
bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
-
bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
-
bioRxiv - Cell Biology 2021Quote: ... human NEMO (5 µg) and ATP (2 mM) (Roche, 10519979000) were incubated at 37°C for the indicated time in a buffer containing 150 mM NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... coated with 5 µg/ml human plasma fibronectin (Roche, 11080938001) in growth medium overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
-
bioRxiv - Immunology 2023Quote: ... 100 IU/ml of recombinant human IL-2 (Roche Diagnostics) or 10 μg/ml of SIVmac251 Env gp130 (ImmunoDx) ...
-
bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.01 mg/mL of human insulin (Roche, Indianapolis, IN, USA), 10 mM HEPES (Corning ...
-
bioRxiv - Cancer Biology 2021Quote: ... + 0,4 g/l of human albumin (Vialebex) with Liberase TL (Roche) 150 ug/ml and DNase 1 (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
-
bioRxiv - Genomics 2019Quote: ... using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture ...
-
bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
-
bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
-
bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...