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Citations for Roche :
401 - 450 of 5122 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and KAPA Library Quantification Kits (Roche) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... BrdU (colorimetric) kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol with minor modification ...
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bioRxiv - Cell Biology 2023Quote: ... The KAPA mRNA HyperPrep kit (Roche) was used for reverse transcription ...
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bioRxiv - Cancer Biology 2023Quote: ... First Strand cDNA Synthesis Kit (Roche) and Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and KAPA Library quantification kit (Roche). Clustering and sequencing were performed on a NovaSeq6000 (Illumina, ...
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bioRxiv - Immunology 2023Quote: ... quantitated (KAPPA Library Quantitation Kit, Roche) and pooled into libraries ...
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bioRxiv - Neuroscience 2023Quote: ... Cytotoxicity Detection Kit (LDH; Roche, #11644793001), Amplex Red Glutamic Acid/Glutamate Oxidase Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... and SeqCapAdapter Kit A (Roche, USA). 14 PCR cycles were used to amplify the libraries ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Using Kapa HyperPrep kits (Kapa Biosystems) and Illumina TruSeq iTru5 and iTru7 adapters 82 ...
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bioRxiv - Developmental Biology 2023Quote: ... The KAPA Library Quantification Kit (Roche) was used for quantification of ATAC-seq and RNA-seq libraries.
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bioRxiv - Cancer Biology 2024Quote: ... The following commercial kits from Roche-Ventana Medical System were used ...
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bioRxiv - Cancer Biology 2022Quote: ... The minced tissue was resuspended in DMEM supplemented with Liberase Thermolysin Medium enzyme mix (0.2 mg/ml, Roche) and DNAse I (0.2mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... The following day the predigested hearts were transferred to digestion medium (20 mM BDM, 1.5 mg/ml Roche Collagenase/Dispase enzyme mix in L15 medium ...
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bioRxiv - Microbiology 2019Quote: ... shaved skin was incubated in I10F medium in the presence of 1 U/ml of dispase (Roche Diagnostics) for 1 h at 37° C or 0.5 U/ml overnight at 4° C ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells were maintained in Myelocult medium supplemented with penicillin/streptomycin and 100 U/ml IL-2 (Roche). CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... at 95°C for 60 minutes or with a medium concentration of protease (Protease 1; Roche Tissue Diagnostics) for 4 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Immunology 2024Quote: ... RNA was harvested by removal of culture medium and addition of 200 µl of 1 M DTT (Roche) supplement Kingfisher RNA lysis buffer (Thermofisher) ...
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bioRxiv - Immunology 2024Quote: ... Dissected meninges were incubated in digestion medium (RPMI/10% FBS/80μg/mL DNase I/40μg/mL Liberase TM (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... mouse ears were cut into small pieces and transferred into Ca2+- and Mg2+-free Hank’s medium (Life Technology) supplemented with Liberase TM (0.15 mg/mL, Roche) and DNase I (0.12 mg/mL ...
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bioRxiv - Physiology 2019Quote: Serum concentrations of sex hormone-binding globulin (SHBG) and luteinizing hormone (LH) were analysed on the Roche Modular System (Roche Diagnostics, Lewes, UK) by two-site sandwich immunoassay using electrochemiluminesence technology.
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... triton X-100 in HBSS for 10 min and incubated with blocking solution containing 2% (w/v) bovine serum albumin fraction V (Roche, Woerden, The Netherlands) and 0.1% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... and the cell pellet was re-suspended in 2 ml of triturating solution (containing 10 mg/ml bovine serum albumin [A7906, Sigma], 0.5 mg/ml trypsin inhibitor [10109886001, Roche], 0.02 mg/ml deoxyribonuclease).
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bioRxiv - Cancer Biology 2021Quote: ... 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cancer Biology 2021Quote: ... The exome captured by the SeqCap wash kit and SeqCap pure capture bead kit (both from Roche). The Illumina Novaseq platform was used to sequence 150 bases (paired-end sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Plant Biology 2022Quote: ... The EMSA was carried out utilizing the manufacturer’s instructions (DIG-gel shift kit 2nd generation kit, Roche).
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Genetics 2022Quote: ... Embryos were then washed 3x 5 minutes in TBS-T (1% Tween-20 in Tris-buffered saline) and blocked for 1 hour in block solution (10% heat-inactivated sheep serum and 0.1% Roche blocking reagent in TBS-T). Roche blocking reagent was dissolved in maleic acid buffer according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: ... and subsequently incubated in antibody solution (5% Sheep serum, 1% Tween20 and 1:2500 dilution of Alkaline Phosphatase-linked α-Digoxigenin antibody, Roche, 11093274910 in TBST) for 2 h at RT ...
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bioRxiv - Physiology 2019Quote: ... Tissue was placed in ice-cold hypotonic lysis medium (HLM, 10 mM Tris, pH 7.4, 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche), minced and homogenized by 20 strokes in a glass homogenizer ...
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bioRxiv - Immunology 2021Quote: Mouse tracheal epithelial cells were isolated from tracheas digested overnight at 4 °C in Ham’s F12 medium plus pronase (1 mg/ml; Roche). Cells were cultured for 3 h on Primaria plates (Falcon ...
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bioRxiv - Cell Biology 2021Quote: To isolate mammary epithelial cells the third and fourth pairs of mammary glands were removed from 4 to 6-week-old s-SHIP–GFP transgenic (Tg11.5kb–GFP) mice, minced with scissors and digested in digestion medium (DMEM/F12, 2 mg ml−1collagenase I (Roche), 5 mg ml−1 insulin (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... 1 mL of lung dissociation medium (100 µg/mL of DNAse I, Roche; 1.25 mg/mL of Pronase, Roche; 9.2 µg/mL of Elastase ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissues were manually minced and digested in 25 mL of Dulbecco’s Modified Eagle Medium (DMEM) containing 2 mg/mL of collagenase A (Roche) and DNase I (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Small intestines were minced into small pieces and digested in RPMI medium containing 0.1 mg/ml DNase I (Roche) and 100 mg/ml Collagenase VIII (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... the tissues were finely chopped and transferred into 5ml polystyrene tubes () containing digestion buffer (5ml/sample) made with RPMI medium + Liberase TL (100μg/ml; Roche) + DNaseI (100μg/ml ...
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bioRxiv - Immunology 2022Quote: ... and the lamina propria was minced and digested in RPMI medium containing 2.5 mg/ml Liberase TL and 20 U/ml DNase I (both from Roche) at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: T cells were thawed into T-cell medium supplemented with 3,000 IU IL-2 (Proleukin) and DNAse (Pulmozyme, Roche) three to four days before coincubation with target cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The medium from each well was removed and replaced with fresh medium containing 10 µl of 12 mM MTT as per the manufacturer’s instruction (Roche). After 4 h of incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected using 90ng of each corresponding vector diluted in 10ul of opti-medium and 0.3ul of X-treme GENE HP DNA Transfection Reagent (Roche). 10ng of Renilla vector (Promega ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Genomics 2019Quote: ... using the DNA 1000 kit and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of Hiseq2500 in single read 100nt mode using the clustering and SBS v3 kit following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were subjected to total RNA extraction using a commercially available kit (High Pure kit, Roche, Indianapolis, IN). Total RNA was then used to make complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...