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Citations for Roche :
351 - 400 of 837 citations for Mouse CSRNP1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of each plasmid was combined with Fugene transfection reagent (Roche Applied Science, Mannheim, Germany) at a ratio of 5 µl of transfection reagent to 1 µg DNA in 500 µl of MEM and incubated at room temperature for 30 minutes prior to addition to a 10 cm dish of HEK293 cells at 50% confluence ...
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bioRxiv - Genetics 2020Quote: ... BAC DNA from clones was isolated using a High Pure Plasmid Isolation Kit (Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Separate PLAT-E cultures were transfected with 9µg of each pMX plasmid using FuGENE 6 (Roche) for retroviral production ...
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bioRxiv - Genomics 2021Quote: ... The p415-CYC1-HIS3 plasmid was linearized by inverse PCR using KAPA HiFi polymerase (Kapa Biosystems) with primers F-p415-His and R-p415-HIS (oligonucleotide sequences in Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... and were transfected with indicated plasmids by the X-tremeGENE HP DNA Transfection Reagent (Roche, USA) when the cell confluence was up to 90% ...
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bioRxiv - Molecular Biology 2022Quote: ... structure in the 5’UTR and 70 ng of firefly luciferase (FLuc)-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... or a HP50 hairpin structure in the 5’UTR and 2 ng of FLuc-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting plasmids were used for RNA probe synthesis with digoxigenin (DIG) labeling mix (Roche, #11277073910) or fluorescein (FITC ...
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bioRxiv - Microbiology 2023Quote: ... UL16 and UL21 expression plasmids using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2.5 ug plasmid DNA per well using DOTAP Liposomal Transfection Reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Physiology 2024Quote: The BSEP promoter plasmid based on pGL3-basic (BSEPprom-Luc) was a kind gift from Roche. The human SHP promoter (bases -572 to +10 ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were transfected into HeLa cells using X-tremeGENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Cell Biology 2020Quote: ... against mouse NaBC1 transporter in X-tremeGENE siRNA Transfection Reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated using a KAPA Mouse Genotyping Kit (Roche). PCR amplification of the N-terminal region of TRIM1 was performed using Q5 High-Fidelity 2X Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged proteins were detected with mouse (Covance) or rat (Roche) monoclonal anti-V5 antibodies at 1 μg/ml or 12.5 ng/ml respectively ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP clones 7.1 and 13.1 (Roche 11814460001, 1:500), rabbit anti-Cpn60 (From ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were probed with mouse anti-GFP (Roche, cat. no. 11814460001) (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche) (1:1000) ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-Myc (AB_390912, 1:100 for IF) was from Roche. Rabbit anti-V5 (#13202 ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-GFP mouse IgG (clones 7.1 and 13.1; Roche Applied Science) was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000 ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... DNA was extracted by the KAPA Mouse Genotyping Kit (KAPA Biosystems) and genotyped by Sanger sequencing.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were mouse anti-HA 12CA5 (Roche; 1:1000), mouse anti-HA 11 (Biolegend, ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies mouse mAb α-GFP (1:1,000) (Roche Diagnostics #11814460001) or mAb α-PfGAPDH 84 (1:20,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibodies used were mouse monoclonal GFP (1:500, Roche) and rabbit polyclonal BRCA1 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used were mouse monoclonal anti-GFP (Roche, 11814460001), rabbit polyclonal anti-GFP (Acris ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-HA clone 12CA5 (WB 1:2000; 11583816001) from Roche; mouse IgG isotype control (14-4732-82 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP (Roche #1814460001; WB 1:1000; IF 1:200), rabbit anti-FLAG (Cell Signaling Technology 2368 ...
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bioRxiv - Developmental Biology 2019Quote: ... and mouse monoclonal anti-BrdU antibody (1:450, Roche, Cat# 11170376001). Secondary antibody incubation was carried out over night at 4°C using Alexa Fluor 488-conjugated ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Antibodies were used as follows: (primaries) mouse anti-HA (Roche 12CA5) 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... or biotinylated horse anti-mouse (1:500, Roche Applied Science, Switzerland) with subsequent incubation with streptavidin-conjugated Alexa 568 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were: mouse anti-GFP (Roche, 11814460001, 0.4 µg/mL), rabbit anti-Cdc2 (Santa Cruz ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were detected with a mouse anti-FLAG (Roche) antibody diluted 1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Antibodies and titers used: 1:5,000 mouse anti-GFP (Roche 1814460001); 1:10,000 goat anti-mouse-HRP (Sigma A0168) ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal mouse antibodies were used to detect GFP-tagged proteins (Roche) (dilution 1:2000 ...
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bioRxiv - Plant Biology 2024Quote: ... or mouse anti-Myc:HRP antibodies (9E10 clone, Roche [dilution 1:3000]), proteins were visualized using the Bio-Rad Clarity™ Western ECL kit and the ChemiDoc Imaging System (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: mouse anti-GFP (#11814460001, Roche) rabbit anti-TACC (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... we combined 800 ng of plasmid library with 25 µL 2x Kapa library amplification mix (Roche, #KK2702), 1 µL forward primer (10 µM ...
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bioRxiv - Systems Biology 2020Quote: ... The cloning of the gRNAs sequences was performed via full plasmid PCR amplification with the KAPA (Roche) polymerase using 60-mer oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa WT cells were transfected with the guide containing plasmid using the transfection reagent XtremeGENE HP (Roche) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... the plasmid was used for in vitro RNA transcription in the presence of DIG labelling mix (Roche) and RNA Polymerase (see Table S4 ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmid for the probes were linearized and transcribed in vitro using DIG RNA labeling mix (Roche; supplementary material Table S1 for plasmid templates for RNA synthesis).
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bioRxiv - Developmental Biology 2022Quote: HEK293T at 60-80% confluence were transfected with plasmids amplified from an overexpressing lentiviral library together with 0.975µg CMVΔR8.74 and 0.525µg VGV.G packaging plasmids using X-tremeGENE™ HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Transient transfection was achieved by transfecting cells with plasmid DNAs using XtremeGene HP DNA Transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... TCR or CAR plasmid pools were used as templates for PCR amplification (KAPA HiFi HotStart ReadyMix, Roche) to generate double-stranded DNA templates including truncated Cas9 target sequences (Nguyen et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Linearized plasmids were then used as templates for in vitro transcription using either SP6 or T7 (Roche) using DIG labelled nucleotides (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche). For silencing experiments ...