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Citations for Roche :
351 - 400 of 5487 citations for Mouse Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Genomic DNA was extracted from 3 week old tail or ear biopsies using KAPA Mouse DNA Extraction Kit (KAPA Biosystems) or High Pure PCR Template Kit (Roche).
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bioRxiv - Cell Biology 2019Quote: All genotyping was done using the HotStart Mouse Genotyping Kit with its instructed PCR reaction set up from KAPA Biosystems. For the NF1Afl/fl genotype ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... were prepared by ligation of Illumina adapters on 100 ng of amplicons following the Kapa Hifi HotStart NGS library Amplification kit (Kapa Biosystems, Wilmington, MA, USA). After quantification and quality control ...
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bioRxiv - Bioengineering 2020Quote: ... After each sample was quantified with the home-brew ddPCR library quantification assay and KAPA SYBR FAST Universal qPCR Library Quantification Kit (#KK4824, Kapa Biosystems, Wilmington, MA, USA), all samples were pooled in equimolar quantities ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... and indexed libraries were created for sequencing using Truseq adapters and quantified using the Kapa universal qPCR library quantification kit (Kapa Biosystems Inc., MA, USA). Samples were sequenced on an Illumina Novaseq using a 2×100 paired-end chemistry kit (Illumina Inc. ...
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bioRxiv - Physiology 2019Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... The quality and quantity of the final libraries were determined using the Agilent 2100 Bioanalyzer DNA HS kit and Kapa Biosystems qPCR (Kapa Biosystems, Inc., Wilmington, MA). Multiplexed libraries were pooled and normalized to 17.5 pM ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA-Seq Library was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kits with 201-300 bp insert sizes (KAPA Biosystems, Wilmington, MA, USA) using 250 ng total RNA as an input ...
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bioRxiv - Molecular Biology 2020Quote: Equal amount by mole of non-targeted and targeted RNA-Seq library were mixed based on qPCR-based quantification with Kapa Library Quantification Kit (KAPA BIOSYSTEMS, Wilmington, MA, USA). Sequencing of 150-bp paired-ends using HiSeq was carried out.
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bioRxiv - Genomics 2021Quote: ... Quantification of final library before loading on MiSeq sequencer was performed using the Kapa quantification kit (RT-PCR) for next generation sequencing with the Illumina platform (Kapa Biosystems, Wilmington, MA USA). Sequence reads were quality filtered and assembled using CLC Genomics Workbench 8.0.1 (CLCbio ...
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bioRxiv - Genetics 2020Quote: ... Tag sequences for identifying individual trees were attached to the DNA fragments using the Access Array Barcode Library for Illumina Sequencers-384, Single Direction (Fluidigm Corporation, South San Francisco, CA, USA) and KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, Wilmington, MA, USA). PCR was performed in a 20 μL volume containing 2 μL of 20-times diluted PCR products ...
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bioRxiv - Neuroscience 2022Quote: ... tissue samples were taken by collecting ∼2 mm of the end of the tail and extracting DNA from the tissue sample using the Kapa Express Extract Kit (Kapa Biosystems, Wilmington, MA, USA). The extracted DNA was then subjected to PCR using in-house designed primers to identify WT and KO alleles ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... n=40) and brain (n=40) for each individual using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adapters (KAPA Biosystems; Wilmington, MA, USA), with 100 ng of large RNA as input ...
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bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Neuroscience 2023Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... and purified based on the manufacturer’s instructions before sample barcoding with xGen Stubby Adapter and Unique Dual Index Primers (IDT) and library preparation using KAPA HyperPrep Kit (Kapa Biosystems-Roche, Wilmington, MA). The library was then sequenced by Illumina MiSeq nano PE150 at the Georgia Genomics and Bioinformatics Core ...
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bioRxiv - Cell Biology 2023Quote: ... We used identical input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were analyzed using Agilent DNA 1000 chip to estimate the quantity and check size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835; Kapa Biosystems, Wilmington, MA, USA) prior to amplification with Illumina’s cBot ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-GFP (Roche) (1:1000).
