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Citations for Roche :
251 - 300 of 9037 citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were pelleted by centrifugation (750 x g, 2 min, 4 °C) and resuspended in 50 μl vPBS containing EDTA-free protease inhibitor (Roche). The cells were fixed by addition of 0.5 ml ice-cold fixation solution (4% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies were used to detect green fluorescent protein (GFP) (Cat#11814460001, clones 7.1 and 13.1, Roche). Rabbit polyclonal anti-Cdc42p antibodies were provided by Dr ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Developmental Biology 2022Quote: ... containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche, 11175025910). A short bleaching was used to remove the pigments from the fixed zebrafish embryos.
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bioRxiv - Developmental Biology 2023Quote: ... All genotyping reactions were performed with KAPA HotStart Mouse Genotyping Kit (Roche, 07961316001) with 1 μL of gDNA and a final primer concentration of 5 μM each ...
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bioRxiv - Cancer Biology 2021Quote: ... containing protease (Roche) and phosphatase (Sigma ...
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bioRxiv - Physiology 2021Quote: ... containing PhosSTOP (Roche) and cOmplete ...
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bioRxiv - Cancer Biology 2021Quote: ... containing protease (Roche) and phosphatase inhibitors (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... containing protease (Roche) and phosphatase inhibitors (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... containing phosphatase (Roche) and protease (Roche ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2′3′-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in HM, resuspended in lysis buffer (SDS 2%, Tris-HCl pH 8.0 100 mM containing a mix of protease (Roche complete cocktail) and phosphatase inhibitors (Biotools cocktail or lab-made cocktail ...
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bioRxiv - Developmental Biology 2022Quote: ... pellets were dissolved with 300 µl 1X PMPG buffer (1x PBS no Mg2+/Ca2+, 2% Empigen) containing protease inhibitors (Roche, 11873580001) and RNasin (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... Dehydrated slides were then placed on coverslips containing 70 µL hybridization mix (10 mM Tris-HCl, 2% blocking reagent (Roche, #11096176001), 70% formamide and 0.1 µM Cy3 labeled (CCCTAA)3 PNA probe (PNA Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 2 mM CaCl2) containing 0.3 M of sucrose and MNase (30 units of MNase for every 2 million sperm, Roche Nuclease S7) was added to each tube ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (solely used for CDKN1A ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and chromatin quality analysed using EtBr agarose gel electrophoresis (1.3 % Biozym ME Agarose ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and analysed using ethidium bromide (EtBr ...
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bioRxiv - Plant Biology 2022Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... the 5-Bromo-2’-deoxy-uridine Labelling and Detection Kit II (Roche, UK) was used per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μg of total RNA and Transcriptor High Fidelity cDNA Synthesis kit (Roche). Transcript abundance was assayed by semi-quantitative PCR for two gene regions – up-stream and down-stream from the CRISPR-generated insertion (see Supplemental Fig ...
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bioRxiv - Plant Biology 2020Quote: ... with KAPA SYBR FAST qPCR Master Mix (2×) Kit (Kapa Biosystems, Wilmington, MA). Each primer pair was assessed for efficiency of amplification ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... The numbers of DNA-containing viral particles was measured using the SYBR Green I Master Kit (Roche) and a LightCycler 480 Instrument II (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse-transcribed cDNA libraries containing the TCR sequences were quantified using KAPA Library Quantification Kit (KAPA Biosystems), and loaded at 4 nM into the MiSeq sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Tail genomic DNA was extracted using a KAPA mouse genotyping Kit (KAPA Biosystems KK7352), and PCR was performed using primers as described in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted from tail tissue with a KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... DNA was extracted from tail tissue using the KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping was performed via tail clips using the KAPA Mouse Genotyping Kit (Roche #07961766001). See Table S2 for primer sequences ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...