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Citations for Roche :
501 - 550 of 688 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked using 10% skimmed milk in PBS containing 0.1% Tween 20 (PBST) and then incubated with rat anti-HA high affinity (1:1,000; Roche) and rabbit anti-T ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10, Roche) or chicken anti-c-Myc primary antibody (A2128 ...
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bioRxiv - Physiology 2021Quote: ... Quantitative PCR for rat Cyp7a and reference gene GAPDH was performed using FastStart Essential DNA Green Master (Roche) in an Applied Biosystems theromocycler with 45 cycles of 95°C for 20 seconds ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Neonatal (P1) and adult rat back skin was cut into small pieces then digested with Liberase TL (Roche) for 1 hour at 37°C with constant shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The following primary antibodies were used for Western blotting (WB) and immunofluorescence (IF): rat anti-HA (Roche, 11867423001), mouse anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 4 % BSA in PBS) for 1 h at room temperature before being incubated with rat anti-HA (Roche) diluted either 1:250 (tsA-201cells ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were incubated for three hours in anti-HA High Affinity antibody from rat IgG (clone 3F10; Roche), used at 1:1200 dilution in TBS Tween 20 (0.1% ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 2-2.5 hours at room temperature with a rat anti-HA antibody (Roche, #11867423001) at a 1:1000 dilution ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Molecular Biology 2019Quote: ... 5–10 × 106 HEK-293 or MEF cells were washed with cold sucrose-containing solution I (0.5 M sucrose, 3 mM MgCl2 with Cocktail protease inhibitor, Roche), harvested by scraping into 1 ml solution I and sonicated in a BioRuptor for 10 cycles (15 sec ON/15 sec OFF) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1μL DTT (0.1 M) and 0,5 μL of protector rnase inhibitor (40 U/μL, Hoffmann-La Roche, Basel, Switzerland) were added and the mix incubated at 37° C per 2 min ...
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bioRxiv - Neuroscience 2021Quote: Frozen mouse hippocampi were homogenized in 0.2 ml of pre-cooled homogenization buffer (0.3 M sucrose, 10 mM MOPSNaOH and 1mM EDTA) supplemented with complete protease inhibitor cocktail (Roche). 50 µl of lysis buffer (8% SDS and 0.2 M DTT in 0.2 M Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were resuspended and bead beaten in 250 μL urea buffer (6 M urea, 1% SDS, 50 mM Tris-HCl pH 6.8, 1 mM EDTA, 2x Roche protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted with 50 mM ABC down to 0.5 M urea and then digested overnight using sequencing-grade endoproteinase GluC (Roche) at 25 °C ...
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bioRxiv - Microbiology 2019Quote: ... cells were resuspended in 500 μL of urea lysis buffer (8 M urea in 50 mM Tris pH 8.2, 75 mM NaCl, Roche Complete EDTA-free Protease Inhibitor Cocktail tablet ...
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bioRxiv - Microbiology 2020Quote: ... Other clinical urine markers were measured by using Combur10 Test®M semiquantitative test strips (Roche Diagnostics GmbH, Germany). Each test strip consists of colorimetric reaction spots for 10 markers ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Biophysics 2022Quote: ... 0.3 M NaCl) containing either 200 µM PMSF or a tablet of complete EDTA-free protease-inhibitor cocktail (Roche) and 10 ng ml−1 DNAse I ...
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bioRxiv - Cell Biology 2021Quote: Cell pellets or mouse tissues were resuspended in ice-cold 8 M urea buffer (8 M urea, 50 mM Tris pH 7.4, 50 mM NaCl) supplemented with protease and phosphatase inhibitors (Roche). The resuspended samples were sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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bioRxiv - Molecular Biology 2020Quote: ... The digested RNA samples were then analyzed by electrophoresis (5.5% PAGE with 8 M urea) and northern blotting using the DIG Northern Starter Kit (Roche). Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in Buffer A (2 M NaCl, 20 mM HEPES pH 7.5, 20 mM Imidazole, 5 mM βME, protease inhibitor (Roche)) by sonication ...
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bioRxiv - Biochemistry 2020Quote: ... The cell pellets were mixed with 10-fold volume of sucrose buffer (0.25 M sucrose; protease and phosphatase inhibitor cocktails (Roche)) and homogenized until a completely homogenous solution was obtained ...
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bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
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bioRxiv - Immunology 2022Quote: ... were lysed in 400 μl lysis buffer in urea buffer (8 M urea, 50 mM Tris pH 8.0, 1 mM TCEP) with protease (cOmplete mini EDTA free, Roche) and with phosphatase inhibitors (PhosStop ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old tmk1-1 roots expressing TMK1::TMK1-FLAG were grounded to powder in liquid nitrogen and homogenized in ice-cold sucrose buffer (20 mM Tris–HCl pH 8, 0.33 M sucrose, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... the water was aspirated and Triton X-100 buffer (Triton X-100, 0.5 M EDTA, PBS, ddH2O) with phosphatase (Roche) and protease (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were resuspended in microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche)) and repelleted by centrifugation at 100□000 g for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... B-R18 labelled A1 EVs were resuspended in PBS buffer (0.2 M NaCl) containing a protease inhibitory cocktail (Complete Protease Inhibitory Tablets, Roche) and were stored at −20°C.
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 1 ml SEH buffer (20 mM HEPES pH 7.4, 1 mM EDTA, 0.6 M Sorbitol, 1x cOmplete™ Tablets mini EDTA-free protease inhibitor [Roche]). Resuspended yeast cells were transferred into screw cap tubes containing 500 µl glass beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2024Quote: ... Cell pellets were resuspended in lysis buffer (0.5 M NaCl, 20 mM Na3PO4 pH 7.4 and cOmplete™ Protease Inhibitor Cocktail (Roche) and then lysed using sonication ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...