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Citations for Roche :
251 - 300 of 4984 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... and library preparation and purification performed using KAPA HiFi Hot Start DNA Polymerase (KAPA Biosystems, KK2500) and Ampure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2022Quote: ... 5 mM imidazole and 500 μL cOmplete Hig-tag purification Resin (obtained from Roche, ref 05893682001). The mixture was gently shaken at 4°C for 4 h in dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Biochemistry 2023Quote: ... protein eluates from every purification step were immediately supplemented with protease inhibitors (EDTA-free cOmplete, Roche) (together with DTT at 10 mM for cysteine-containing protein constructs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The purification protocol was identical to other samples with a couple of exceptions: Protease inhibitor mix (ROCHE) and 0.5 mM TCEP were included in the sample before lysis ...
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bioRxiv - Microbiology 2021Quote: ... Isolation and purification of viral RNA was performed using the MagNA Pure 96 System (Roche, Penzberg, Germany) according to the manufacturers’ recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.002% Tween-20 for Ni-NTA purification) and EDTA free protease inhibitor cocktail (Roche, Basel, Switzerland). The suspended cells were disrupted by sonication and then cell debris were separated by centrifugation at 48,384g for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) supplemented with one tablet ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche). The cell solution on ice was sonicated 3 × 1 min at 90% amplitude (0.5 s cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... and supplemented with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablet (Roche). Cells were lysed via high-pressure homogenization ...
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bioRxiv - Genomics 2019Quote: ... 5.6mmol/L glucose with 2% BSA fraction V fatty acid free (Roche Diagnostics), 50μmol/L 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant removed and ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche, Lewes, UK) added to cell-free media ...
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bioRxiv - Pathology 2023Quote: ... 0.1% SDS and 0.5% deoxycholic acid) with cocktail protease and phosphatase inhibitors (Roche) at 4℃ condition ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) supplemented with an EDTA-free antiprotease tablet (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... Basal respiration was measured in DMEM containing 2% fatty-acid-free BSA (Roche). Cells were treated with 100 μM isoproterenol to stimulate respiration ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2% bovine serum albumin (BSA; fraction V, fatty-acid-free; Roche), 50 µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants containing the His-tagged histones were combined with NiNTA resin (Complete His-Tag purification Resin, Roche) (1mL of Ni-NTA per 1L of bacteria ...
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bioRxiv - Biochemistry 2022Quote: ... the AAC2 variants were imported into Tom40-HA mitochondria followed by affinity purification via anti-HA beads (Roche) (18) ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Genetics 2020Quote: ... 0.25% deoxycholic acid) plus protease inhibitor and phosphatase inhibitor Data Setts (Roche Applied Science). 11 μg protein was separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with phenylmethanesulfonyl fluoride solution and a protease inhibitor cocktail with ethylenediaminetetraacetic acid (Roche). Brain tissue was homogenized in ice-cold RIPA buffer (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasma concentrations of free fatty acid and glycerol were analyzed with reagents from Roche and Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM ethylenediaminetetraacetic acid) supplemented with protease and phosphatase inhibitors (Sigma and Roche). Proteins were immobilized on PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... and ADNaCl (0.5% fatty acid free BSA from Roche, 0.2% dextrose and 0.85% NaCl) or in Middlebrook 7H10 supplemented with 0.5% glycerol and 10% oleic acid-albumin-dextrose-catalase (OADC) ...
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bioRxiv - Biophysics 2023Quote: ... The lysate supernatant was mixed with 200 µL of Ni-nitrilotriacetic acid resin (Roche Complete His-Tag purification resin ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM ethylenediaminetetraacetic acid (EDTA)) supplemented with complete EDTA-free protease inhibitor cocktail (Roche), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-Fab were expressed with His-tag and purified using the cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... [U-13C]palmitate was first non-covalently conjugated to ultra fatty acid free BSA (Roche) as previously described (Vacanti et al. ...
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bioRxiv - Pathology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% protease inhibitor cocktail set III ethylenediaminetetraacetic acid(EDTA)-free (Roche, Shanghai, China). Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Lineage Cell Therapeutics ...
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bioRxiv - Microbiology 2022Quote: Prior to quantitative amino acid analysis Cel1cat was de-glycosylated using EndoH (Roche Diagnostics GmbH). The de-glycosylation was carried out in 20 mM MES ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). The protein concentration was measured using BCA Protein Assay Kit (Thermo Fisher Scientific) ...