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Citations for Roche :
251 - 300 of 5999 citations for Mouse Mesoderm Specific Transcript Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Blocking reagent for ELISA (BRE) and cOmplete protease inhibitor tablets were from Roche (Basel, Switzerland). Phosphate buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: ... 100uL of the clarified supernatant was used in the anti-BrdU DNA fragmentation ELISA (Roche). Each condition was tested in technical triplicates ...
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bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... NeuroMab; HCN2: mouse, 75-111, NeuroMab; HCN3: mouse, 75-175, NeuroMab; HCN4: mouse, 75-150, NeuroMab; HA: mouse, 12CA5, Roche) in 1% milk powder and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: DNA was extracted from mouse ear biopsies using High Pure PCR Template Preparation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: DNA was extracted from tail or ear clippings using the Kapa Mouse Genotyping Standard kit (KAPA Biosystems) and stored at -20°C ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Immunology 2021Quote: ... Mouse (Roche) on the LightCycler 2.0 (Roche) ...
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bioRxiv - Plant Biology 2019Quote: The satellite-specific probes were labelled by PCR in the presence of biotin-16-dUTP (Roche). We used nick translation to mark the probes for the ribosomal genes ...
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bioRxiv - Neuroscience 2020Quote: ... we used gene-specific primers and Light Cycler® 480 SYBR Green I Master (Roche Diagnostics). Polymerase chain reaction conditions were as follows ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with Lpd-specific or scramble shRNA as control using Fugene HD (Roche) transfection reagent ...
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bioRxiv - Plant Biology 2019Quote: ... using gene-specific digoxigenin-nucleotide-labelled DNA probes (Digoxigenin-11-dUTP alkali-labile, Roche, Basel, Switzerland) obtained using the same primer pairs and conditions as for the PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time amplification was carried out using gene-specific primers and SYBR Green Master mix (Roche) on a Lightcycler LC480 apparatus (Roche ...
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bioRxiv - Synthetic Biology 2020Quote: ... specific mutations were introduced to the DNA polymerase gene by site-directed mutagenesis (Roche Sequencing Solutions) to enhance the kinetic properties of the polymerase utilizing polynucleotide tagged nucleotides to approach native nucleotide incorporation characteristics32 ...
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bioRxiv - Physiology 2020Quote: ... Eluted DNA was amplified with specific primers (Table S2) using SYBR Green Master (Roche, Basel, Switzerland), and the resulting signals were normalized to input activity.
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bioRxiv - Molecular Biology 2022Quote: ... One set specific to the GFP region includes the Universal probe library (UPL) probe #67 (Roche), the forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed using specific primers and the LightCycler 480 SYBR Green I (#04887352001, Roche) on a LightCycler 480 (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR was performed with specific primers (Table S2) and LightCycler 480 SYBR Green I Mastermix (Roche) using the LightCycler 480 II real-time PCR device (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Enrichment of the specific histone mark was quantified by qPCR using SYBR Green Master Mix (Roche) and qPCR primers 1.4 kb away from the DSB (3xp3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche).
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bioRxiv - Microbiology 2024Quote: ... interleukin-2 (IL-2; specific activity 10 U/ng) at concentration of 20 ng/mL (Roche) and phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Immunology 2022Quote: Full-length HIV clones were quantified by reverse transcriptase (RT) enzyme-linked immunosorbent assay (ELISA) (Roche) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... in presence of 250 nM of specific primers with Kappa SYBR Fast qPCR Master Mix (Kapa Biosystems) on a CFX96 touch real-time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... and qPCR analysis was carried out using specific primer pairs and SYBR green master mix (Kapa Biosystems) with a QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... and qRT-PCR was measured using gene-specific primers with FastStart SYBR Green PCR Master Mix (Roche) on a StepOne Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The individual library pools were amplified using subpool specific primers and KAPA HiFi HotStart ReadyMix (Roche, #KK2601). After PCR ...
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bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
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bioRxiv - Neuroscience 2021Quote: ... GFP (mouse, Roche, 11814460001 ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 μl gene specific primer mix (5 μM each) and 4.5 μl FastStart Essential cDNA Green Master (Roche) were amplified using 45 cycles of 25 s at 95 °C ...
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bioRxiv - Microbiology 2021Quote: ... The fragments of each segment were amplified with a linker sequence containing specific primers using Taq polymerase (Roche). Specific primers with a linker sequence were designed to amplify every 120 bp of the coding region with a 15-bp overlapped region (PCR primers were listed in Table S4) ...
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bioRxiv - Cancer Biology 2020Quote: ... The expression of KZFP was verified by western blotting with the HA-specific antibody (Roche, Basel, Switzerland; #12013819001). Empty vector-transduced A549 cells (referred to as negative control cells (NC cells) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µl gene specific primer mix (5 µl each) and 4.5 µl FastStart Essential cDNA Green Master (Roche) were amplified using 45 cycles of 25 s at 95 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
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bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
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bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
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bioRxiv - Developmental Biology 2021Quote: Genomic DNA was extracted from 3 week old tail or ear biopsies using KAPA Mouse DNA Extraction Kit (KAPA Biosystems) or High Pure PCR Template Kit (Roche).
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bioRxiv - Cell Biology 2019Quote: All genotyping was done using the HotStart Mouse Genotyping Kit with its instructed PCR reaction set up from KAPA Biosystems. For the NF1Afl/fl genotype ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...