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Citations for Roche :
251 - 300 of 7538 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... at 95°C for 5 min (90°C for 15 sec, 60°C for 60 sec) × 50 cycles (Light/Cycler Nano, Roche). The primer sets used in this study were described in the supplemental Table 1.
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bioRxiv - Microbiology 2022Quote: ... 95°C), annealing (10 sec, 58°C) and elongation (10 sec, 72°C) performed on a LyghtCycler 96 Instrument (Roche). MIC14 and MIC15 transcripts were amplified with the primer pairs P64/P65 and P66/P67 ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was measured using the Cell Proliferation Kit I (Roche, Basel, Switzerland) in a 3-fold determination ...
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bioRxiv - Developmental Biology 2021Quote: ... To detect cell death the “In Situ Cell Death AP kit” (Roche, #11684809910) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using the Cell Proliferation Kit I (MTT) (Roche, #11465007001) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were carried out with a Roche LightCycler 480 system (384-well format, Roche Applied Science, Indianapolis, IN, USA) using a RT/HRM (real-time PCR/high-resolution melt ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by 20 cycles of polymerase chain reaction (PCR) amplification using KAPA HiFi HotStart ReadyMix (#KK2602, Roche, Basel, Switzerland) and subsequent purification with Agencourt AMPure XP beads as described ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Pre-amplification of the V3V4 region of 16S rRNA was performed by PCR in triplicate using 2xKAPA HiFi Hot Start ReadyMix (Roche) using primer pairs containing adaptor sequences ...
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bioRxiv - Microbiology 2021Quote: ... 5 ul of this solution was used for amplification of the SLST region linked to illumina adaptors using KAPA HiFi HotStart Readymix (Roche) (Initial denaturation for 5 min at 95 °C followed by 35 cycles of 98 °C for 20 s ...
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bioRxiv - Microbiology 2020Quote: ... the 3.5 Kb regions flanking either side of tdk were amplified with Kapa HiFi Taq MasterMix (Kapa Biosystems, Wilmington, MA) and joined by splicing overlap exchange (SOE ...
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bioRxiv - Cell Biology 2022Quote: Sense and anti-sense DIG-labelled RNA probes were synthesised from 5’ and 3’ regions of tert using DIG RNA Labelling Mix (Roche). A 562bp 3’ region of tert ...
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bioRxiv - Cancer Biology 2019Quote: ... was designed to tile a 250 bp capture region within the central 4-kb target region from each 10-kb window of hg19 avoiding non-specific sequences by Roche so that the maximum distance between 2 capture regions is 14-kb ...
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bioRxiv - Epidemiology 2019Quote: ... The first round of PCR amplified the 73-V4 region using KAPA HIFI HotStart Ready Mix (Kapa Biosystems, Wilmington, MA) with following PCR conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... The target regions were amplified for each sample using the primer pools coupled with KAPA HiFi Hotstart readymix (KAPA Biosystems) using 20 µM primers as well as a 50/53 °C (Annealing temperature 1/2 ...
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bioRxiv - Biophysics 2021Quote: ... Anti-c-Myc (Roche) molecules were covalently linked to the carboxylated polystyrene beads (Spherotech ...
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bioRxiv - Molecular Biology 2021Quote: ... or Glu-C (Roche) at 25 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Mitomycin C (Merck (Roche), 10107409001) ...
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bioRxiv - Microbiology 2024Quote: ... mitomycin C (Roche Diagnostics) as prophage-inducing reagent [73] was added in triplicates in different final concentrations ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription products were then amplified by quantitative PCR (95 °C, 5 minutes; 95 °C, 15 seconds; 60 °C, 60 seconds; 40 cycles) (LightCycler® 480, Roche) using TaqMan Universal PCR Master Mix and specific probes for miRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... cells were isolated from mouse brains with Collagenase D (0,5 mg/ml, #11088866001, Roche), DNase I (10μg/ml ...
