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Citations for Roche :
1 - 50 of 816 citations for M CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Cell lysates from flow-sorted bone marrow osteoclast progenitors grown in presence of M-CSF and RANKL over time were harvested in 1% NP40 lysis buffer containing 1X cOmplete Protease Inhibitor cocktail (Roche). Samples were separated by SDS-PAGE and transferred to nitrocellulose membrane ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Neuroscience 2021Quote: ... CSF p-tau181 was quantified using Elecsys immunoassays (Roche, Basel, Switzerland), while CSF p-tau217 was not available.80 For concordance analyses (in BioFINDER-2 only) ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T and CHO-K1 cells were transfected with the FuGENE6 transfection reagent (Roche Applied Bioscience) and selected by the addition of puromycin (1 μg/ml and 10 μg/ml respectively ...
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bioRxiv - Genetics 2022Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) and cOmplete protease inhibitors (Roche) was added to prevent protein degradation ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) with cOmplete™ protease inhibitors (Roche). Tissue lysates were centrifuged at 10,000 × g for 5 min at 4 °C and supernatants were collected for further testing ...
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bioRxiv - Immunology 2021Quote: Cells were collected in RIPA buffer (0.5 M EDTA, 1 M Tris-Cl pH7.5, 1 M NaCl, 200 mM, Roche protease inhibitor) at 0h ...
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bioRxiv - Systems Biology 2019Quote: CHO cells and NIH 3T3 cells were grown in standard growth medium and transfected with Fugene (Roche) or Lipofectamine 2000 (Life Sciences).
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bioRxiv - Immunology 2020Quote: ... Cytokines were added at the following concentrations: GM-CSF (5 ng/mL, Roche), TNF (10 ng/mL ...
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bioRxiv - Genetics 2021Quote: ... extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D) coated with anti-GFP antibodies (Roche ref:11814460001). Proteins extracts were resolved on 4-15% polyacrylamide gels ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche). Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL ...
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bioRxiv - Microbiology 2020Quote: ... 70-80% confluent CHO-K1 cells were transfected in 35 mm dishes using X-TREMEGENE 9 Transfection Reagent (Roche, 6365787001). Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco ...
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bioRxiv - Immunology 2019Quote: Viral RNA was extracted from serum and CSF using the High Pure Viral RNA Kit (Roche). Semen ...
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bioRxiv - Neuroscience 2023Quote: ... and tTau in CSF was measured using the fully automated Elecsys immunoassays (Roche Diagnostics, Basel, Switzerland) by the ADNI biomarker core (University of Pennsylvania ...
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bioRxiv - Neuroscience 2020Quote: ... Forebrains were finely chopped and then homogenized in 0.32 M sucrose solution (0.32 M sucrose, 10 mM Hepes pH 7.4, with cOmplete protease (Roche) and phosphatase inhibitors ...
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bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatant is the soluble fraction and the insoluble fraction is generated by resuspending the pellet in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Roche), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2021Quote: ... 8 M urea and protease inhibitor (Roche, 05892791001) with sonication on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05 M TRIS-HCl (Roche Cat. No. 10812846001), 0.8 mM EDTA (Sigma Aldrich Cat No ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.25 M KCl) supplemented with protease inhibitors (Roche) and sonicated 3x5 seconds using a Bioruptor (Diagenode) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Levels of CSF Aβ42 and phosphorylated tau (p-tau) were measured with the fully automated Elecsys immunoassay (Roche Diagnostics) by the ADNI biomarker core (University of Pennsylvania) ...
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bioRxiv - Microbiology 2019Quote: ... RNA was manually extracted from fluid samples (CSF or blood serum) using the High Pure Viral RNA Kit (Roche). RNA was then subjected to reverse transcription and quantitative PCR using primers and a fluorescently conjugated probe on an Applied Biosystems 7900 instrument.
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Biophysics 2020Quote: ... EDTA-free lysis-M buffer with protease inhibitor (Roche). Protein concentrations were determined using PierceTM BCA kit (Thermo ScientififTM) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.15 M NaCl and Complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Immunology 2021Quote: ... hydrocortisone hemisuccinate (7×10−5 M, Roche-Boehringer-Manheim) and FCS (10% selected ...
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bioRxiv - Neuroscience 2019Quote: ... 0.15 M NaCl and complete protease inhibitor cocktail (Roche) by ultrasonication ...
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bioRxiv - Cell Biology 2023Quote: ... 0.3 M sucrose containing Complete Protease Inhibitor Cocktail (Roche). Suspended cells were homogenized using a syringe with a 27-gauge needle ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue was chopped and incubated for 30 min in artificial CSF (pH 7.4) supplemented with 1x complete mini EDTA-free protease inhibitor cocktail tablet (Roche, 11836170001). The was centrifuged at 2000 RCF for 10 mins to remove large ...
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bioRxiv - Neuroscience 2021Quote: ... the CSF Aβ42/40 ratio was determined using the MSD platform (Meso Scale Discovery) and Elecsys immunoassays (Roche Diagnostics, Basel), respectively.
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bioRxiv - Neuroscience 2023Quote: ... containing 10mL 1X artificial CSF (pH 7.4) supplemented with 1x cOmplete mini EDTA-free protease inhibitor cocktail tablet (Roche, 11836170001) per 10mL ...
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bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and sonicated for 10 s at 20% amplitude using a Sonics Vibra Cell sonicator fitted with a microprobe ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
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bioRxiv - Biophysics 2022Quote: ... 0.02% NaN3 and 1x cOmpleteT M Protease Inhibitor Cocktail (Roche). 10% D2O was added for lock.
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bioRxiv - Microbiology 2021Quote: ... 2 M CaCl2 and 2 UU/ml DNAse I (Roche) for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and lysates were sonicated for 10 s at 20% amplitude using a Vibra-Cell sonicator (Sonics ...
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bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 M NaCl with Complete protease inhibitor tablet (Roche, Indianapolis, IN) and centrifuged for 30 min at 13,000 rpm 4°C to pellet cell debris ...