Labshake search
Citations for Roche :
151 - 200 of 742 citations for Lassa Fever Virus GP2 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
-
bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
-
bioRxiv - Microbiology 2021Quote: We then targeted the N-(nucleoprotein) gene of SARS-CoV-2 virus with Roche Lightcycler 480 RNA Master Hydrolysis Probes (Roche Basel, Switzerland) with primers developed in house ...
-
bioRxiv - Microbiology 2020Quote: We obtained OP and NP samples from one hospitalised COVID-19 patient who had tested virus-positive three weeks earlier (cobas® SARS-CoV-2 test, Roche diagnostics), from three individuals who had previously tested SARS-CoV-2 positive ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
-
bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
-
bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... spleens and were minced and digested in 5 mL of Iscove’s modified Dulbecco’s media (IMDM) + 10% fetal calf serum (FCS) (cIMDM) with 250 μg/mL collagenase B (Roche) and 30 U/mL DNaseI (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
-
bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
-
bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
-
bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
-
bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Cell suspensions were first pre-incubated with 2.4G2 antibody (kindly provided by Louis Boon) for blocking Fc receptors and next stained for surface markers in PBS supplemented with 0.5% BSA (Roche) and 2 mM EDTA (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
-
bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
-
bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
-
bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
-
bioRxiv - Immunology 2022Quote: ... Tissue pieces then were transferred to a 50-mL conical tube containing 5 ml of digestion medium #2 (RPMI without phenol red containing 5% of FCS, 25 µg/ml of Liberase (Roche), and 200 µg/ml of DNase I (Sigma)) ...
-
bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
-
bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
-
bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
-
Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
-
bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
-
bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
-
bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
-
bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
-
bioRxiv - Immunology 2021Quote: ... were thawed by washing twice in pre-warmed RPMI1640 medium supplemented with 20% FCS and 10 ng/mL DNAse I (Roche, 11284932001). Thawed CAR T cells were rested in cytokine-free complete medium (RPMI1640 medium containing 10% FCS and 1% penicillin-streptomycin ...
-
bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
-
bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein G agarose beads (Roche #11719416001) pre-blocked with salmon sperm (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... together with protein A agarose (Roche) overnight at 4 ℃ ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein G agarose beads (11243233001, Roche) were added to the lysates and incubated for additional 4 hours at 4°C with rotary agitation ...
-
bioRxiv - Neuroscience 2021Quote: ... homogeneous protein G-agarose suspension (Roche) was added to the suspension ...
-
bioRxiv - Microbiology 2019Quote: ... Protein G-conjugated agarose beads (Roche) or PrecipHen for chicken antibodies (Aves Labs ...
-
bioRxiv - Cell Biology 2019Quote: ... protein inhibitor cocktail (Roche, Mannheim, Germany) and then sonicated ...