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bioRxiv - Genetics 2019Quote: ... Cells were lysed and cleared extracts incubated with Mouse anti-MYC (9E10) antibodies (Roche) to immunoprecipitate MYC epitope tagged proteins as previously described (Bloom et al. ...
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bioRxiv - Cell Biology 2023Quote: Mouse wound beds were dissected and digested into single cells using Liberase TM (Roche) and cells were suspended in fluorescence-activated single cell sorting (FACS ...
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bioRxiv - Molecular Biology 2021Quote: ... HCXII-0155) was incubated with neutrophils that were pre-treated with vehicle or DNase I (10U/ml, Dornase alpha, Roche, Germany) for 1h ...
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bioRxiv - Immunology 2024Quote: ... the cells were quickly transferred into 10 ml of pre-warmed RPMI supplemented with 10% FBS and 100µg of Pulmozyme (dornase alpha, Roche, Basel, Switzerland) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryonic tail biopsies were used for rapid genotyping using KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352). All mouse protocols and experiments were approved by the Rutgers Institutional Animal Care and Use Committee ...
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bioRxiv - Pathology 2021Quote: DNA extraction and PCR were performed using Kapa mouse genotyping kit (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Cell Biology 2019Quote: ... The apoptotic cells were defined by using In Situ Cell Death Detection Kit (Roche) following manufacturer’s protocol and quantified by calculating positively stained cells in at least five randomly chosen HPFs of each slide.
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bioRxiv - Microbiology 2021Quote: ... Apoptotic cells were quantified by the In situ cell death detection kit (12156792910, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Relative cell viability was assessed using the XTT cell proliferation Kit II (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Apoptotic cells were visualized using the In Situ Cell Death Detection Kit (Sigma/Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... For TUNEL staining (In Situ Cell Death Kit, Roche), fresh frozen transverse cryostat sections (30 μm ...
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bioRxiv - Neuroscience 2022Quote: ... In Situ Cell Death Detection kit (Roche Cat# 11684795910) was used to assess cell death.
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bioRxiv - Zoology 2019Quote: ... Polymerase Chain Reaction (PCR) amplification was conducted in 12.5 μl reaction volumes consisting of AmpliTaq® DNA polymerase (Roche Molecular Systems, Inc) forward and reverse primers (0.5 μM each) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) analysis of cDNA expressions was conducted using SYBR FAST Universal qPCR Master Mix (Kapa Biosystems; #KK4602) with a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Abutting primer pairs were designed that were specific to these genetic groups (Supplementary Table 3) to enable the recovery of full viral genomes using polymerase chain reaction (PCR) with HiFi HotStart DNA polymerase (Kapa Biosystems, USA) using cycling conditions per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT– PCR) was performed in optical 96-well plates via the LightCycler 480 II system (Roche Life Science) using KOD SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were quantified by Reverse Transcription quantitative polymerase chain reaction (RT–qPCR) using the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland). Cycling parameters were set at (i ...
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bioRxiv - Immunology 2024Quote: 293T cells were co-transfected with plasmids encoding cognate heavy and light chains using the Xtreme Gene 9 DNA transfection reagent (Roche, Basel, Switzerland), then grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
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bioRxiv - Microbiology 2019Quote: ... Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Purified DNA from mouse fecal pellets or sorted cells was subjected to 16S variable region 4 PCR amplification using barcoded 515F and 806R primers (47) and the KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), with the following cycling conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Biochemistry 2019Quote: ... Blocking reagent for ELISA (BRE) and cOmplete protease inhibitor tablets were from Roche (Basel, Switzerland). Phosphate buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: ... 100uL of the clarified supernatant was used in the anti-BrdU DNA fragmentation ELISA (Roche). Each condition was tested in technical triplicates ...
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bioRxiv - Neuroscience 2021Quote: ... NeuroMab; HCN2: mouse, 75-111, NeuroMab; HCN3: mouse, 75-175, NeuroMab; HCN4: mouse, 75-150, NeuroMab; HA: mouse, 12CA5, Roche) in 1% milk powder and incubated overnight at 4°C ...