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Citations for Roche :
201 - 250 of 7020 citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... libraries are quantified using quantitative PCR (kit purchased from KAPA Biosystems) with probes specific to the ends of the adapters ...
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bioRxiv - Genomics 2019Quote: K111 insertions were amplified by PCR using the Expand Long Range dNTPack PCR kit (Roche Applied Science, Indianapolis, IN) as described ...
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bioRxiv - Neuroscience 2021Quote: ... The eaat2a target site was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) (primers ...
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Activation of the Integrated Stress Response overcomes multidrug resistance in FBXW7-deficient cellsbioRxiv - Cancer Biology 2022Quote: ... The U6-sgRNA cassette was then amplified by PCR using the KAPA HIFI Hot Start PCR kit (Roche, KK2502) and different tagged primers required for the subsequent Illumina sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Genomics 2023Quote: ... Adapter-ligated material was amplified with 12-14 cycles of PCR using KAPA HiFi HotStart PCR Kit (Roche, 07958897001) and universal P5/P7 Illumina primers (see Golov et al. ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Genotyping was carried out on DNA extracted from ear tissue following manufacturer’s protocols (Express Extract, Kapa Biosystems, Cambridge, MA, USA). TaKaRa Ex Taq polymerase (RR001 ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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bioRxiv - Developmental Biology 2021Quote: Mice ear notches or embryo tail tips were genotyped by PCR using Kapa2G Robust HotStart PCR Kit (Kapa Biosystems, KK5517) and Lbx1 primers (Table S1) ...
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bioRxiv - Developmental Biology 2021Quote: ... The levels of relevant mRNAs were quantitated by real-time PCR using One Step SYBR GREEN RT-PCR Kit (Roche) in a Light Cycler instrument (Roche Applied Science ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplicons were purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics Gmb, Mannheim, Germany). Sequencing was performed using MiSeq™ Illumina® (2 x 300 bp ...
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bioRxiv - Bioengineering 2019Quote: ... the entire miniprepped volume was used as template in a PCR reaction using the KAPA HiFi PCR Kit (Kapa Biosystems). A ten-fold dilution of this PCR product was used as template in a barcoding PCR adding Illumina adapter sequences and dual barcodes ...
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bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems, Boston USA) and 12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of DNA was subjected to PCR amplification using the Kapa HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, USA). The individual PCR reactions were performed in 20 μL final volume and contained:
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the LightCycler 480 SYBR Green I Master kit (Cat. 04887352001, Roche). Primers for pmp-3 and ACTIN were used as housekeeping for C ...
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bioRxiv - Microbiology 2021Quote: Alkali-labile digoxigenin labelled deoxyuridine triphosphate (DIG-dUTP) was incorporated in Southern blot probes by PCR using PCR DIG Probe Synthesis kit (Roche) with the following specifications (refer to sequences in Supplementary table 3) ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Plant Biology 2023Quote: ... We used the SYBR Premix Ex Taq II reagent kit (Vazyme, Q311-02) to perform qRT-PCR on a LightCycler 96 Real-Time PCR System (Roche). Rice OsACTIN1 was used as the internal reference ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out using the KAPA HiFi Hot Start Ready Mix PCR Kit (Cat# KK2602, KAPA Biosystems, Wilmington, MA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Quantitative real-time PCR (qRT-PCR) analysis was performed using the KAPA SYBR FAST qPCR kit (KAPA Biosystems Inc., Switzerland) to check the gene expression levels of GLXI;1 ...
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bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Immunology 2024Quote: ... One hundred ng of genomic DNA was used for PCR amplification of the target loci using KAPA HiFi HotStart PCR kit (Roche) and the primer sets in Table S1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... which was then amplified using the KAPA HiFi PCR Kits (Roche, USA). The amplified products were classified into different fragments according to their sizes (1~2 k ...
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bioRxiv - Molecular Biology 2020Quote: ... DIG-labelled probe prepared using PCR DIG Probe Synthesis Kit (#11636090910, Roche) was added and the membranes incubated for over 18 h ...
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bioRxiv - Bioengineering 2020Quote: ... using Expand High-Fidelity PCR Kit according to the manufacturer’s instructions (Roche). The PCR reaction consisted of 5 μl of 10x reaction buffer ...
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bioRxiv - Cell Biology 2021Quote: ... with KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems, MI). NCX ...
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bioRxiv - Cell Biology 2021Quote: ... gDNA was extracted using the High Pure PCR Template Preparation Kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Quantitative RT-PCR was performed using the SYBR Green kit (Roche, Belgium) with 100 nM primers and 0.125 μl of RT reaction product in a total volume of 5 μl per reaction ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified using the High Pure PCR Product Purification Kit (Roche) and afterwards restricted with BamHI (ThermoScientific ...
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bioRxiv - Microbiology 2021Quote: ... using the KAPA SYBR FAST One-Step qRT-PCR kit (Kapa Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... which were generated by a DNA-labelling PCR-Kit (Roche Life Science).
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bioRxiv - Microbiology 2021Quote: ... and purified by High Pure PCR Product Purification Kit (Roche, Mannheim, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... bead washes and PCR amplification using the HyperCapture Target Enrichment Kit (Roche). Samples were sequenced by paired-end sequencing with a 300 cycle high-output Nextseq 500 kit (Illumina) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was subject to PCR using a Taq DNA Polymerase kit (Roche) using primers specific for mouse Ocm and Glyceraldehyde 3-Phosphodehydrogenase (G3PDH) ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified using the High Pure PCR Product Purification Kit (Roche) and afterwards restricted with EcoRI (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... All sequencing used PCR-free library preparation kits purchased from KAPA Biosystems, equivalent to the protocol in the Illumina TruSeq PCR-Free Sample Preparation Guide (Illumina cat# FC-121-2001) ...
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bioRxiv - Molecular Biology 2019Quote: ... de-crosslinked and purified using High Pure PCR Cleanup Micro Kit (Roche). The libraries were prepared using TruSeq ChIP Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA was purified using High Pure PCR Cleanup Micro Kit (Roche). DNA libraries were prepared using TruSeq ChIP Sample Preparation Kit (Illumina ...
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bioRxiv - Systems Biology 2022Quote: ... library preparation was performed using a KAPA HyperPrep Kit PCR-free (Roche) with a target insert size of 500 bp ...
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bioRxiv - Cell Biology 2022Quote: ... and amplified for 15 cycles using KAPA HiFi PCR Kit (KAPA biosystems). cDNA was then separated into two fractions by size using 0.5X and 1X AMPure PB Beads (Pacific Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR was performed using Kapa SYBR FAST qPCR kit (Kapa Biosystems).
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bioRxiv - Genomics 2024Quote: ... All PCRs were performed using the KAPA HiFi Kit (Roche, Basel, Switzerland).
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bioRxiv - Genetics 2021Quote: ... UK) following the manufacturer’s instructions and endpoint genotyping was conducted from fluorescence using a Lightcycler 480 Instrument II (Roche, Indianpolis, IN). The previously reported ASR gene Yr10 (Frick et al. ...
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bioRxiv - Genetics 2021Quote: ... UK) following the manufacturer’s instructions and endpoint genotyping was conducted from fluorescence using a Lightcycler 480 Instrument II (Roche, Indianpolis, IN). The Rht markers were coded as lines with Rht-B1b (1) ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was then used to perform qRT PCR using the KAPA SYBR FAST qRT PCR master mix kit (KK4602, KAPA Biosystems) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems, Wilmington, MA, USA) followed by size selection from 1.5kb to 3.5kb with a BluePippin system (Sage Science ...