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-GFP (Roche), mouse anti-Ack1 (A-11 ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-GFP (Roche), anti-VSV-G 41A1 and rabbit anti-GM130 (clone EP892Y ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-GFP (Roche) 1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... GFP (Mouse, Roche, 11814460001), GST (Mouse ...
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bioRxiv - Genetics 2022Quote: ... from mouse (Roche #11814460001) antibody and Anti-Mouse IgG −Alkaline Phosphatase antibody (A9316 ...
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bioRxiv - Plant Biology 2020Quote: ... mouse anti-GFP (Roche) 1:2500 ...
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Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... mouse anti-p16 (Roche, #805-4713 ...
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bioRxiv - Cell Biology 2022Quote: α-GFP (Roche, from mouse) 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-GFP (Mouse, Roche) (1/500) ...
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bioRxiv - Developmental Biology 2022Quote: ... The incorporation of BrdU was then measured by ELISA at 24h per the manufacturer’s protocol (Roche Diagnostics, Mannheim, Germany).
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bioRxiv - Systems Biology 2020Quote: ... We generated digoxigenin (DIG)-labeled mRNA antisense probes against Nrxn3 using reverse-transcribed mouse cDNA as a template and an RNA DIG-labeling kit from Roche (Sigma). Primer and probe sequences for the Nrxn3 probe is available in Allen Brain Atlas (http://www.brain-map.org) ...
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bioRxiv - Developmental Biology 2020Quote: PCR was performed on genomic DNA extracted from cell pellets or mouse tails to determine the loss of the Y chromosomal genes Uba1y and Ssty1 by utilizing KAPA Mouse Genotyping Kit (Roche, KK7302). Specific procedures were followed according to the reagent instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Vglut3 and fluorescein (FITC)-labeled mRNA against DBH using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Vglut1 ...
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bioRxiv - Developmental Biology 2024Quote: ... the tail of each embryo was collected for genomic DNA extraction using the KAPA Mouse Genotyping Kit HotStart (Kapa Biosystems, KK7352). Subsequently ...
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bioRxiv - Systems Biology 2020Quote: ... and quantitative PCR (Kapa Biosystems, Wilmington, MA, USA). 8 pools of 24 randomized libraries were sequenced in 3 lanes at 100 bp single-end on the HiSeq 2500 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... using Kapa DNA polymerase (Kapa Biosystems, Wilmington, MA) and screened with novel silent restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... using Kapa DNA polymerase (Kapa Biosystems, Wilmington, MA) and screened with novel silent restriction sites ...
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bioRxiv - Genomics 2021Quote: ... and quantified by qPCR (Kapa Biosystems, Inc., MA), pooled ...
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bioRxiv - Molecular Biology 2020Quote: ... 1U Kapa Taq (Kapa Biosystems, Wilmington, MA, USA) and 1μl of genomic DNA as template ...
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bioRxiv - Microbiology 2022Quote: ... and quantified (KAPA quantification, KAPA Biosystems, Wilmington, MA). An Illumina MiSeq (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Quality of libraries were determined via Agilent 2200 TapeStation using High Sensitivity D1000 tape and quantified using Kappa SYBR®Fast qPCR kit (KAPA Biosystems, Inc, Wilmington, MA). Approximately 60-80 million paired-end 150 np sequence reads were generated per sample using the Ilumina HiSeq 4000 sequencer.
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using the extracted DNA (100 ng) as template with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Library quality was assessed using a Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Kapa Biosystems, Inc, Wilmington, MA, USA). RNA-seq experiments have been performed on an Illumina HiSeq3000 using a paired-end read length of 2×150 bp with the Illumina HiSeq3000 sequencing kits.
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bioRxiv - Cell Biology 2019Quote: ... and levels of p24 were determined by ELISA as previously described (65) The SEAP levels of the supernatants were determined by a chemiluminescent method (Roche) and were used to normalize the differences in transfection efficiencies.