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Citations for Roche :
201 - 250 of 732 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... HindIII treatment was performed at 37°C for 1 h in 1x reaction buffer B with 10U of HindIII enzyme per reaction (HindIII, 10656321001, Roche, Switzerland). Mung bean nuclease treatment was performed at 30°C for 30 min in 1x mung bean nuclease reaction buffer with 1U per reaction (Mung Bean Nuclease ...
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bioRxiv - Biophysics 2022Quote: ... embryos were washed (4 X 10 min) in PBTx and blocked in PBT-B (1× PBS, 20% (v/v) western blocking reagent (Roche, 11921673001), 2 mM ribonucleoside vanadyl complex (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... using the Elecsys® Intact hCG+b electrochemiluminescence immunoassay (ECLIA) and the fully automated modular analytics E170 testing system from Roche Diagnostics (Mannheim ...
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bioRxiv - Biochemistry 2023Quote: ... Selection for dominant markers was performed on YPD-based medium supplemented with 200 μg/mL G418 (Beyotime, ST081), 250 μg/mL clonNAT (KLBIOS, PD20181786) or 300 μg/mL hygromycin B (Roche, 10843555001).
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Physiology 2020Quote: ... Effluent levels of LDH were measured using a cytotoxicity detection kit (Roche). Normalized absorbance values (absorbance at 492 nm minus absorbance at 690 nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... LDH release was evaluated in supernatants using a cytotoxicity detection kit (Roche).
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bioRxiv - Cell Biology 2022Quote: ... Cell death was measured by a cytotoxicity detection kit (LDH) from Roche to assess lactate dehydrogenate activity in the culture supernatants of ACE2plus cells.
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bioRxiv - Molecular Biology 2021Quote: ... Putative hygromycin and neomycin-resistant candidates transformants were screened on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Screening of hygromycin- and neomycin-resistant transformants was performed on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1, LS005477] and collagenase type B [Roche, 0.35 U·ml-1, 11088807001]). The left ventricle was collected after 10 minutes’ digestion and cut into small pieces ...
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bioRxiv - Neuroscience 2019Quote: ... Tongues were removed and injected beneath the lingual epithelium with 0.2 mL enzyme solution (3 mg Dispase II, 0.7 mg collagenase B (Roche diagnostics, Indianapolis, IN, USA) and 1 mg Trypsin Inhibitor in 1mL Tyrode’s) ...
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bioRxiv - Cell Biology 2020Quote: ... Telomeric repets were calculated as described (Gil and Coetzer, 2004a, b; Vasilishina et al., 2019) using a LightCycler 2,0 (Roche Diagnostics, Basilea, SWI). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... Viability of the tissue was assessed by an LDH Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were centrifuged and cell killing assessed using the LDH Assay (Roche #11644793001) according to the manufacturers protocol using the SPECTROstar Nano (BMG Labtech).
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bioRxiv - Microbiology 2022Quote: ... LDH release levels were analyzed using a Cytotoxicity Detection Kit (Roche, Basel, Switzerland). Purified Ply(1μM)was co-cultured with various concentrations of alnustone at 37°C for 30min ...
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bioRxiv - Microbiology 2022Quote: ... LDH in the cell supernatants was quantified using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... LDH released in the supernatant was detected using a cytotoxicity detection kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cytotoxicity was evaluated by measuring LDH release according to the manufacturer’s protocol (Roche). S180 cell line was used as the unrelated control ...
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bioRxiv - Cell Biology 2022Quote: ... and His-tag (11965085001, Roche) antibodies were used to detect the proteins.
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bioRxiv - Biochemistry 2019Quote: ... anti-His-tag (11922416001; Roche), anti-vinculin (V4505 ...
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bioRxiv - Immunology 2021Quote: ... HCMV glycoprotein B gene (gB) in the DNA sample was amplified in Lightcycler 480 real time PCR platform (Roche Molecular System, Pleasanton, CA) using TaqMan probe (LightCycler TaqMan Master ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified by using a High Pure PCR product purification kit (B A High Pure PCR Product Purification Kit (Roche Diagnostics, Mannheim, Germany), and purified products were sequenced directly using an ABI BigDye Terminator Cycle Sequencing Kit ver ...
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bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Lactate concentration was determined at 37 ºC with 2.35 mg/ml NAD+ (Roche) in 0.15 M hydrazine 10.9 mM EDTA pH 9 buffer and 80 U/ml lactate dehydrogenase (Roche ...
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bioRxiv - Physiology 2020Quote: ... supernatant was collected and LDH levels were measured using a cytotoxicity detection kit (Roche). For all cell culture experiments ...
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bioRxiv - Biochemistry 2019Quote: ... including all recommended controls (LDH; Cytotoxicity Detection Kit Plus, Roche Applied Sciences, Penzberg, Germany). Cell-free culture medium was measured as background ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were assayed for LDH activity using the Cytotoxicity Detection Kit (Roche, Indianapolis, IN) (8,20) ...
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bioRxiv - Immunology 2023Quote: Cell viability was assessed using the Cytotoxicity Detection Kit Plus (LDH) (Roche, Cat#: 04744926001) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 0.1% Tween-20, 0,5% Triton X−100, 1 mM DTT, 1× protease inhibitors cocktail, complete EDTA-free, Roche and antifoam B, Sigma, 1:5000). The obtained extract was clarified by filtration through 1.6 μm GD/X Glass Microfiber syringe filters (25 mm ...
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bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Plant Biology 2022Quote: ... The glucose released was measured using the hexokinase/glucose-6-phosphate dehydrogenase method (Roche). Starch content (in glucose equivalents ...
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bioRxiv - Plant Biology 2023Quote: ... and released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche).
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bioRxiv - Biochemistry 2021Quote: ... cOmplete His-Tag Purification Resin (Roche) was added to the supernatant and samples were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... Lactate levels were measured using Cobas C311 Analyzer with LACT2 kits (Roche Diagnostics GmbH). Human Precinorm y Precipath were used as controls.
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Cell Biology 2020Quote: Cell stress was assessed by LDH release using the Roche Cytotoxicity Detection Kit (Roche 11644793001) in cells treated with OxPC (0-160 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Data was analyzed in triplicate using LDH cytotoxicity detection kit (Roche Applied Science, Mannheim, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability of the tissue was assessed by a LDH Cytotoxicity Detection Kit (Roche, Mannheim, Germany) according to manufacturer’s instruction.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The activity of LDH in the medium was determined using a commercially available kit (Roche) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of LDH released from cells were determined with a Cytotoxicity Detection Kit (Roche), as described previously 46.
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bioRxiv - Biophysics 2023Quote: ... Cell Proliferation Reagent WST-1 and Cytotoxicity Detection KitPLUS (LDH) were obtained from Roche (Germany). 5× HOT FIREPol® EvaGreen® qPCR Supermix was acquired from Solis BioDyne (Estonia) ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Glucose was measured in the supernatant using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche), for calculation of starch content in glucose equivalents ...
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bioRxiv - Plant Biology 2023Quote: ... and the released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche). For mature grains ...